CR1AB_BACTK
ID CR1AB_BACTK Reviewed; 1155 AA.
AC P0A370; P06577; P06578; P09663; P09666; P09667; P21257; Q45789;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1988, sequence version 1.
DT 25-MAY-2022, entry version 64.
DE RecName: Full=Pesticidal crystal protein Cry1Ab;
DE AltName: Full=130 kDa crystal protein;
DE AltName: Full=Crystaline entomocidal protoxin;
DE AltName: Full=Insecticidal delta-endotoxin CryIA(b);
GN Name=cry1Ab; Synonyms=bt2, cry-1-2, cry1A(b), cryIA(b), cryIC1;
OS Bacillus thuringiensis subsp. kurstaki.
OG Plasmid pUCBtS93.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=29339;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=HD-1;
RX PubMed=3557124; DOI=10.1016/0378-1119(86)90357-4;
RA Geiser M., Schweitzer S., Grimm C.;
RT "The hypervariable region in the genes coding for entomopathogenic crystal
RT proteins of Bacillus thuringiensis: nucleotide sequence of the kurhd1 gene
RT of subsp. kurstaki HD1.";
RL Gene 48:109-118(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=HD-1;
RX PubMed=3011746; DOI=10.1128/jb.166.3.801-811.1986;
RA Thorne L., Garduno F., Thompson T., Decker D., Zounes M., Wild M.,
RA Walfield A.M., Pollock T.J.;
RT "Structural similarity between the lepidoptera- and diptera-specific
RT insecticidal endotoxin genes of Bacillus thuringiensis subsp. 'kurstaki'
RT and 'israelensis'.";
RL J. Bacteriol. 166:801-811(1986).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=HD-1;
RX AGRICOLA=IND87016057;
RA Kondo S., Tamura N., Kunitate A., Hattori M., Akashi A., Ohmori I.;
RT "Cloning and nucleotide sequencing of two insecticidal delta-endotoxin
RT genes from Bacillus thuringiensis var. kurstaki HD-1 DNA.";
RL Agric. Biol. Chem. 51:455-463(1987).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=HD-1;
RX DOI=10.1038/nbt0887-807;
RA Fischhoff D.A., Bowdish K.S., Perlak F.J., Marrone P.G., McCormick S.M.,
RA Niedermeyer J.G., Dean D.A., Kusano-Kretzmer K., Mayer E.J.,
RA Rochester D.E., Rogers S.G., Fraley R.T.;
RT "Insect tolerant transgenic tomato plants.";
RL Biotechnology (N.Y.) 5:807-813(1987).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=NRD-12;
RX DOI=10.1016/0168-1656(87)90061-7;
RA Hefford M.A., Brousseau R., Prefontaine G., Hanna Z., Condie J.A.,
RA Lau P.C.K.;
RT "Sequence of a lepidopteran toxin gene of Bacillus thuringiensis subsp
RT kurstaki NRD-12.";
RL J. Biotechnol. 6:307-322(1987).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S93; PLASMID=pUCBtS93;
RX DOI=10.1139/w99-032;
RA Silva-Werneck J.O., De-Souza M.T., Dias J.M.C.S., Ribeiro B.M.;
RT "Characterization of Bacillus thuringiensis subsp. kurstaki strain S93
RT effective against the Fall armyworm, Spodoptera frugiperda and cloning of a
RT cry1Ab gene.";
RL Can. J. Microbiol. 45:464-471(1999).
CC -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC epithelial cells of many lepidopteran larvae.
CC -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC and is accumulated both as an inclusion and as part of the spore coat.
CC -!- BIOTECHNOLOGY: Introduced by genetic manipulation and expressed in
CC insect-resistant maize by Monsanto, Northrup King and Ciba Geigy.
CC -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC terminus.
CC -!- MISCELLANEOUS: In strain S93 this is encoded on plasmid pUCBtS39.
CC {ECO:0000269|Ref.6}.
CC -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR EMBL; M15271; AAA22561.1; -; Genomic_DNA.
DR EMBL; M12661; AAA22613.1; -; Genomic_DNA.
DR EMBL; D00117; BAA00071.1; -; Genomic_DNA.
DR EMBL; M37263; AAA22420.1; -; Genomic_DNA.
DR EMBL; AF059670; AAC64003.1; -; Genomic_DNA.
DR EMBL; A03793; CAA00303.1; -; Unassigned_DNA.
DR EMBL; A09398; CAA00840.1; -; Unassigned_DNA.
DR PIR; A29125; A29125.
DR PIR; A29838; A29838.
DR PIR; A90025; JD0002.
DR PIR; I39838; I39838.
DR RefSeq; WP_000369819.1; NZ_PGEH01000375.1.
DR AlphaFoldDB; P0A370; -.
DR SMR; P0A370; -.
DR TCDB; 1.C.2.1.3; the channel-forming Delta-endotoxin insecticidal crystal protein (icp) family.
DR GeneID; 67470639; -.
DR GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 1.20.190.10; -; 1.
DR Gene3D; 2.100.10.10; -; 1.
DR InterPro; IPR041587; Cry_V.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR038979; Pest_crys.
DR InterPro; IPR005638; Pest_crys_C.
DR InterPro; IPR005639; Pest_crys_N.
DR InterPro; IPR036716; Pest_crys_N_sf.
DR InterPro; IPR001178; Pest_cryst_cen_dom.
DR InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR PANTHER; PTHR37003; PTHR37003; 1.
DR Pfam; PF17997; Cry1Ac_D5; 1.
DR Pfam; PF03944; Endotoxin_C; 1.
DR Pfam; PF00555; Endotoxin_M; 1.
DR Pfam; PF03945; Endotoxin_N; 1.
DR SUPFAM; SSF49785; SSF49785; 1.
DR SUPFAM; SSF51096; SSF51096; 1.
DR SUPFAM; SSF56849; SSF56849; 1.
PE 1: Evidence at protein level;
KW Genetically modified food; Plasmid; Sporulation; Toxin; Virulence.
FT CHAIN 1..1155
FT /note="Pesticidal crystal protein Cry1Ab"
FT /id="PRO_0000174023"
FT CONFLICT 207
FT /note="A -> R (in Ref. 2; AAA22613)"
FT /evidence="ECO:0000305"
FT CONFLICT 382..406
FT /note="VLDGTEFAYGTSSNLPSAVYRKSGT -> GPDGGRICLWNLLKFGQPPYTEK
FT AEP (in Ref. 2; AAA22613)"
FT /evidence="ECO:0000305"
FT CONFLICT 410
FT /note="L -> P (in Ref. 2; AAA22613)"
FT /evidence="ECO:0000305"
FT CONFLICT 430..432
FT /note="LSH -> CLAY (in Ref. 2; AAA22613)"
FT /evidence="ECO:0000305"
FT CONFLICT 437
FT /note="R -> Y (in Ref. 2; AAA22613)"
FT /evidence="ECO:0000305"
FT CONFLICT 447
FT /note="I -> V (in Ref. 2; AAA22613)"
FT /evidence="ECO:0000305"
FT CONFLICT 452..458
FT /note="MFSWIHR -> NDSSWTYC (in Ref. 2; AAA22613)"
FT /evidence="ECO:0000305"
FT CONFLICT 461
FT /note="E -> N (in Ref. 5; AAA22420)"
FT /evidence="ECO:0000305"
FT CONFLICT 463..465
FT /note="NNI -> GDV (in Ref. 2; AAA22613)"
FT /evidence="ECO:0000305"
FT CONFLICT 479..486
FT /note="STNLGSGT -> LQSWLWN (in Ref. 2; AAA22613)"
FT /evidence="ECO:0000305"
FT CONFLICT 492
FT /note="P -> L (in Ref. 2; AAA22613)"
FT /evidence="ECO:0000305"
FT CONFLICT 501..505
FT /note="RRTSP -> EELT (in Ref. 2; AAA22613)"
FT /evidence="ECO:0000305"
FT CONFLICT 542
FT /note="D -> H (in Ref. 5; AAA22420)"
FT /evidence="ECO:0000305"
FT CONFLICT 568..569
FT /note="TV -> HL (in Ref. 5; AAA22420)"
FT /evidence="ECO:0000305"
FT CONFLICT 665
FT /note="K -> E (in Ref. 5; AAA22420)"
FT /evidence="ECO:0000305"
FT CONFLICT 675..676
FT /note="KR -> NG (in Ref. 5; AAA22420)"
FT /evidence="ECO:0000305"
FT CONFLICT 703
FT /note="S -> N (in Ref. 2; AAA22613)"
FT /evidence="ECO:0000305"
FT CONFLICT 712
FT /note="D -> H (in Ref. 2; AAA22613)"
FT /evidence="ECO:0000305"
FT CONFLICT 832
FT /note="A -> E (in Ref. 4; no nucleotide entry)"
FT /evidence="ECO:0000305"
FT CONFLICT 836
FT /note="N -> I (in Ref. 5; AAA22420)"
FT /evidence="ECO:0000305"
FT CONFLICT 842..843
FT /note="EK -> GRA (in Ref. 4; no nucleotide entry)"
FT /evidence="ECO:0000305"
FT CONFLICT 1016
FT /note="E -> T (in Ref. 5; AAA22420)"
FT /evidence="ECO:0000305"
FT CONFLICT 1060
FT /note="E -> G (in Ref. 5; AAA22420)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1155 AA; 130624 MW; 43461A64C7AC7CAF CRC64;
MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF VPGAGFVLGL
VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL EGLSNLYQIY AESFREWEAD
PTNPALREEM RIQFNDMNSA LTTAIPLFAV QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ
RWGFDAATIN SRYNDLTRLI GNYTDHAVRW YNTGLERVWG PDSRDWIRYN QFRRELTLTV
LDIVSLFPNY DSRTYPIRTV SQLTREIYTN PVLENFDGSF RGSAQGIEGS IRSPHLMDIL
NSITIYTDAH RGEYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI VAQLGQGVYR
TLSSTLYRRP FNIGINNQQL SVLDGTEFAY GTSSNLPSAV YRKSGTVDSL DEIPPQNNNV
PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA PMFSWIHRSA EFNNIIPSSQ ITQIPLTKST
NLGSGTSVVK GPGFTGGDIL RRTSPGQIST LRVNITAPLS QRYRVRIRYA STTNLQFHTS
IDGRPINQGN FSATMSSGSN LQSGSFRTVG FTTPFNFSNG SSVFTLSAHV FNSGNEVYID
RIEFVPAEVT FEAEYDLERA QKAVNELFTS SNQIGLKTDV TDYHIDQVSN LVECLSDEFC
LDEKKELSEK VKHAKRLSDE RNLLQDPNFR GINRQLDRGW RGSTDITIQG GDDVFKENYV
TLLGTFDECY PTYLYQKIDE SKLKAYTRYQ LRGYIEDSQD LEIYLIRYNA KHETVNVPGT
GSLWPLSAPS PIGKCAHHSH HFSLDIDVGC TDLNEDLGVW VIFKIKTQDG HARLGNLEFL
EEKPLVGEAL ARVKRAEKKW RDKREKLEWE TNIVYKEAKE SVDALFVNSQ YDRLQADTNI
AMIHAADKRV HSIREAYLPE LSVIPGVNAA IFEELEGRIF TAFSLYDARN VIKNGDFNNG
LSCWNVKGHV DVEEQNNHRS VLVVPEWEAE VSQEVRVCPG RGYILRVTAY KEGYGEGCVT
IHEIENNTDE LKFSNCVEEE VYPNNTVTCN DYTATQEEYE GTYTSRNRGY DGAYESNSSV
PADYASAYEE KAYTDGRRDN PCESNRGYGD YTPLPAGYVT KELEYFPETD KVWIEIGETE
GTFIVDSVEL LLMEE
