2SS3_BRANA
ID 2SS3_BRANA Reviewed; 125 AA.
AC P80208;
DT 13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 60.
DE RecName: Full=Napin-3;
DE AltName: Full=1.7S seed storage protein;
DE AltName: Full=Napin BnIII;
DE AltName: Full=Napin nIII;
DE Contains:
DE RecName: Full=Napin-3 small chain;
DE Contains:
DE RecName: Full=Napin-3 large chain;
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708;
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE, SUBUNIT, AND ALLERGEN.
RC TISSUE=Seed {ECO:0000269|PubMed:9249277};
RX PubMed=9249277; DOI=10.1046/j.1365-2222.1997.660843.x;
RA Monsalve R.I., Gonzalez de la Pena M.A., Lopez-Otin C., Fiandor A.,
RA Fernandez C., Villalba M., Rodriguez R.;
RT "Detection, isolation and complete amino acid sequence of an aeroallergenic
RT protein from rapeseed flour.";
RL Clin. Exp. Allergy 27:833-841(1997).
RN [2] {ECO:0000312|PIR:S16183}
RP PROTEIN SEQUENCE OF 1-37, AND SUBUNIT.
RC TISSUE=Seed {ECO:0000269|PubMed:2065094};
RX PubMed=2065094; DOI=10.1016/0167-4838(91)90568-k;
RA Monsalve R.I., Villalba M., Lopez-Otin C., Rodriguez R.;
RT "Structural analysis of the small chain of the 2S albumin, napin nIII, from
RT rapeseed. Chemical and spectroscopic evidence of an intramolecular bond
RT formation.";
RL Biochim. Biophys. Acta 1078:265-272(1991).
CC -!- FUNCTION: The small, basic, water-soluble napins are one of the two
CC major kinds of storage proteins synthesized in the seed during its
CC maturation. {ECO:0000305}.
CC -!- SUBUNIT: The mature protein consists of a small and a large chain
CC linked by disulfide bonds. {ECO:0000269|PubMed:2065094,
CC ECO:0000269|PubMed:9249277}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE.
CC {ECO:0000269|PubMed:9249277}.
CC -!- SIMILARITY: Belongs to the 2S seed storage albumins family.
CC {ECO:0000255}.
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DR PIR; S16183; S16183.
DR AlphaFoldDB; P80208; -.
DR SMR; P80208; -.
DR Allergome; 169; Bra n 1.
DR Allergome; 3162; Bra n 1.0101.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR CDD; cd00261; AAI_SS; 1.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR044723; AAI_SS_dom.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR InterPro; IPR000617; Napin/2SS/CON.
DR PANTHER; PTHR35496; PTHR35496; 2.
DR Pfam; PF00234; Tryp_alpha_amyl; 1.
DR PRINTS; PR00496; NAPIN.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
PE 1: Evidence at protein level;
KW Allergen; Direct protein sequencing; Disulfide bond; Seed storage protein;
KW Storage protein.
FT CHAIN 1..37
FT /note="Napin-3 small chain"
FT /id="PRO_0000239459"
FT CHAIN 38..125
FT /note="Napin-3 large chain"
FT /id="PRO_0000239460"
FT DISULFID 10..62
FT /note="Interchain (between small and large chains)"
FT /evidence="ECO:0000250|UniProtKB:P24565"
FT DISULFID 23..51
FT /note="Interchain (between small and large chains)"
FT /evidence="ECO:0000250|UniProtKB:P24565"
FT DISULFID 52..107
FT /evidence="ECO:0000250|UniProtKB:P24565"
FT DISULFID 64..115
FT /evidence="ECO:0000250|UniProtKB:P24565"
FT NON_CONS 37..38
FT /evidence="ECO:0000303|PubMed:9249277"
SQ SEQUENCE 125 AA; 14035 MW; C4042A1BF07099E8 CRC64;
SAGPFRIPKC RKEFQQAQHL RACQQWLHKQ AMQSGSGPQG PQQRPPLLQQ CCNELHQEEP
LCVCPTLKGA SRAVKQQVRQ QQGQQGQQLQ QVISRIYQTA THLPKVCNIP QVSVCPFQKT
MPGPS
