ID   CPXP_BRADU              Reviewed;         401 AA.
AC   Q59203;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   28-FEB-2003, sequence version 2.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Cytochrome P450 BJ-1;
DE            EC=1.14.14.-;
DE   AltName: Full=Cytochrome P450 112;
GN   Name=cyp112; OrderedLocusNames=blr2144;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=16349113; DOI=10.1128/aem.59.12.4136-4142.1993;
RA   Tully R.E., Keister D.L.;
RT   "Cloning and mutagenesis of a cytochrome P-450 locus from Bradyrhizobium
RT   japonicum that is expressed anaerobically and symbiotically.";
RL   Appl. Environ. Microbiol. 59:4136-4142(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=9655913; DOI=10.1016/s0167-4781(98)00069-4;
RA   Tully R.E., van Berkum P., Lovins K.W., Keister D.L.;
RT   "Identification and sequencing of a cytochrome P450 gene cluster from
RT   Bradyrhizobium japonicum.";
RL   Biochim. Biophys. Acta 1398:243-255(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: Cytochromes P450 are a group of heme-thiolate monooxygenases.
CC       They oxidize a variety of structurally unrelated compounds, including
CC       steroids, fatty acids, and xenobiotics.
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; U12678; AAC28889.1; -; Genomic_DNA.
DR   EMBL; BA000040; BAC47409.1; -; Genomic_DNA.
DR   PIR; I40208; I40208.
DR   RefSeq; NP_768784.1; NC_004463.1.
DR   RefSeq; WP_011084938.1; NZ_CP011360.1.
DR   AlphaFoldDB; Q59203; -.
DR   SMR; Q59203; -.
DR   STRING; 224911.27350398; -.
DR   EnsemblBacteria; BAC47409; BAC47409; BAC47409.
DR   GeneID; 64067355; -.
DR   KEGG; bja:blr2144; -.
DR   PATRIC; fig|224911.44.peg.1659; -.
DR   eggNOG; COG2124; Bacteria.
DR   HOGENOM; CLU_033716_1_1_5; -.
DR   InParanoid; Q59203; -.
DR   OMA; RDWAGMM; -.
DR   PhylomeDB; Q59203; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR002397; Cyt_P450_B.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00359; BP450.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   3: Inferred from homology;
KW   Heme; Iron; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome.
FT   CHAIN           1..401
FT                   /note="Cytochrome P450 BJ-1"
FT                   /id="PRO_0000052226"
FT   BINDING         350
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        251
FT                   /note="L -> H (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        275
FT                   /note="E -> Q (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   401 AA;  44473 MW;  41DF690D11FDD45A CRC64;
     MSEQQPLPTL PMWRVDHIEP SPEMLALRAN GPIHRVRFPS GHEGWWVTGY DEAKAVLSDA
     AFRPAGMPPA AFTPDSVILG SPGWLVSHEG REHARLRAIV APAFSDRRVK LLVQQVEAIA
     AHLFETLAAQ PQPADLRRHL SFPLPAMVIS ALMGVLYEDH AFFAGLSDEV MTHQHESGPR
     SASRLAWEEL RAYIRGKMRD KRQDPDDNLL TDLLAAVDQG KASEEEAVGL AAGMLVAGHE
     STVAQIEFGL LAMFRHPQQR ERLVGDPSLV DKAVEEILRM YPPGAGWDGI MRYPRTDVTI
     AGEHIPAESK VLVGLPATSF DPHHFDDPEI FDIERQEKPH LAFSYGPHAC IGVALARLEL
     KVVFGSIFQR LPALRLAVAP EQLKLRKEII TGGFEQFPVL W