CPSB_STREE
ID CPSB_STREE Reviewed; 243 AA.
AC Q54518; O08049; O08278; O52232;
DT 01-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 88.
DE RecName: Full=Tyrosine-protein phosphatase CpsB;
DE EC=3.1.3.48;
GN Name=cpsB; Synonyms=cap1B, cps19fB;
OS Streptococcus pneumoniae.
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=1313;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Serotype 19F;
RX PubMed=7960118; DOI=10.1128/iai.62.12.5384-5396.1994;
RA Guidolin A., Morona J.K., Morona R., Hansman D., Paton J.C.;
RT "Nucleotide sequence analysis of genes essential for capsular
RT polysaccharide biosynthesis in Streptococcus pneumoniae type 19F.";
RL Infect. Immun. 62:5384-5396(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11902728; DOI=10.1046/j.1365-2958.1997.4341801.x;
RA Munoz R., Mollerach M.E., Lopez R., Garcia E.;
RT "Molecular organization of the genes required for the synthesis of type 1
RT capsular polysaccharide of Streptococcus pneumoniae: formation of binary
RT encapsulated pneumococci and identification of cryptic dTDP-rhamnose
RT biosynthesis genes.";
RL Mol. Microbiol. 25:79-92(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=NCTC 11906 / Serotype 19F, PO-329 / Serotype 19F,
RC SP-496 / Serotype 19F, SP-GA71 / Serotype 19F, SP-VA92 / Serotype 19F, and
RC SP-VA96 / Serotype 19F;
RX PubMed=9466257; DOI=10.1046/j.1365-2958.1998.00658.x;
RA Coffey T.J., Enright M.C., Daniels M., Morona J.K., Morona R.,
RA Hryniewicz W., Paton J.C., Spratt B.G.;
RT "Recombinational exchanges at the capsular polysaccharide biosynthetic
RT locus lead to frequent serotype changes among natural isolates of
RT Streptococcus pneumoniae.";
RL Mol. Microbiol. 27:73-83(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 220-243.
RC STRAIN=Serotype 19B;
RA Morona J.K., Morona R., Paton J.C.;
RT "Analysis of capsule loci from various Streptococcus pneumoniae serotypes
RT using long-range PCR identifies two classes of cpsC.";
RL Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP CHARACTERIZATION, AND MUTAGENESIS OF ASP-199 AND HIS-201.
RC STRAIN=Rx1-19F / Serotype 19F;
RX PubMed=11751838; DOI=10.1128/jb.184.2.577-583.2002;
RA Morona J.K., Morona R., Miller D.C., Paton J.C.;
RT "Streptococcus pneumoniae capsule biosynthesis protein CpsB is a novel
RT manganese-dependent phosphotyrosine-protein phosphatase.";
RL J. Bacteriol. 184:577-583(2002).
CC -!- FUNCTION: Dephosphorylates CpsD. Involved in the regulation of capsular
CC polysaccharide biosynthesis.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] +
CC phosphate; Xref=Rhea:RHEA:10684, Rhea:RHEA-COMP:10136, Rhea:RHEA-
CC COMP:10137, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:46858,
CC ChEBI:CHEBI:82620; EC=3.1.3.48;
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC -!- PATHWAY: Capsule biogenesis; capsule polysaccharide biosynthesis.
CC -!- SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily.
CC CpsB/CapC family. {ECO:0000305}.
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DR EMBL; U09239; AAC44959.1; -; Genomic_DNA.
DR EMBL; Z83335; CAB05935.1; -; Genomic_DNA.
DR EMBL; AF030367; AAC38717.1; -; Genomic_DNA.
DR EMBL; AF030368; AAC38722.1; -; Genomic_DNA.
DR EMBL; AF030369; AAC38727.1; -; Genomic_DNA.
DR EMBL; AF030370; AAC38731.1; -; Genomic_DNA.
DR EMBL; AF030371; AAC38736.1; -; Genomic_DNA.
DR EMBL; AF030372; AAC38741.1; -; Genomic_DNA.
DR EMBL; AF106137; AAD17985.1; -; Genomic_DNA.
DR RefSeq; WP_000565348.1; NZ_WNIA01000046.1.
DR RefSeq; WP_000565349.1; NZ_FYMI01000027.1.
DR AlphaFoldDB; Q54518; -.
DR SMR; Q54518; -.
DR OMA; VHPERNS; -.
DR UniPathway; UPA00934; -.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR GO; GO:0004725; F:protein tyrosine phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0045227; P:capsule polysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR016667; Caps_polysacc_synth_CpsB/CapC.
DR InterPro; IPR032466; Metal_Hydrolase.
DR PANTHER; PTHR39181; PTHR39181; 1.
DR Pfam; PF19567; CpsB_CapC; 1.
DR PIRSF; PIRSF016557; Caps_synth_CpsB; 1.
DR SUPFAM; SSF51556; SSF51556; 1.
PE 1: Evidence at protein level;
KW Capsule biogenesis/degradation; Exopolysaccharide synthesis; Hydrolase;
KW Manganese; Protein phosphatase.
FT CHAIN 1..243
FT /note="Tyrosine-protein phosphatase CpsB"
FT /id="PRO_0000057892"
FT VARIANT 226
FT /note="A -> V (in strain: NCTC 11906)"
FT MUTAGEN 199
FT /note="D->N: Loss of activity; when associated with Q-201."
FT /evidence="ECO:0000269|PubMed:11751838"
FT MUTAGEN 201
FT /note="H->Q: Loss of activity; when associated with N-199."
FT /evidence="ECO:0000269|PubMed:11751838"
SQ SEQUENCE 243 AA; 28354 MW; 810A4C802EC43079 CRC64;
MIDIHSHIVF DVDDGPKSRE ESKALLAESY RQGVRTIVST SHRRKGMFET PEEKIAENFL
QVREIAKEVA DDLVIAYGAE IYYTLDALEK LEKKEIPTLN DSRYALIEFS MHTSYRQIHT
GLSNILMLGI TPVIAHIERY DALENNEKRV RELIDMGCYT QINSYHVSKP KFFGEKYKFM
KKRARYFLER DLVHVVASDM HNLDSRPPYM QQAYDIIAKK YGAKKAKELF VDNPRKIIMD
QLI
