ID   CPY1_CONFR              Reviewed;          69 AA.
AC   B3SVF1;
DT   31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   02-SEP-2008, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Conopeptide Y-Fe1;
DE   AltName: Full=CPY-Fe1;
DE   Flags: Precursor;
OS   Conus ferrugineus (Cone snail).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Strategoconus.
OX   NCBI_TaxID=379542;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 40-69, SYNTHESIS OF 40-69,
RP   FUNCTION, BIOASSAY, TOXIC DOSE, AND MASS SPECTROMETRY.
RC   TISSUE=Venom, and Venom duct;
RX   PubMed=18505731; DOI=10.1074/jbc.m800084200;
RA   Imperial J.S., Chen P., Sporning A., Terlau H., Daly N.L., Craik D.J.,
RA   Alewood P.F., Olivera B.M.;
RT   "Tyrosine-rich conopeptides affect voltage-gated K+ channels.";
RL   J. Biol. Chem. 283:23026-23032(2008).
CC   -!- FUNCTION: Tyrosine-rich conopeptide that specifically targets voltage-
CC       gated potassium channel Kv1.6/KCNA6 (IC(50) is 8.8 uM) that is
CC       expressed in Xenopus oocytes. In vivo, causes seizures (at 5 nmol) and
CC       death (20 nmol) when intracranially injected into mice, and causes
CC       paralysis (at 10 pmol) to C.elegans. {ECO:0000269|PubMed:18505731}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC   -!- MASS SPECTROMETRY: Mass=4040.1; Method=Unknown;
CC       Evidence={ECO:0000269|PubMed:18505731};
CC   -!- TOXIC DOSE: Dose that causes mild to moderate seizure (ED(50)) is 5
CC       nmol when intracranially injected into mice. With 20 nmol injected per
CC       mouse, most of the mice developed severe seizures and died.
CC       {ECO:0000269|PubMed:18505731}.
CC   -!- MISCELLANEOUS: The mature peptide does not contain cysteine residues.
CC   -!- MISCELLANEOUS: Does not inhibit Kv1.2/KCNA2 (IC(50) is >30 uM),
CC       Kv1.3/KCNA3 (IC(50) is >50 uM), Kv1.4/KCNA4 (IC(50) is >50 uM),
CC       Kv1.5/KCNA5 (IC(50) is >50 uM). Seems also ineffective on Kv1.1/KCNA2,
CC       Kv2.1/KCNB1, Kv3.4/KCNC4, and Nav1.2/SCN2A (PubMed:18505731).
CC       {ECO:0000305|PubMed:18505731}.
CC   -!- SIMILARITY: Belongs to the conotoxin M superfamily. Conopeptide Y
CC       family. {ECO:0000305}.
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DR   EMBL; EU000529; ABV74050.1; -; mRNA.
DR   AlphaFoldDB; B3SVF1; -.
DR   PRIDE; B3SVF1; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004214; Conotoxin.
DR   Pfam; PF02950; Conotoxin; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Ion channel impairing toxin; Neurotoxin;
KW   Potassium channel impairing toxin; Secreted; Signal; Toxin;
KW   Voltage-gated potassium channel impairing toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..69
FT                   /id="PRO_5000379124"
FT   PEPTIDE         40..69
FT                   /note="Conopeptide Y-Fe1"
FT                   /id="PRO_5000379125"
SQ   SEQUENCE   69 AA;  8184 MW;  C37D93E42331AC9F CRC64;
     MSKLGVVLFV FLLLLPLAAP QPVGDQPADQ PADRNAEARG TYLYPFSYYR LWRYFTRFLH
     KQPYYYVHI