CR1AA_BACTK
ID CR1AA_BACTK Reviewed; 1176 AA.
AC P0A366; P02965; P09664; P09665; P16478; Q9RED5;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 70.
DE RecName: Full=Pesticidal crystal protein Cry1Aa;
DE AltName: Full=133 kDa crystal protein;
DE AltName: Full=Crystaline entomocidal protoxin;
DE AltName: Full=Insecticidal delta-endotoxin CryIA(a);
GN Name=cry1Aa; Synonyms=cry-1-1, cry1A(a), cryA, crybns3-1, cryIA(a), icp;
OS Bacillus thuringiensis subsp. kurstaki.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=29339;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=HD-1;
RX PubMed=2581950; DOI=10.1016/s0021-9258(18)88966-9;
RA Schnepf H.E., Wong H.C., Whiteley H.R.;
RT "The amino acid sequence of a crystal protein from Bacillus thuringiensis
RT deduced from the DNA base sequence.";
RL J. Biol. Chem. 260:6264-6272(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=BNS3;
RA Tounsi S., J'Mal A., Zouari N., Jaoua S.;
RT "Cloning and nucleotide sequence of a novel cry1Aa-type gene from Bacillus
RT thuringiensis subsp.kurstaki.";
RL Biotechnol. Lett. 21:771-775(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-333, AND PROTEIN SEQUENCE OF 1-9.
RC STRAIN=HD-1;
RX PubMed=6296116; DOI=10.1016/s0021-9258(18)33082-5;
RA Wong H.C., Schnepf H.E., Whiteley H.R.;
RT "Transcriptional and translational start sites for the Bacillus
RT thuringiensis crystal protein gene.";
RL J. Biol. Chem. 258:1960-1967(1983).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 33-609.
RC STRAIN=HD-1;
RX PubMed=7490762; DOI=10.1006/jmbi.1995.0630;
RA Grochulski P., Masson L., Borisova S., Pusztai-Carey M., Schwartz J.L.,
RA Brousseau R., Cygler M.;
RT "Bacillus thuringiensis CryIA(a) insecticidal toxin: crystal structure and
RT channel formation.";
RL J. Mol. Biol. 254:447-464(1995).
CC -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC epithelial cells of many lepidopteran larvae.
CC -!- INTERACTION:
CC P0A366; Q9XY09: btr175; Xeno; NbExp=10; IntAct=EBI-7210432, EBI-7210462;
CC -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC and is accumulated both as an inclusion and as part of the spore coat.
CC -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC terminus.
CC -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR EMBL; M11250; AAA22353.1; -; Genomic_DNA.
DR EMBL; Y09663; CAA70856.1; -; mRNA.
DR EMBL; J01554; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A22617; A22617.
DR PDB; 1CIY; X-ray; 2.25 A; A=29-618.
DR PDBsum; 1CIY; -.
DR AlphaFoldDB; P0A366; -.
DR SMR; P0A366; -.
DR IntAct; P0A366; 1.
DR MINT; P0A366; -.
DR EvolutionaryTrace; P0A366; -.
DR GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 1.20.190.10; -; 1.
DR Gene3D; 2.100.10.10; -; 1.
DR InterPro; IPR041587; Cry_V.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR038979; Pest_crys.
DR InterPro; IPR005638; Pest_crys_C.
DR InterPro; IPR005639; Pest_crys_N.
DR InterPro; IPR036716; Pest_crys_N_sf.
DR InterPro; IPR001178; Pest_cryst_cen_dom.
DR InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR PANTHER; PTHR37003; PTHR37003; 1.
DR Pfam; PF17997; Cry1Ac_D5; 1.
DR Pfam; PF03944; Endotoxin_C; 1.
DR Pfam; PF00555; Endotoxin_M; 1.
DR Pfam; PF03945; Endotoxin_N; 1.
DR SUPFAM; SSF49785; SSF49785; 1.
DR SUPFAM; SSF51096; SSF51096; 1.
DR SUPFAM; SSF56849; SSF56849; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Sporulation; Toxin; Virulence.
FT CHAIN 1..1176
FT /note="Pesticidal crystal protein Cry1Aa"
FT /id="PRO_0000174019"
FT VARIANT 77
FT /note="P -> L (in strain: BNS3)"
FT CONFLICT 1009
FT /note="V -> L (in Ref. 1; AAA22353)"
FT /evidence="ECO:0000305"
FT HELIX 35..48
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 51..53
FT /evidence="ECO:0007829|PDB:1CIY"
FT HELIX 54..63
FT /evidence="ECO:0007829|PDB:1CIY"
FT HELIX 70..84
FT /evidence="ECO:0007829|PDB:1CIY"
FT HELIX 90..119
FT /evidence="ECO:0007829|PDB:1CIY"
FT HELIX 124..144
FT /evidence="ECO:0007829|PDB:1CIY"
FT HELIX 145..148
FT /evidence="ECO:0007829|PDB:1CIY"
FT HELIX 154..178
FT /evidence="ECO:0007829|PDB:1CIY"
FT HELIX 180..182
FT /evidence="ECO:0007829|PDB:1CIY"
FT HELIX 186..218
FT /evidence="ECO:0007829|PDB:1CIY"
FT HELIX 223..239
FT /evidence="ECO:0007829|PDB:1CIY"
FT HELIX 241..244
FT /evidence="ECO:0007829|PDB:1CIY"
FT HELIX 245..250
FT /evidence="ECO:0007829|PDB:1CIY"
FT TURN 252..254
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 266..269
FT /evidence="ECO:0007829|PDB:1CIY"
FT HELIX 271..274
FT /evidence="ECO:0007829|PDB:1CIY"
FT HELIX 284..289
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 298..310
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 313..325
FT /evidence="ECO:0007829|PDB:1CIY"
FT HELIX 326..328
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 344..351
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 357..367
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 380..390
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 393..395
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 400..403
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 407..409
FT /evidence="ECO:0007829|PDB:1CIY"
FT HELIX 410..412
FT /evidence="ECO:0007829|PDB:1CIY"
FT HELIX 423..426
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 429..434
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 444..449
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 452..456
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 467..474
FT /evidence="ECO:0007829|PDB:1CIY"
FT HELIX 475..477
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 479..481
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 486..488
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 492..496
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 498..514
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 522..532
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 534..540
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 543..550
FT /evidence="ECO:0007829|PDB:1CIY"
FT HELIX 562..564
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 566..569
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 577..590
FT /evidence="ECO:0007829|PDB:1CIY"
FT STRAND 596..605
FT /evidence="ECO:0007829|PDB:1CIY"
SQ SEQUENCE 1176 AA; 133120 MW; E2EE15AF12E5DD85 CRC64;
MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF VPGAGFVLGL
VDIIWGIFGP SQWDAFPVQI EQLINQRIEE FARNQAISRL EGLSNLYQIY AESFREWEAD
PTNPALREEM RIQFNDMNSA LTTAIPLLAV QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ
RWGFDAATIN SRYNDLTRLI GNYTDYAVRW YNTGLERVWG PDSRDWVRYN QFRRELTLTV
LDIVALFSNY DSRRYPIRTV SQLTREIYTN PVLENFDGSF RGMAQRIEQN IRQPHLMDIL
NSITIYTDVH RGFNYWSGHQ ITASPVGFSG PEFAFPLFGN AGNAAPPVLV SLTGLGIFRT
LSSPLYRRII LGSGPNNQEL FVLDGTEFSF ASLTTNLPST IYRQRGTVDS LDVIPPQDNS
VPPRAGFSHR LSHVTMLSQA AGAVYTLRAP TFSWQHRSAE FNNIIPSSQI TQIPLTKSTN
LGSGTSVVKG PGFTGGDILR RTSPGQISTL RVNITAPLSQ RYRVRIRYAS TTNLQFHTSI
DGRPINQGNF SATMSSGSNL QSGSFRTVGF TTPFNFSNGS SVFTLSAHVF NSGNEVYIDR
IEFVPAEVTF EAEYDLERAQ KAVNELFTSS NQIGLKTDVT DYHIDQVSNL VECLSDEFCL
DEKQELSEKV KHAKRLSDER NLLQDPNFRG INRQLDRGWR GSTDITIQGG DDVFKENYVT
LLGTFDECYP TYLYQKIDES KLKAYTRYQL RGYIEDSQDL EIYLIRYNAK HETVNVPGTG
SLWPLSAQSP IGKCGEPNRC APHLEWNPDL DCSCRDGEKC AHHSHHFSLD IDVGCTDLNE
DLGVWVIFKI KTQDGHARLG NLEFLEEKPL VGEALARVKR AEKKWRDKRE KLEWETNIVY
KEAKESVDAL FVNSQYDQLQ ADTNIAMIHA ADKRVHSIRE AYLPELSVIP GVNAAIFEEL
EGRIFTAFSL YDARNVIKNG DFNNGLSCWN VKGHVDVEEQ NNQRSVLVVP EWEAEVSQEV
RVCPGRGYIL RVTAYKEGYG EGCVTIHEIE NNTDELKFSN CVEEEIYPNN TVTCNDYTVN
QEEYGGAYTS RNRGYNEAPS VPADYASVYE EKSYTDGRRE NPCEFNRGYR DYTPLPVGYV
TKELEYFPET DKVWIEIGET EGTFIVDSVE LLLMEE
