CPT6_SOLLC
ID CPT6_SOLLC Reviewed; 285 AA.
AC K7W9N9; A0A3Q7GTN3;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 07-OCT-2020, sequence version 2.
DT 03-AUG-2022, entry version 32.
DE RecName: Full=(2Z,6Z)-farnesyl diphosphate synthase CPT6, chloroplastic {ECO:0000305};
DE EC=2.5.1.92 {ECO:0000305};
DE AltName: Full=Cis-prenyltransferase 6 {ECO:0000303|PubMed:23134568};
DE Short=SlCPT6 {ECO:0000303|PubMed:23134568};
DE Flags: Precursor;
GN Name=CPT6 {ECO:0000303|PubMed:23134568};
GN OrderedLocusNames=Solyc06g059990 {ECO:0000305};
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, COFACTOR,
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=23134568; DOI=10.1111/tpj.12063;
RA Akhtar T.A., Matsuba Y., Schauvinhold I., Yu G., Lees H.A., Klein S.E.,
RA Pichersky E.;
RT "The tomato cis-prenyltransferase gene family.";
RL Plant J. 73:640-652(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Heinz 1706;
RX PubMed=22660326; DOI=10.1038/nature11119;
RG Tomato Genome Consortium;
RT "The tomato genome sequence provides insights into fleshy fruit
RT evolution.";
RL Nature 485:635-641(2012).
CC -!- FUNCTION: Uses neryl diphosphate to catalyze the cis-prenyl chain
CC elongation and produce the 15 carbon product (2Z,6Z)-farnesyl
CC diphosphate. {ECO:0000269|PubMed:23134568}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dimethylallyl diphosphate + 2 isopentenyl diphosphate =
CC (2Z,6Z)-farnesyl diphosphate + 2 diphosphate; Xref=Rhea:RHEA:27810,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57623, ChEBI:CHEBI:60374,
CC ChEBI:CHEBI:128769; EC=2.5.1.92;
CC Evidence={ECO:0000269|PubMed:23134568};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:27811;
CC Evidence={ECO:0000269|PubMed:23134568};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dimethylallyl diphosphate + isopentenyl diphosphate =
CC diphosphate + neryl diphosphate; Xref=Rhea:RHEA:11328,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57623, ChEBI:CHEBI:57665,
CC ChEBI:CHEBI:128769; Evidence={ECO:0000269|PubMed:23134568};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11329;
CC Evidence={ECO:0000269|PubMed:23134568};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=isopentenyl diphosphate + neryl diphosphate = (2Z,6Z)-farnesyl
CC diphosphate + diphosphate; Xref=Rhea:RHEA:64572, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:57665, ChEBI:CHEBI:60374, ChEBI:CHEBI:128769;
CC Evidence={ECO:0000269|PubMed:23134568};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64573;
CC Evidence={ECO:0000269|PubMed:23134568};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000269|PubMed:23134568};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:23134568}. Note=Localizes in punctuate patterns
CC inside the chloroplasts. {ECO:0000269|PubMed:23134568}.
CC -!- TISSUE SPECIFICITY: Expressed in roots and red fruits.
CC {ECO:0000269|PubMed:23134568}.
CC -!- SIMILARITY: Belongs to the UPP synthase family. {ECO:0000305}.
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DR EMBL; JX943888; AFW98430.1; -; Genomic_DNA.
DR EMBL; CM001069; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; K7W9N9; -.
DR SMR; K7W9N9; -.
DR STRING; 4081.Solyc06g059990.2.1; -.
DR BRENDA; 2.5.1.92; 3101.
DR Proteomes; UP000004994; Chromosome 6.
DR ExpressionAtlas; K7W9N9; baseline.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0102059; F:2-cis,6-cis-farnesyl pyrophosphate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0047863; F:dimethylallylcistransferase activity; IEA:RHEA.
DR GO; GO:0000287; F:magnesium ion binding; IDA:UniProtKB.
DR GO; GO:0004659; F:prenyltransferase activity; IDA:UniProtKB.
DR CDD; cd00475; Cis_IPPS; 1.
DR Gene3D; 3.40.1180.10; -; 1.
DR HAMAP; MF_01139; ISPT; 1.
DR InterPro; IPR001441; UPP_synth-like.
DR InterPro; IPR018520; UPP_synth-like_CS.
DR InterPro; IPR036424; UPP_synth-like_sf.
DR PANTHER; PTHR10291; PTHR10291; 1.
DR Pfam; PF01255; Prenyltransf; 1.
DR SUPFAM; SSF64005; SSF64005; 1.
DR TIGRFAMs; TIGR00055; uppS; 1.
DR PROSITE; PS01066; UPP_SYNTHASE; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Magnesium; Metal-binding; Plastid; Reference proteome;
KW Transferase; Transit peptide.
FT TRANSIT 1..30
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 31..285
FT /note="(2Z,6Z)-farnesyl diphosphate synthase CPT6,
FT chloroplastic"
FT /id="PRO_0000450937"
FT ACT_SITE 65
FT /evidence="ECO:0000250|UniProtKB:P60472"
FT CONFLICT 118
FT /note="Missing (in Ref. 1; AFW98430)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 285 AA; 32797 MW; 483490FB380801B9 CRC64;
MNSLFVGRPI VKSSYNVYTL PSSICGGHFF KVSNSLSLYD DHRRTRIEII RNSELIPKHV
AIIMDGNRRW AKARGLPVQE GHKFLAPNLK NICNISSKLG IQVITAFAFS TENWNRSSEE
VDFLMRLFEE FFEEFMRLGV RVSLIGGKSK LPTKLQQVIE LTEEVTKSNE GLHLMMALNY
GGQYDMLQAT KNIASKVKDG LIKLEDIDYT LFEQELTTKC AKFPKPDLLI RTGGEQRISN
FLLWQLAYSE LYFTNTLFPD FGEEALMDAI FSFQRRHRRF GGHTY
