ID   CPS3_ISORU              Reviewed;         746 AA.
AC   A0A1X9IRT6; A0A1W6QDH6;
DT   07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT   30-AUG-2017, sequence version 1.
DT   03-AUG-2022, entry version 20.
DE   RecName: Full=Probably inactive copalyl diphosphate synthase 3 {ECO:0000303|PubMed:28381502};
DE            Short=IrCPS3 {ECO:0000303|PubMed:28381502};
DE   AltName: Full=Probably inactive diterpene synthase 7 {ECO:0000303|PubMed:28445526};
DE            Short=IrTPS7 {ECO:0000303|PubMed:28445526};
GN   Name=CPS3 {ECO:0000303|PubMed:28381502};
GN   Synonyms=TPS7 {ECO:0000303|PubMed:28445526};
OS   Isodon rubescens (Rabdosia rubescens).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Lamiales; Lamiaceae; Nepetoideae; Ocimeae; Isodoninae;
OC   Isodon.
OX   NCBI_TaxID=587669;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX   PubMed=28381502; DOI=10.1104/pp.17.00202;
RA   Jin B., Cui G., Guo J., Tang J., Duan L., Lin H., Shen Y., Chen T.,
RA   Zhang H., Huang L.;
RT   "Functional diversification of kaurene synthase-like genes in Isodon
RT   rubescens.";
RL   Plant Physiol. 174:943-955(2017).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-530.
RX   PubMed=28445526; DOI=10.1371/journal.pone.0176507;
RA   Pelot K.A., Hagelthorn L.M., Addison J.B., Zerbe P.;
RT   "Biosynthesis of the oxygenated diterpene nezukol in the medicinal plant
RT   Isodon rubescens is catalyzed by a pair of diterpene synthases.";
RL   PLoS ONE 12:e0176507-e0176507(2017).
CC   -!- TISSUE SPECIFICITY: Mostly expressed in stems, and, at low levels, in
CC       roots and leaves, but barely in flowers. {ECO:0000269|PubMed:28381502}.
CC   -!- DOMAIN: The Asp-Xaa-Asp-Asp (DXDD) motif is important for the catalytic
CC       activity, presumably through binding to Mg(2+). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the terpene synthase family. Tpsc subfamily.
CC       {ECO:0000305}.
CC   -!- CAUTION: Probably inactive enzyme; missing the second Asp of the DXDD
CC       motif that has been reported to be essential in forming hydrogen-bonded
CC       'proton wires' during catalysis. {ECO:0000305|PubMed:28381502}.
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DR   EMBL; KU180501; APJ36373.1; -; mRNA.
DR   EMBL; KY661363; ARO38146.1; -; mRNA.
DR   AlphaFoldDB; A0A1X9IRT6; -.
DR   SMR; A0A1X9IRT6; -.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR   GO; GO:0042214; P:terpene metabolic process; IEA:UniProt.
DR   GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProt.
DR   Gene3D; 1.10.600.10; -; 1.
DR   Gene3D; 1.50.10.130; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR001906; Terpene_synth_N.
DR   InterPro; IPR036965; Terpene_synth_N_sf.
DR   InterPro; IPR005630; Terpene_synthase_metal-bd.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   Pfam; PF01397; Terpene_synth; 1.
DR   Pfam; PF03936; Terpene_synth_C; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   2: Evidence at transcript level;
FT   CHAIN           1..746
FT                   /note="Probably inactive copalyl diphosphate synthase 3"
FT                   /id="PRO_0000452392"
FT   MOTIF           331..334
FT                   /note="DXDD motif; degenerated"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        231
FT                   /note="L -> F (in Ref. 2; ARO38146)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        288
FT                   /note="A -> T (in Ref. 2; ARO38146)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        361
FT                   /note="D -> N (in Ref. 2; ARO38146)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        446
FT                   /note="E -> K (in Ref. 2; ARO38146)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        524..530
FT                   /note="LAAASIF -> FGGCKHI (in Ref. 2; ARO38146)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   746 AA;  85202 MW;  C96B95E0B5DC5C63 CRC64;
     MDDNYRIFNK NLITNAARRG TPQVCTGNGG FEPQPFDKEY VLQKNKIEEC ITKTLQSIGD
     GRISASAYDT AWIALIKDVI NRDLPLFPWS LEWIVSNQLR DGSWGDRDFF VPCDRLLSTL
     ACVVALSTWN VHPNETERGI LFVKENISKL EDGDDVHTTL GFEILFPALL ERARNLGIEG
     LPYDDPTTQK ICAERDLKLD RISKELIINE VPASLLLILE GLENLNWENI LKLQSPDGSF
     LGSPATTAFV FMETKDESCL SYLKKTVQNF GGGAPSLYPV DILTRLLAVD RLQRLGISRF
     FHSQIEDCLS YIYRFWTGNG VFSGRDSEFC DVNDTSMAFR LLRLHGYNVS PNVFTNFKKG
     DEFSVYGDDE VVDSPSTMLN LYRASEVRFA GETILEEAKE FSHNFLQQRQ GLPNQVLDNC
     LISKNLHTEI KYELETPWFA SLPRLEARFF IERYNAVDEV CIGKSLYRLP DTNEGTYLEL
     AKLDYNRCQE QHQMEWNHMQ QWYEDCNLEE FGISKNDILE AHFLAAASIF EAERSGERVA
     WVKTQILSHI LSTYYFIKQS HQDHKPQLST EKAHIAGQPG KGFKNVQRII SILFEALTQM
     KKDALEGSNG DISDDLLHEA WGGWLKKLGE GETEERQEAE LIARTINVCG GHILSKQILS
     HHEYKTLSQL TNQICHNLHN SKMIGKEMEN EMQLLVQLVL QESSNGISKA IKQTFLVVAK
     AFYYRAYFSA KQIENHVSII LFEQLV