CPSD_STRA1
ID CPSD_STRA1 Reviewed; 232 AA.
AC Q3K0T0; Q93TJ2; Q9ALX5; Q9S0S7;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Tyrosine-protein kinase CpsD;
DE EC=2.7.10.2;
GN Name=cpsD; Synonyms=cpsIaD; OrderedLocusNames=SAK_1259;
OS Streptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC
OS SS700).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=205921;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=OI1 / Serotype Ia;
RX PubMed=10464185; DOI=10.1128/jb.181.17.5176-5184.1999;
RA Yamamoto S., Miyake K., Koike Y., Watanabe M., Machida Y., Ohta M.,
RA Iijima S.;
RT "Molecular characterization of type-specific capsular polysaccharide
RT biosynthesis genes of Streptococcus agalactiae type Ia.";
RL J. Bacteriol. 181:5176-5184(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27591 / A909 / CDC SS700;
RX PubMed=16172379; DOI=10.1073/pnas.0506758102;
RA Tettelin H., Masignani V., Cieslewicz M.J., Donati C., Medini D.,
RA Ward N.L., Angiuoli S.V., Crabtree J., Jones A.L., Durkin A.S., DeBoy R.T.,
RA Davidsen T.M., Mora M., Scarselli M., Margarit y Ros I., Peterson J.D.,
RA Hauser C.R., Sundaram J.P., Nelson W.C., Madupu R., Brinkac L.M.,
RA Dodson R.J., Rosovitz M.J., Sullivan S.A., Daugherty S.C., Haft D.H.,
RA Selengut J., Gwinn M.L., Zhou L., Zafar N., Khouri H., Radune D.,
RA Dimitrov G., Watkins K., O'Connor K.J., Smith S., Utterback T.R., White O.,
RA Rubens C.E., Grandi G., Madoff L.C., Kasper D.L., Telford J.L.,
RA Wessels M.R., Rappuoli R., Fraser C.M.;
RT "Genome analysis of multiple pathogenic isolates of Streptococcus
RT agalactiae: implications for the microbial 'pan-genome'.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:13950-13955(2005).
RN [3]
RP FUNCTION.
RC STRAIN=515 / Serotype Ia;
RX PubMed=11027683; DOI=10.1074/jbc.m005702200;
RA Cieslewicz M.J., Kasper D.L., Wang Y., Wessels M.R.;
RT "Functional analysis in type Ia group B Streptococcus of a cluster of genes
RT involved in extracellular polysaccharide production by diverse species of
RT Streptococci.";
RL J. Biol. Chem. 276:139-146(2001).
CC -!- FUNCTION: Involved in the regulation of capsular polysaccharide
CC biosynthesis. Autophosphorylation of CpsD attenuates its activity and
CC reduces the level of encapsulation. May be part of a complex that
CC directs the coordinated polymerization and export to the cell surface
CC of the capsular polysaccharide. {ECO:0000269|PubMed:11027683}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-
CC [protein]; Xref=Rhea:RHEA:10596, Rhea:RHEA-COMP:10136, Rhea:RHEA-
CC COMP:10137, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:46858,
CC ChEBI:CHEBI:82620, ChEBI:CHEBI:456216; EC=2.7.10.2;
CC -!- ACTIVITY REGULATION: Dephosphorylated and activated by CpsB.
CC {ECO:0000250}.
CC -!- PATHWAY: Capsule biogenesis; capsule polysaccharide biosynthesis.
CC -!- PTM: Autophosphorylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CpsD/CapB family. {ECO:0000305}.
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DR EMBL; AB028896; BAA82278.1; -; Genomic_DNA.
DR EMBL; CP000114; ABA45962.1; -; Genomic_DNA.
DR RefSeq; WP_000197416.1; NC_007432.1.
DR AlphaFoldDB; Q3K0T0; -.
DR SMR; Q3K0T0; -.
DR KEGG; sak:SAK_1259; -.
DR HOGENOM; CLU_052027_2_0_9; -.
DR OMA; MQCIQKT; -.
DR UniPathway; UPA00934; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0045227; P:capsule polysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR025669; AAA_dom.
DR InterPro; IPR005702; EPS_synthesis.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF13614; AAA_31; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR01007; eps_fam; 1.
PE 3: Inferred from homology;
KW ATP-binding; Capsule biogenesis/degradation; Exopolysaccharide synthesis;
KW Kinase; Nucleotide-binding; Phosphoprotein; Transferase;
KW Tyrosine-protein kinase.
FT CHAIN 1..232
FT /note="Tyrosine-protein kinase CpsD"
FT /id="PRO_0000217238"
FT CONFLICT 218..220
FT /note="Missing (in Ref. 1; BAA82278)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 232 AA; 25402 MW; DE4CFA15A9C77C26 CRC64;
MTRLEIVDSK LRQAKKTEEY FNAIRTNIQF SGKENKILAI TSVREGEGKS TTSTSLALSL
AQAGFKTLLI DADTRNSVMS GTFKATGTIK GLTNYLSGNA DLGDIICETN VPRLMVVPSG
KVPPNPTALL QNAYFNKMIE AIKNIFDYII IDTPPIGLVV DAAIIANACD GFILVTQAGR
IKRNYVEKAK EQMEQSGSKF LGIILNKVSE SVATYGDYGD YGNYGKRDRK RK