ID   CPXR_BRADU              Reviewed;         429 AA.
AC   Q59204;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=Cytochrome P450 BJ-3;
DE            EC=1.14.14.-;
DE   AltName: Full=Cytochrome P450 114;
GN   Name=cyp114; OrderedLocusNames=blr2145;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=16349113; DOI=10.1128/aem.59.12.4136-4142.1993;
RA   Tully R.E., Keister D.L.;
RT   "Cloning and mutagenesis of a cytochrome P-450 locus from Bradyrhizobium
RT   japonicum that is expressed anaerobically and symbiotically.";
RL   Appl. Environ. Microbiol. 59:4136-4142(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=9655913; DOI=10.1016/s0167-4781(98)00069-4;
RA   Tully R.E., van Berkum P., Lovins K.W., Keister D.L.;
RT   "Identification and sequencing of a cytochrome P450 gene cluster from
RT   Bradyrhizobium japonicum.";
RL   Biochim. Biophys. Acta 1398:243-255(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: Cytochromes P450 are a group of heme-thiolate monooxygenases.
CC       They oxidize a variety of structurally unrelated compounds, including
CC       steroids, fatty acids, and xenobiotics.
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC28890.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; U12678; AAC28890.1; ALT_INIT; Genomic_DNA.
DR   EMBL; BA000040; BAC47410.1; -; Genomic_DNA.
DR   PIR; I40209; I40209.
DR   RefSeq; NP_768785.1; NC_004463.1.
DR   RefSeq; WP_011084939.1; NZ_CP011360.1.
DR   AlphaFoldDB; Q59204; -.
DR   SMR; Q59204; -.
DR   STRING; 224911.27350399; -.
DR   EnsemblBacteria; BAC47410; BAC47410; BAC47410.
DR   GeneID; 64021893; -.
DR   KEGG; bja:blr2145; -.
DR   PATRIC; fig|224911.44.peg.1660; -.
DR   eggNOG; COG2124; Bacteria.
DR   HOGENOM; CLU_033716_1_0_5; -.
DR   InParanoid; Q59204; -.
DR   OMA; CDFARDV; -.
DR   PhylomeDB; Q59204; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0036199; F:cholest-4-en-3-one 26-monooxygenase activity; IBA:GO_Central.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0008395; F:steroid hydroxylase activity; IBA:GO_Central.
DR   GO; GO:0006707; P:cholesterol catabolic process; IBA:GO_Central.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR002397; Cyt_P450_B.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00359; BP450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   3: Inferred from homology;
KW   Heme; Iron; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome.
FT   CHAIN           1..429
FT                   /note="Cytochrome P450 BJ-3"
FT                   /id="PRO_0000052227"
FT   BINDING         376
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   429 AA;  46921 MW;  B4CC331406007983 CRC64;
     MDVQETTAAC RDAFAELASP ACIHDPYPFM RWLREHDPVH RAASGLFLLS RHADIFWAFK
     ATGDAFRGPA PGELARYFSR AATSPSLNLL ASTLAMKDPP THTRLRRLIS RDFTMGQIDN
     LRPSIARIVA ARLDGITPAL ERGEAVDLHR EFALALPMLV FAELFGMPQD DMFELAAGIG
     TILEGLGPHA SDPQLAAADA ASARVQAYFG DLIQRKRTDP RRDIVSMLVG AHDDDADTLS
     DAELISMLWG MLLGGFVTTA ASIDHAVLAM LAYPEQRHWL QADAARVRAF VEEVLRCDAP
     AMFSSIPRIA QRDIELGGVV IPKNADVRVL IASGNRDPDA FADPDRFDPA RFYGTSPGMS
     TDGKIMLSFG HGIHFCLGAQ LARVQLAESL PRIQARFPTL AFAGQPTREP SAFLRTFRTL
     PVRLHAQGS