CPSF1_DANRE
ID CPSF1_DANRE Reviewed; 1451 AA.
AC A0A0R4IC37;
DT 02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2018, sequence version 2.
DT 03-AUG-2022, entry version 32.
DE RecName: Full=Cleavage and polyadenylation specificity factor subunit 1 {ECO:0000250|UniProtKB:Q10570};
DE AltName: Full=Cleavage and polyadenylation specific factor 1 {ECO:0000312|ZFIN:ZDB-GENE-040709-2};
GN Name=cpsf1 {ECO:0000312|ZFIN:ZDB-GENE-040709-2};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=30689892; DOI=10.1093/hmg/ddz029;
RA Ouyang J., Sun W., Xiao X., Li S., Jia X., Zhou L., Wang P., Zhang Q.;
RT "CPSF1 mutations are associated with early-onset high myopia and involved
RT in retinal ganglion cell axon projection.";
RL Hum. Mol. Genet. 28:1959-1970(2019).
CC -!- FUNCTION: Component of the cleavage and polyadenylation specificity
CC factor (CPSF) complex that plays a key role in pre-mRNA 3'-end
CC formation, recognizing the AAUAAA signal sequence and interacting with
CC poly(A) polymerase and other factors to bring about cleavage and
CC poly(A) addition. This subunit is involved in the RNA recognition step
CC of the polyadenylation reaction (By similarity). Plays a role in eye
CC morphogenesis and the development of retinal ganglion cell projections
CC to the tectum (PubMed:30689892). {ECO:0000250|UniProtKB:Q10570,
CC ECO:0000269|PubMed:30689892}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000250|UniProtKB:Q10570}.
CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown results in abnormal eye
CC morphogenesis, small eye size, and reduced number of retinal ganglion
CC cell projections to the tectum. {ECO:0000269|PubMed:30689892}.
CC -!- SIMILARITY: Belongs to the CPSF1 family. {ECO:0000305}.
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DR EMBL; CU467825; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; FP236813; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; FP325126; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; LO018649; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; A0A0R4IC37; -.
DR SMR; A0A0R4IC37; -.
DR STRING; 7955.ENSDARP00000098742; -.
DR Ensembl; ENSDART00000163478; ENSDARP00000130523; ENSDARG00000034178.
DR ZFIN; ZDB-GENE-040709-2; cpsf1.
DR GeneTree; ENSGT00950000183151; -.
DR Reactome; R-DRE-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-DRE-72187; mRNA 3'-end processing.
DR Reactome; R-DRE-73856; RNA Polymerase II Transcription Termination.
DR Reactome; R-DRE-77595; Processing of Intronless Pre-mRNAs.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 19.
DR Bgee; ENSDARG00000034178; Expressed in presomitic mesoderm and 29 other tissues.
DR ExpressionAtlas; A0A0R4IC37; baseline.
DR GO; GO:0005847; C:mRNA cleavage and polyadenylation specificity factor complex; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0060216; P:definitive hemopoiesis; IMP:ZFIN.
DR GO; GO:0006378; P:mRNA polyadenylation; IMP:ZFIN.
DR GO; GO:0031290; P:retinal ganglion cell axon guidance; IMP:ZFIN.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR004871; Cleavage/polyA-sp_fac_asu_C.
DR InterPro; IPR018846; Cleavage/polyA-sp_fac_asu_N.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR Pfam; PF03178; CPSF_A; 1.
DR Pfam; PF10433; MMS1_N; 1.
PE 3: Inferred from homology;
KW Nucleus; Reference proteome.
FT CHAIN 1..1451
FT /note="Cleavage and polyadenylation specificity factor
FT subunit 1"
FT /id="PRO_0000451718"
FT REGION 401..432
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 548..572
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 753..789
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 901..916
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 401..426
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 755..785
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1451 AA; 163195 MW; 54542B0C25BD9FB9 CRC64;
MYAVYRQAHP PTAVEFAVYC NFISSQEKNL VVAGTSQLYV YRIIYDVEST SKSEKSSDGK
SRKEKLEQVA SFSLFGNVMS MASVQLVGTN RDALLLSFKD AKLSVVEYDP GTHDLKTLSL
HYFEEPELRD GFVQNVHIPM VRVDPENRCA VMLVYGTCLV VLPFRKDTLA DEQEGIVGEG
QKSSFLPSYI IDVRELDEKL LNIIDMKFLH GYYEPTLLIL FEPNQTWPGR VAVRQDTCSI
VAISLNIMQK VHPVIWSLSN LPFDCNQVMA VPKPIGGVVV FAVNSLLYLN QSVPPFGVSL
NSLTNGTTAF PLRPQEEVKI TLDCSQASFI TSDKMVISLK GGEIYVLTLI TDGMRSVRAF
HFDKAAASVL TTCMMTMEPG YLFLGSRLGN SLLLRYTEKL QETPMEEGKE NEEKEKQEEP
PNKKKRVDSN WAGCPGKGNL PDELDEIEVY GSEAQSGTQL ATYSFEVCDS ILNIGPCASA
SMGEPAFLSE EFQTNPEPDL EVVVCSGYGK NGALSVLQKS IRPQVVTTFE LPGCHDMWTV
IYCEEKPEKP SAEGDGESPE EEKREPTIED DKKKHGFLIL SREDSTMILQ TGQEIMELDT
SGFATQGPTV YAGNIGDNKY IIQVSPMGIR LLEGVNQLHF IPVDLGSPIV HCSVADPYVV
IMTAEGVVTM FVLKNDSYMG KSHRLALQKP QIHTQSRVIT LCAYRDVSGM FTTENKVSFL
AKEEIAIRTN SETETIIQDI SNTVDDEEEM LYGESNPLTS PNKEESSRGS AAASSAHTGK
ESGSGRQEPS HWCLLVRENG VMEIYQLPDW RLVFLVKNFP VGQRVLVDSS ASQSATQGEL
KKEEVTRQGD IPLVKEVALV SLGYNHSRPY LLAHVEQELL IYEAFPYDQQ QAQSNLKVRF
KKMPHNINYR EKKVKVRKDK KPEGQGEDTL GVKGRVARFR YFQDISGYSG VFICGPSPHW
MLVTSRGAMR LHPMTIDGAI ESFSPFHNIN CPKGFLYFNK QGELRISVLP TYLSYDAPWP
VRKIPLRCTV HYVSYHVESK VYAVCTSVKE PCTRIPRMTG EEKEFETIER DERYIHPQQD
KFSIQLISPV SWEAIPNTRV DLEEWEHVTC MKTVALKSQE TVSGLKGYVA LGTCLMQGEE
VTCRGRILIL DVIEVVPEPG QPLTKNKFKV LYEKEQKGPV TALCHCSGFL VSAIGQKIFL
WSLKDNDLTG MAFIDTQLYI HQMYSIKNFI LAADVMKSIS LLRYQPESKT LSLVSRDAKP
LEVYSIEFMV DNNQLGFLVS DRDKNLMVYM YLPEAKESFG GMRLLRRADF NVGSHVNAFW
RMPCRGTLDT ANKKALTWDN KHITWFATLD GGVGLLLPMQ EKTYRRLLML QNALTTMLPH
HAGLNPKAFR MLHCDRRTLQ NAVKNILDGE LLNKYLYLST MERSELAKKI GTTPDIILDD
LLEIERVTAH F
