ID   CPSE_STRA3              Reviewed;         449 AA.
AC   Q04664; Q8VL98; Q8VSS5; Q9JN35;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   17-JAN-2003, sequence version 3.
DT   25-MAY-2022, entry version 115.
DE   RecName: Full=Galactosyl transferase CpsE;
DE            EC=2.7.8.-;
GN   Name=cpsE; OrderedLocusNames=gbs1244;
OS   Streptococcus agalactiae serotype III (strain NEM316).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=211110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=COH1 / Serotype III;
RX   PubMed=8355611; DOI=10.1111/j.1365-2958.1993.tb01631.x;
RA   Rubens C.E., Heggen L.M., Haft R.F., Wessels M.R.;
RT   "Identification of cpsD, a gene essential for type III capsule expression
RT   in group B streptococci.";
RL   Mol. Microbiol. 8:843-855(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SEQUENCE REVISION.
RC   STRAIN=COH1 / Serotype III;
RX   PubMed=10913080; DOI=10.1128/jb.182.16.4466-4477.2000;
RA   Chaffin D.O., Beres S.B., Yim H.H., Rubens C.E.;
RT   "The serotype of type Ia and III group B streptococci is determined by the
RT   polymerase gene within the polycistronic capsule operon.";
RL   J. Bacteriol. 182:4466-4477(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=4-61 / Serotype III, 4-62 / Serotype III, GB00/009 / Serotype III,
RC   GB00/031 / Serotype III, M781 / Serotype III, NCDC SS620 / Serotype III,
RC   SG94/1041 / Serotype III, SG95/545 / Serotype III, SG96/039 / Serotype III,
RC   SG96/585 / Serotype III, SG97/122 / Serotype III, SG98/192 / Serotype III,
RC   SG98/367 / Serotype III, SG99/056 / Serotype III, and
RC   SG99/143 / Serotype III;
RX   PubMed=11773119; DOI=10.1128/jcm.40.1.216-226.2002;
RA   Kong F., Gowan S., Martin D., James G., Gilbert G.L.;
RT   "Serotype identification of Group B Streptococci by PCR and sequencing.";
RL   J. Clin. Microbiol. 40:216-226(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NEM316;
RX   PubMed=12354221; DOI=10.1046/j.1365-2958.2002.03126.x;
RA   Glaser P., Rusniok C., Buchrieser C., Chevalier F., Frangeul L., Msadek T.,
RA   Zouine M., Couve E., Lalioui L., Poyart C., Trieu-Cuot P., Kunst F.;
RT   "Genome sequence of Streptococcus agalactiae, a pathogen causing invasive
RT   neonatal disease.";
RL   Mol. Microbiol. 45:1499-1513(2002).
CC   -!- FUNCTION: Galactosyl transferase is essential for the assembly of the
CC       group B streptococci (GBS) type III capsular polysaccharide. May be
CC       involved in the formation of either or both galactosidic bonds by
CC       catalyzing the addition of galactose to an oligosaccharide precursor or
CC       to a lipid intermediate. Type III capsular polysaccharide consists of a
CC       linear backbone with short side chains ending in residues of N-
CC       acetylneuraminic acid or sialic acid. The presence of sialic acid on
CC       the surface of the organism inhibits activation of the alternative
CC       pathway of complement and is thought to be an important element in the
CC       virulence function of the capsule.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the bacterial sugar transferase family.
CC       {ECO:0000305}.
CC   -!- CAUTION: Was originally called CpsD. {ECO:0000305|PubMed:8355611}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAD46903.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AF163833; AAD53066.1; -; Genomic_DNA.
DR   EMBL; AF332896; AAL57072.1; -; Genomic_DNA.
DR   EMBL; AF332897; AAL57079.1; -; Genomic_DNA.
DR   EMBL; AF332898; AAL57085.1; -; Genomic_DNA.
DR   EMBL; AF332899; AAL57091.1; -; Genomic_DNA.
DR   EMBL; AF332900; AAL57097.1; -; Genomic_DNA.
DR   EMBL; AF363032; AAL56269.1; -; Genomic_DNA.
DR   EMBL; AF363033; AAL56273.1; -; Genomic_DNA.
DR   EMBL; AF363034; AAL56277.1; -; Genomic_DNA.
DR   EMBL; AF363035; AAL56281.1; -; Genomic_DNA.
DR   EMBL; AF363039; AAL56297.1; -; Genomic_DNA.
DR   EMBL; AF363040; AAL56301.1; -; Genomic_DNA.
DR   EMBL; AF363055; AAL56361.1; -; Genomic_DNA.
DR   EMBL; AF363056; AAL56368.1; -; Genomic_DNA.
DR   EMBL; AF381031; AAL57286.1; -; Genomic_DNA.
DR   EMBL; AF367973; AAL56403.1; -; Genomic_DNA.
DR   EMBL; AL766849; CAD46903.1; ALT_INIT; Genomic_DNA.
DR   PIR; T44643; T44643.
DR   AlphaFoldDB; Q04664; -.
DR   SMR; Q04664; -.
DR   STRING; 211110.gbs1243; -.
DR   EnsemblBacteria; CAD46903; CAD46903; CAD46903.
DR   KEGG; san:cpsE; -.
DR   eggNOG; COG2148; Bacteria.
DR   HOGENOM; CLU_024920_3_4_9; -.
DR   Proteomes; UP000000823; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000271; P:polysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR003362; Bact_transf.
DR   InterPro; IPR017475; EPS_sugar_tfrase.
DR   Pfam; PF02397; Bac_transf; 1.
DR   TIGRFAMs; TIGR03025; EPS_sugtrans; 1.
PE   3: Inferred from homology;
KW   Capsule biogenesis/degradation; Cell membrane; Exopolysaccharide synthesis;
KW   Membrane; Transferase; Transmembrane; Transmembrane helix; Virulence.
FT   CHAIN           1..449
FT                   /note="Galactosyl transferase CpsE"
FT                   /id="PRO_0000166463"
FT   TRANSMEM        5..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        27..46
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        59..78
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        88..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        258..280
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   VARIANT         262
FT                   /note="T -> M (in strain: SG96/585 / Serotype III)"
FT   VARIANT         370
FT                   /note="V -> A (in strain: COH1 / Serotype III)"
SQ   SEQUENCE   449 AA;  52378 MW;  A958FE1E0DA01338 CRC64;
     MIQTVVVYFS ASLTLTLITP NFKSNKDLLF VLLIHYIVFY LSDFYRDFWS RGYLEEFKMV
     LKYSFYYIFI SSSLFFIFKN SFTTTRLSFF TFIAMNSILL YLLNSFLKYY RKYSYAKFSR
     DTKVVLITNK DSLSKMTFRN KYDHNYIAVC ILDSSEKDCY DLKHNSLRII NKDALTSELT
     CLTVDQAFIN IPIELFGKYQ IQDIINDIEA MGVIVNVNVE ALSFDNIGEK RIQTFEGYSV
     ITYSMKFYKY SHLIAKRFLD ITGAIIGLLI CGIVAIFLVP QIRKDGGPAI FSQNRVGRNG
     RIFRFYKFRS MRVDAEQIKK DLLVHNQMTG LMFKLEDDPR ITKIGKFIRK TSIDELPQFY
     NVLKGDMSLV GTRPPTVDEY EKYNSTQKRR LSFKPGITGL WQISGRNNIT DFDEIVKLDV
     QYINEWSIWS DIKIILLTLK VVLLGTGAK