CPT2_SOLLC
ID CPT2_SOLLC Reviewed; 314 AA.
AC K7WQ45; A0A3Q7HGZ1;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2013, sequence version 1.
DT 03-AUG-2022, entry version 37.
DE RecName: Full=Nerylneryl diphosphate synthase CPT2, chloroplastic {ECO:0000305};
DE EC=2.5.1.142 {ECO:0000269|PubMed:23134568, ECO:0000269|PubMed:23757397, ECO:0000269|PubMed:25786135};
DE AltName: Full=Cis-prenyltransferase 2 {ECO:0000303|PubMed:23134568};
DE Short=SlCPT2 {ECO:0000303|PubMed:23134568};
DE Flags: Precursor;
GN Name=CPT2 {ECO:0000303|PubMed:23134568};
GN OrderedLocusNames=Solyc08g005660 {ECO:0000305};
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=23757397; DOI=10.1105/tpc.113.111013;
RA Matsuba Y., Nguyen T.T., Wiegert K., Falara V., Gonzales-Vigil E.,
RA Leong B., Schafer P., Kudrna D., Wing R.A., Bolger A.M., Usadel B.,
RA Tissier A., Fernie A.R., Barry C.S., Pichersky E.;
RT "Evolution of a complex locus for terpene biosynthesis in Solanum.";
RL Plant Cell 25:2022-2036(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, COFACTOR,
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=23134568; DOI=10.1111/tpj.12063;
RA Akhtar T.A., Matsuba Y., Schauvinhold I., Yu G., Lees H.A., Klein S.E.,
RA Pichersky E.;
RT "The tomato cis-prenyltransferase gene family.";
RL Plant J. 73:640-652(2013).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Heinz 1706;
RX PubMed=22660326; DOI=10.1038/nature11119;
RG Tomato Genome Consortium;
RT "The tomato genome sequence provides insights into fleshy fruit
RT evolution.";
RL Nature 485:635-641(2012).
RN [4]
RP FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND TISSUE
RP SPECIFICITY.
RX PubMed=25786135; DOI=10.1371/journal.pone.0119302;
RA Matsuba Y., Zi J., Jones A.D., Peters R.J., Pichersky E.;
RT "Biosynthesis of the diterpenoid lycosantalonol via nerylneryl diphosphate
RT in Solanum lycopersicum.";
RL PLoS ONE 10:e0119302-e0119302(2015).
CC -!- FUNCTION: Uses dimethylallyl diphosphate and isopentenyl diphosphate to
CC catalyze the cis-prenyl chain elongation and produce the 20 carbon
CC product nerylneryl diphosphate. {ECO:0000269|PubMed:23134568,
CC ECO:0000269|PubMed:23757397, ECO:0000269|PubMed:25786135}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dimethylallyl diphosphate + 3 isopentenyl diphosphate = 3
CC diphosphate + nerylneryl diphosphate; Xref=Rhea:RHEA:55520,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57623, ChEBI:CHEBI:128769,
CC ChEBI:CHEBI:139032; EC=2.5.1.142;
CC Evidence={ECO:0000269|PubMed:23134568, ECO:0000269|PubMed:23757397,
CC ECO:0000269|PubMed:25786135};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55521;
CC Evidence={ECO:0000269|PubMed:23134568, ECO:0000269|PubMed:23757397,
CC ECO:0000269|PubMed:25786135};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dimethylallyl diphosphate + isopentenyl diphosphate =
CC diphosphate + neryl diphosphate; Xref=Rhea:RHEA:11328,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57623, ChEBI:CHEBI:57665,
CC ChEBI:CHEBI:128769; Evidence={ECO:0000269|PubMed:25786135};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11329;
CC Evidence={ECO:0000269|PubMed:25786135};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=isopentenyl diphosphate + neryl diphosphate = (2Z,6Z)-farnesyl
CC diphosphate + diphosphate; Xref=Rhea:RHEA:64572, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:57665, ChEBI:CHEBI:60374, ChEBI:CHEBI:128769;
CC Evidence={ECO:0000269|PubMed:25786135};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64573;
CC Evidence={ECO:0000269|PubMed:25786135};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2Z,6Z)-farnesyl diphosphate + isopentenyl diphosphate =
CC diphosphate + nerylneryl diphosphate; Xref=Rhea:RHEA:64580,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:60374, ChEBI:CHEBI:128769,
CC ChEBI:CHEBI:139032; Evidence={ECO:0000269|PubMed:25786135};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64581;
CC Evidence={ECO:0000269|PubMed:25786135};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000269|PubMed:23134568};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=3.8 uM for isopentenyl diphosphate {ECO:0000269|PubMed:25786135};
CC KM=22.9 uM for (2Z,6Z)-farnesyl diphosphate
CC {ECO:0000269|PubMed:25786135};
CC pH dependence:
CC Optimum pH is 8.0-8.5. {ECO:0000269|PubMed:25786135};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:23134568}.
CC -!- TISSUE SPECIFICITY: Expressed in stems (PubMed:23134568). Expressed in
CC petiolules (PubMed:25786135). Expressed at low levels in leaf
CC trichomes, old leaf and roots (PubMed:23134568).
CC {ECO:0000269|PubMed:23134568, ECO:0000269|PubMed:25786135}.
CC -!- SIMILARITY: Belongs to the UPP synthase family. {ECO:0000305}.
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DR EMBL; KC807995; AGK82801.1; -; Genomic_DNA.
DR EMBL; JX943884; AFW98426.1; -; Genomic_DNA.
DR EMBL; CM001071; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; K7WQ45; -.
DR SMR; K7WQ45; -.
DR STRING; 4081.Solyc08g005660.1.1; -.
DR KEGG; ag:AFW98426; -.
DR BRENDA; 2.5.1.142; 3101.
DR Proteomes; UP000004994; Chromosome 8.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0047863; F:dimethylallylcistransferase activity; IEA:RHEA.
DR GO; GO:0000287; F:magnesium ion binding; IDA:UniProtKB.
DR GO; GO:0004659; F:prenyltransferase activity; IDA:UniProtKB.
DR CDD; cd00475; Cis_IPPS; 1.
DR Gene3D; 3.40.1180.10; -; 1.
DR HAMAP; MF_01139; ISPT; 1.
DR InterPro; IPR001441; UPP_synth-like.
DR InterPro; IPR018520; UPP_synth-like_CS.
DR InterPro; IPR036424; UPP_synth-like_sf.
DR PANTHER; PTHR10291; PTHR10291; 1.
DR Pfam; PF01255; Prenyltransf; 1.
DR SUPFAM; SSF64005; SSF64005; 1.
DR TIGRFAMs; TIGR00055; uppS; 1.
DR PROSITE; PS01066; UPP_SYNTHASE; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Magnesium; Metal-binding; Plastid; Reference proteome;
KW Transferase; Transit peptide.
FT TRANSIT 1..61
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 62..314
FT /note="Nerylneryl diphosphate synthase CPT2, chloroplastic"
FT /id="PRO_0000450933"
FT ACT_SITE 97
FT /evidence="ECO:0000250|UniProtKB:P60472"
SQ SEQUENCE 314 AA; 36020 MW; 9999116125627C76 CRC64;
MNSSIVSQHF FISLKSSLDL QCWKSSSPSS ISMGEFKGIH DKLQILKLPL TMSDRGLSKI
SCSLSLQTEK LRYDNDDNDD LELHEELIPK HIALIMDGNR RWAKAKGLEV YEGHKLIIPK
LKEICDISSK LGIQVITAFA FSTENWKRSK EEVDFLMQLF EEFFNEFLRF GVRVSVIGCK
SNLPMTLQKC IALTEETTKG NKGLHLVIAL NYGGYYDILQ ATKSIVNKAM NGLLDVEDIN
KNLFEQELES KCPNPDLLIR TGGEQRVSNF LLWQLAYTEF YFTNTLFPDF GEKDLKKAIL
NFQQRHRRFG GHTY
