ID   CPXU_BRADU              Reviewed;         447 AA.
AC   Q59205;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   28-FEB-2003, sequence version 2.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Cytochrome P450 BJ-4;
DE            EC=1.14.14.-;
DE   AltName: Full=Cytochrome P450 117;
GN   Name=cyp117; OrderedLocusNames=blr2147;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=16349113; DOI=10.1128/aem.59.12.4136-4142.1993;
RA   Tully R.E., Keister D.L.;
RT   "Cloning and mutagenesis of a cytochrome P-450 locus from Bradyrhizobium
RT   japonicum that is expressed anaerobically and symbiotically.";
RL   Appl. Environ. Microbiol. 59:4136-4142(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=9655913; DOI=10.1016/s0167-4781(98)00069-4;
RA   Tully R.E., van Berkum P., Lovins K.W., Keister D.L.;
RT   "Identification and sequencing of a cytochrome P450 gene cluster from
RT   Bradyrhizobium japonicum.";
RL   Biochim. Biophys. Acta 1398:243-255(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: Cytochromes P450 are a group of heme-thiolate monooxygenases.
CC       They oxidize a variety of structurally unrelated compounds, including
CC       steroids, fatty acids, and xenobiotics.
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC28893.1; Type=Frameshift; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U12678; AAC28893.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; BA000040; BAC47412.1; -; Genomic_DNA.
DR   PIR; I40212; I40212.
DR   RefSeq; NP_768787.1; NC_004463.1.
DR   RefSeq; WP_011084941.1; NZ_CP011360.1.
DR   AlphaFoldDB; Q59205; -.
DR   SMR; Q59205; -.
DR   STRING; 224911.27350401; -.
DR   EnsemblBacteria; BAC47412; BAC47412; BAC47412.
DR   GeneID; 64021896; -.
DR   KEGG; bja:blr2147; -.
DR   PATRIC; fig|224911.44.peg.1663; -.
DR   eggNOG; COG2124; Bacteria.
DR   HOGENOM; CLU_001570_5_1_5; -.
DR   InParanoid; Q59205; -.
DR   OMA; RDRMYAM; -.
DR   PhylomeDB; Q59205; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0016125; P:sterol metabolic process; IBA:GO_Central.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002403; Cyt_P450_E_grp-IV.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00465; EP450IV.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   3: Inferred from homology;
KW   Heme; Iron; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome.
FT   CHAIN           1..447
FT                   /note="Cytochrome P450 BJ-4"
FT                   /id="PRO_0000052228"
FT   BINDING         392
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   447 AA;  50011 MW;  7BD4D3CDD3A88061 CRC64;
     MDMLLNPLNR RHRLRHDIPV VPGAFPLVGH LPAVVCDLPR LLRRAERTLG SHFWLDFGPA
     GHLMTSLDPD ALALLRHKDV SSGLIEDIAP ELFGGTLVAQ DGIAHRQARD AIQAALLPKG
     LTLAGIGELF APVIRARVQR WRERGDVTIL RETGDLMLKL IFSLMGIPAQ DLPGWHRKYR
     QLLQLIVAPP VDLPGLPLRR GRAARDWIDA RLREFVRAAR EHASRTGLIN DMVSAFDRSD
     DALSDDVLVA NIRLLLLGGH DTTASTMAWM VIELARQPGL WDALVEEAQR VGAVPTRHAD
     LAQCPVAEAL FRETLRVHPA TPLLVRRALR ELRIGQQRIP TGTDLCIPLL HFSTSALLHE
     APDQFRLARW LQRTEPIRPV DMLQFGTGPH FCMGYHLVWL ELVQFCIALA LTMHEAGVRP
     RLLSGVEKGR RYYPTAHPSM TIRIGFS