CR1AB_BACTA
ID CR1AB_BACTA Reviewed; 1155 AA.
AC P0A372; P06577; P06578; P09663; P09666; P09667; P21257; Q45789;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1988, sequence version 1.
DT 25-MAY-2022, entry version 62.
DE RecName: Full=Pesticidal crystal protein Cry1Ab;
DE AltName: Full=130 kDa crystal protein;
DE AltName: Full=Crystaline entomocidal protoxin;
DE AltName: Full=Insecticidal delta-endotoxin CryIA(b);
GN Name=cry1Ab; Synonyms=bt2, cry-1-2, cry1A(b), cryIA(b), cryIC1;
OS Bacillus thuringiensis subsp. aizawai.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=1433;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=IPL7;
RX PubMed=3297927; DOI=10.1016/0378-1119(87)90098-9;
RA Oeda K., Oshie K., Shimizu M., Nakamura K., Yamamoto H., Nakayama I.,
RA Ohkawa H.;
RT "Nucleotide sequence of the insecticidal protein gene of Bacillus
RT thuringiensis strain aizawai IPL7 and its high-level expression in
RT Escherichia coli.";
RL Gene 53:113-119(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=IC1;
RX PubMed=3205732; DOI=10.1093/nar/16.22.10927;
RA Haider M.Z., Ellar D.J.;
RT "Nucleotide sequence of a Bacillus thuringiensis aizawai IC1 entomocidal
RT crystal protein gene.";
RL Nucleic Acids Res. 16:10927-10927(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=HD-133;
RX PubMed=8234543;
RA Chak K.-F., Jen J.C.;
RT "Complete nucleotide sequence and identification of a putative promoter
RT region for the expression in Escherichia coli of the cryIA(b) gene from
RT Bacillus thuringiensis var. aizawai HD133.";
RL Proc. Natl. Sci. Counc. Repub. China, B, Life Sci. 17:7-14(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 429-725.
RC STRAIN=IC1;
RX PubMed=2769751; DOI=10.1016/0022-2836(89)90094-6;
RA Haider M.Z., Ellar D.J.;
RT "Functional mapping of an entomocidal delta-endotoxin. Single amino acid
RT changes produced by site-directed mutagenesis influence toxicity and
RT specificity of the protein.";
RL J. Mol. Biol. 208:183-194(1989).
CC -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC epithelial cells of many lepidopteran larvae.
CC -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC and is accumulated both as an inclusion and as part of the spore coat.
CC -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC terminus.
CC -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR EMBL; M16463; AAA22551.1; -; Genomic_DNA.
DR EMBL; X13233; CAA31620.1; -; Genomic_DNA.
DR EMBL; X54939; CAA38701.1; -; Genomic_DNA.
DR EMBL; X16315; CAA34382.1; -; Genomic_DNA.
DR PIR; A26513; A26513.
DR PIR; S02134; S02134.
DR PDB; 6DJ4; X-ray; 3.01 A; A=29-476.
DR PDBsum; 6DJ4; -.
DR AlphaFoldDB; P0A372; -.
DR SMR; P0A372; -.
DR GeneID; 67470639; -.
DR GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 1.20.190.10; -; 1.
DR Gene3D; 2.100.10.10; -; 1.
DR InterPro; IPR041587; Cry_V.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR038979; Pest_crys.
DR InterPro; IPR005638; Pest_crys_C.
DR InterPro; IPR005639; Pest_crys_N.
DR InterPro; IPR036716; Pest_crys_N_sf.
DR InterPro; IPR001178; Pest_cryst_cen_dom.
DR InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR PANTHER; PTHR37003; PTHR37003; 1.
DR Pfam; PF17997; Cry1Ac_D5; 1.
DR Pfam; PF03944; Endotoxin_C; 1.
DR Pfam; PF00555; Endotoxin_M; 1.
DR Pfam; PF03945; Endotoxin_N; 1.
DR SUPFAM; SSF49785; SSF49785; 1.
DR SUPFAM; SSF51096; SSF51096; 1.
DR SUPFAM; SSF56849; SSF56849; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Sporulation; Toxin; Virulence.
FT CHAIN 1..1155
FT /note="Pesticidal crystal protein Cry1Ab"
FT /id="PRO_0000174021"
FT CONFLICT 282..283
FT /note="GS -> AL (in Ref. 1; AAA22551)"
FT /evidence="ECO:0000305"
FT CONFLICT 450
FT /note="A -> P (in Ref. 2; CAA31620)"
FT /evidence="ECO:0000305"
FT CONFLICT 537
FT /note="F -> L (in Ref. 2 and 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 545
FT /note="P -> I (in Ref. 2 and 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 568
FT /note="T -> I (in Ref. 2 and 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 731
FT /note="P -> L (in Ref. 2; CAA31620)"
FT /evidence="ECO:0000305"
FT CONFLICT 785
FT /note="P -> R (in Ref. 2; CAA31620)"
FT /evidence="ECO:0000305"
FT CONFLICT 978
FT /note="H -> Q (in Ref. 1; AAA22551)"
FT /evidence="ECO:0000305"
FT CONFLICT 1036
FT /note="C -> F (in Ref. 1; AAA22551)"
FT /evidence="ECO:0000305"
FT HELIX 35..47
FT /evidence="ECO:0007829|PDB:6DJ4"
FT HELIX 54..63
FT /evidence="ECO:0007829|PDB:6DJ4"
FT TURN 64..66
FT /evidence="ECO:0007829|PDB:6DJ4"
FT HELIX 71..84
FT /evidence="ECO:0007829|PDB:6DJ4"
FT HELIX 90..119
FT /evidence="ECO:0007829|PDB:6DJ4"
FT HELIX 124..144
FT /evidence="ECO:0007829|PDB:6DJ4"
FT HELIX 145..148
FT /evidence="ECO:0007829|PDB:6DJ4"
FT TURN 154..157
FT /evidence="ECO:0007829|PDB:6DJ4"
FT HELIX 158..182
FT /evidence="ECO:0007829|PDB:6DJ4"
FT HELIX 186..218
FT /evidence="ECO:0007829|PDB:6DJ4"
FT HELIX 223..239
FT /evidence="ECO:0007829|PDB:6DJ4"
FT HELIX 241..244
FT /evidence="ECO:0007829|PDB:6DJ4"
FT HELIX 245..250
FT /evidence="ECO:0007829|PDB:6DJ4"
FT TURN 252..254
FT /evidence="ECO:0007829|PDB:6DJ4"
FT STRAND 266..269
FT /evidence="ECO:0007829|PDB:6DJ4"
FT HELIX 271..275
FT /evidence="ECO:0007829|PDB:6DJ4"
FT HELIX 284..289
FT /evidence="ECO:0007829|PDB:6DJ4"
FT STRAND 298..312
FT /evidence="ECO:0007829|PDB:6DJ4"
FT STRAND 314..326
FT /evidence="ECO:0007829|PDB:6DJ4"
FT STRAND 343..345
FT /evidence="ECO:0007829|PDB:6DJ4"
FT STRAND 358..368
FT /evidence="ECO:0007829|PDB:6DJ4"
FT STRAND 379..390
FT /evidence="ECO:0007829|PDB:6DJ4"
FT STRAND 399..403
FT /evidence="ECO:0007829|PDB:6DJ4"
FT STRAND 406..408
FT /evidence="ECO:0007829|PDB:6DJ4"
FT HELIX 409..411
FT /evidence="ECO:0007829|PDB:6DJ4"
FT TURN 422..424
FT /evidence="ECO:0007829|PDB:6DJ4"
FT STRAND 427..438
FT /evidence="ECO:0007829|PDB:6DJ4"
FT TURN 441..443
FT /evidence="ECO:0007829|PDB:6DJ4"
FT STRAND 445..457
FT /evidence="ECO:0007829|PDB:6DJ4"
FT STRAND 468..475
FT /evidence="ECO:0007829|PDB:6DJ4"
SQ SEQUENCE 1155 AA; 130624 MW; 43461A64C7AC7CAF CRC64;
MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF VPGAGFVLGL
VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL EGLSNLYQIY AESFREWEAD
PTNPALREEM RIQFNDMNSA LTTAIPLFAV QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ
RWGFDAATIN SRYNDLTRLI GNYTDHAVRW YNTGLERVWG PDSRDWIRYN QFRRELTLTV
LDIVSLFPNY DSRTYPIRTV SQLTREIYTN PVLENFDGSF RGSAQGIEGS IRSPHLMDIL
NSITIYTDAH RGEYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI VAQLGQGVYR
TLSSTLYRRP FNIGINNQQL SVLDGTEFAY GTSSNLPSAV YRKSGTVDSL DEIPPQNNNV
PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA PMFSWIHRSA EFNNIIPSSQ ITQIPLTKST
NLGSGTSVVK GPGFTGGDIL RRTSPGQIST LRVNITAPLS QRYRVRIRYA STTNLQFHTS
IDGRPINQGN FSATMSSGSN LQSGSFRTVG FTTPFNFSNG SSVFTLSAHV FNSGNEVYID
RIEFVPAEVT FEAEYDLERA QKAVNELFTS SNQIGLKTDV TDYHIDQVSN LVECLSDEFC
LDEKKELSEK VKHAKRLSDE RNLLQDPNFR GINRQLDRGW RGSTDITIQG GDDVFKENYV
TLLGTFDECY PTYLYQKIDE SKLKAYTRYQ LRGYIEDSQD LEIYLIRYNA KHETVNVPGT
GSLWPLSAPS PIGKCAHHSH HFSLDIDVGC TDLNEDLGVW VIFKIKTQDG HARLGNLEFL
EEKPLVGEAL ARVKRAEKKW RDKREKLEWE TNIVYKEAKE SVDALFVNSQ YDRLQADTNI
AMIHAADKRV HSIREAYLPE LSVIPGVNAA IFEELEGRIF TAFSLYDARN VIKNGDFNNG
LSCWNVKGHV DVEEQNNHRS VLVVPEWEAE VSQEVRVCPG RGYILRVTAY KEGYGEGCVT
IHEIENNTDE LKFSNCVEEE VYPNNTVTCN DYTATQEEYE GTYTSRNRGY DGAYESNSSV
PADYASAYEE KAYTDGRRDN PCESNRGYGD YTPLPAGYVT KELEYFPETD KVWIEIGETE
GTFIVDSVEL LLMEE
