CPS2_ORYSI
ID CPS2_ORYSI Reviewed; 800 AA.
AC Q5MQ85;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Ent-copalyl diphosphate synthase 2;
DE Short=Ent-CDP synthase 2;
DE Short=OsCPS2;
DE Short=OsCPS2ent;
DE EC=5.5.1.13;
DE AltName: Full=Ent-kaurene synthase A;
DE AltName: Full=OsCyc2;
GN Name=CPS2; Synonyms=CYC2; ORFNames=OsI_007598;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INDUCTION.
RC STRAIN=cv. IR24;
RX PubMed=15542489; DOI=10.1104/pp.104.050567;
RA Prisic S., Xu M., Wilderman P.R., Peters R.J.;
RT "Rice contains two disparate ent-copalyl diphosphate synthases with
RT distinct metabolic functions.";
RL Plant Physiol. 136:4228-4236(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: Catalyzes the conversion of geranylgeranyl diphosphate to the
CC phytoalexin precursor ent-copalyl diphosphate.
CC {ECO:0000269|PubMed:15542489}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E,10E)-geranylgeranyl diphosphate = ent-copalyl
CC diphosphate; Xref=Rhea:RHEA:14841, ChEBI:CHEBI:58553,
CC ChEBI:CHEBI:58756; EC=5.5.1.13;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305};
CC -!- INDUCTION: By UV irradiation. {ECO:0000269|PubMed:15542489}.
CC -!- DOMAIN: The Asp-Xaa-Asp-Asp (DXDD) motif is important for the catalytic
CC activity, presumably through binding to Mg(2+).
CC -!- MISCELLANEOUS: 2 different ent-CDP synthases exist in rice, one being
CC involved in gibberellin biosynthesis and the other in phytoalexins
CC biosynthesis. Phytoalexins are diterpenoid secondary metabolites
CC involved in the defense mechanism of the plant and produced in response
CC to attack (by a pathogen, elicitor or UV irradiation).
CC -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
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DR EMBL; AY602991; AAT11021.1; -; mRNA.
DR EMBL; CM000127; EAY86365.1; -; Genomic_DNA.
DR AlphaFoldDB; Q5MQ85; -.
DR SMR; Q5MQ85; -.
DR STRING; 39946.Q5MQ85; -.
DR EnsemblPlants; BGIOSGA008469-TA; BGIOSGA008469-PA; BGIOSGA008469.
DR Gramene; BGIOSGA008469-TA; BGIOSGA008469-PA; BGIOSGA008469.
DR HOGENOM; CLU_003125_3_2_1; -.
DR OMA; RDKWVIA; -.
DR BRENDA; 5.5.1.13; 4460.
DR Proteomes; UP000007015; Chromosome 2.
DR GO; GO:0009905; F:ent-copalyl diphosphate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SUPFAM; SSF48239; SSF48239; 2.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 2: Evidence at transcript level;
KW Isomerase; Magnesium; Metal-binding; Plant defense; Reference proteome.
FT CHAIN 1..800
FT /note="Ent-copalyl diphosphate synthase 2"
FT /id="PRO_0000372327"
FT REGION 52..80
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 374..377
FT /note="DXDD motif"
FT COMPBIAS 52..73
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 242
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q38802"
FT BINDING 374
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:C7BKP9"
FT BINDING 376
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:C7BKP9"
FT BINDING 461
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q38802"
SQ SEQUENCE 800 AA; 90006 MW; CB19FE6EEE1AB0BE CRC64;
MQMQVLTAAS SLPRATLLRP AAAEPWRQSF LQLQARPIQR PGIMLHCKAQ LQGQETRERR
QLDDDEHARP PQGGDDDVAA STSELPYMIE SIKSKLRAAR NSLGETTVSA YDTAWIALVN
RLDGGGERSP QFPEAIDWIA RNQLPDGSWG DAGMFIVQDR LINTLGCVVA LATWGVHEEQ
RARGLAYIQD NLWRLGEDDE EWMMVGFEIT FPVLLEKAKN LGLDINYDDP ALQDIYAKRQ
LKLAKIPREA LHARPTTLLH SLEGMENLDW ERLLQFKCPA GSLHSSPAAS AYALSETGDK
ELLEYLETAI NNFDGGAPCT YPVDNFDRLW SVDRLRRLGI SRYFTSEIEE YLEYAYRHLS
PDGMSYGGLC PVKDIDDTAM AFRLLRLHGY NVSSSVFNHF EKDGEYFCFA GQSSQSLTAM
YNSYRASQIV FPGDDDGLEQ LRAYCRAFLE ERRATGNLRD KWVIANGLPS EVEYALDFPW
KASLPRVETR VYLEQYGASE DAWIGKGLYR MTLVNNDLYL EAAKADFTNF QRLSRLEWLS
LKRWYIRNNL QAHGVTEQSV LRAYFLAAAN IFEPNRAAER LGWARTAILA EAIASHLRQY
SANGAADGMT ERLISGLASH DWDWRESNDS AARSLLYALD ELIDLHAFGN ASDSLREAWK
QWLMSWTNES QGSTGGDTAL LLVRTIEICS GRHGSAEQSL KNSEDYARLE QIASSMCSKL
ATKILAQNGG SMDNVEGIDQ EVDVEMKELI QRVYGSSSND VSSVTRQTFL DVVKSFCYVA
HCSPETIDGH ISKVLFEDVN
