ID   CPSE_STRA5              Reviewed;         449 AA.
AC   Q9AFI0;
DT   17-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Galactosyl transferase CpsE;
DE            EC=2.7.8.-;
GN   Name=cpsE; OrderedLocusNames=SAG1171;
OS   Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=208435;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CNCTC 1/82 / Serotype V;
RA   McKinnon K., Chaffin D.O., Rubens C.E.;
RT   "Streptococcus agalactiae type V polysaccharide synthesis operon complete
RT   sequence.";
RL   Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC BAA-23 / CJB 111 / Serotype V, Prague 10/84 / Serotype V,
RC   SG97/489 / Serotype V, SG98/606, and SG99/073 / Serotype V;
RX   PubMed=11773119; DOI=10.1128/jcm.40.1.216-226.2002;
RA   Kong F., Gowan S., Martin D., James G., Gilbert G.L.;
RT   "Serotype identification of Group B Streptococci by PCR and sequencing.";
RL   J. Clin. Microbiol. 40:216-226(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-611 / 2603 V/R;
RX   PubMed=12200547; DOI=10.1073/pnas.182380799;
RA   Tettelin H., Masignani V., Cieslewicz M.J., Eisen J.A., Peterson S.N.,
RA   Wessels M.R., Paulsen I.T., Nelson K.E., Margarit I., Read T.D.,
RA   Madoff L.C., Wolf A.M., Beanan M.J., Brinkac L.M., Daugherty S.C.,
RA   DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Lewis M.R., Radune D.,
RA   Fedorova N.B., Scanlan D., Khouri H.M., Mulligan S., Carty H.A.,
RA   Cline R.T., Van Aken S.E., Gill J., Scarselli M., Mora M., Iacobini E.T.,
RA   Brettoni C., Galli G., Mariani M., Vegni F., Maione D., Rinaudo D.,
RA   Rappuoli R., Telford J.L., Kasper D.L., Grandi G., Fraser C.M.;
RT   "Complete genome sequence and comparative genomic analysis of an emerging
RT   human pathogen, serotype V Streptococcus agalactiae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:12391-12396(2002).
CC   -!- FUNCTION: Galactosyl transferase is essential for the assembly of the
CC       group B streptococci (GBS) type III capsular polysaccharide. May be
CC       involved in the formation of either or both galactosidic bonds by
CC       catalyzing the addition of galactose to an oligosaccharide precursor or
CC       to a lipid intermediate. Type III capsular polysaccharide consists of a
CC       linear backbone with short side chains ending in residues of N-
CC       acetylneuraminic acid or sialic acid. The presence of sialic acid on
CC       the surface of the organism inhibits activation of the alternative
CC       pathway of complement and is thought to be an important element in the
CC       virulence function of the capsule (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the bacterial sugar transferase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN00053.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AF349539; AAK29651.1; -; Genomic_DNA.
DR   EMBL; AF332910; AAL57141.1; -; Genomic_DNA.
DR   EMBL; AF332911; AAL57145.1; -; Genomic_DNA.
DR   EMBL; AF332912; AAL57149.1; -; Genomic_DNA.
DR   EMBL; AF363048; AAL56333.1; -; Genomic_DNA.
DR   EMBL; AF363049; AAL56337.1; -; Genomic_DNA.
DR   EMBL; AE009948; AAN00053.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_688180.1; NC_004116.1.
DR   AlphaFoldDB; Q9AFI0; -.
DR   SMR; Q9AFI0; -.
DR   STRING; 208435.SAG1171; -.
DR   DNASU; 1013978; -.
DR   EnsemblBacteria; AAN00053; AAN00053; SAG1171.
DR   KEGG; sag:SAG1171; -.
DR   PATRIC; fig|208435.3.peg.1177; -.
DR   HOGENOM; CLU_024920_3_4_9; -.
DR   Proteomes; UP000000821; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000271; P:polysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR003362; Bact_transf.
DR   InterPro; IPR017475; EPS_sugar_tfrase.
DR   Pfam; PF02397; Bac_transf; 1.
DR   TIGRFAMs; TIGR03025; EPS_sugtrans; 1.
PE   3: Inferred from homology;
KW   Capsule biogenesis/degradation; Cell membrane; Exopolysaccharide synthesis;
KW   Membrane; Reference proteome; Transferase; Transmembrane;
KW   Transmembrane helix; Virulence.
FT   CHAIN           1..449
FT                   /note="Galactosyl transferase CpsE"
FT                   /id="PRO_0000166464"
FT   TRANSMEM        5..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        27..46
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        59..78
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        88..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        258..280
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   449 AA;  52364 MW;  B9583903BB7B7E8F CRC64;
     MIQTVVVYFS ASLTLTLITP NFKSNKDLLF VLLIHYIVFY LSDFYRDFWS RGYLEEFKMV
     LKYSFYYIFI SSSLFFIFKN SFTTTRLSFF TFIAMNSILL YLLNSFLKYY RKYSYAKFSR
     DTKVVLITNK DSLSKMTFRN KYDHNYIAVC ILDSSEKDCY DLKHNSLRII NKDALTSELT
     CLTVDQAFIN IPIELFGKYQ IQDIINDIEA MGVIVNVNVE ALSFDNIGEK RIQTFEGYSV
     ITYSMKFYKY SHLIAKRFLD ITGAIIGLLI CGIVAIFLVP QIRKDGGPAI FSQNRVGRNG
     RIFRFYKFRS MRVDAEQIKK DLLVHNQMTG LMFKLDDDPR ITKIGKFIRK TSIDELPQFY
     NVLKGDMSLV GTRPPTVDEY EKYNSTQKRR LSFKPGITGL WQISGRNNIT DFDEIVKLDV
     QYINEWSIWS DIKIILLTLK VVLLGTGAK