CR1AC_BACTK
ID CR1AC_BACTK Reviewed; 1178 AA.
AC P05068;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 13-AUG-1987, sequence version 1.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=Pesticidal crystal protein Cry1Ac;
DE AltName: Full=133 kDa crystal protein;
DE AltName: Full=Crystaline entomocidal protoxin;
DE AltName: Full=Insecticidal delta-endotoxin CryIA(c);
GN Name=cry1Ac; Synonyms=cry1A(c), cry218, cryIA(c);
OS Bacillus thuringiensis subsp. kurstaki.
OG Plasmid 75 Kb.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=29339;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 35866 / NRRL B-4488 / HD-73;
RX PubMed=3000881; DOI=10.1016/0378-1119(85)90184-2;
RA Adang M.J., Staver M.J., Rocheleau T.A., Leighton J., Barker R.F.,
RA Thompson D.V.;
RT "Characterized full-length and truncated plasmid clones of the crystal
RT protein of Bacillus thuringiensis subsp. kurstaki HD-73 and their toxicity
RT to Manduca sexta.";
RL Gene 36:289-300(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 35866 / NRRL B-4488 / HD-73;
RX PubMed=9003589; DOI=10.1007/s002849900154;
RA Omolo E.O., James M.D., Osir E.O., Thomson J.A.;
RT "Cloning and expression of a Bacillus thuringiensis (L1-2) gene encoding a
RT crystal protein active against Glossina morsitans morsitans and Chilo
RT partellus.";
RL Curr. Microbiol. 34:118-121(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 35866 / NRRL B-4488 / HD-73;
RA Herrera G., James M.D.;
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DSIR732;
RA Gleave A.P., Hedges R.J., Broadwell A.H., Wigley P.J.;
RT "Cloning and nucleotide sequence of an insecticidal crystal protein gene
RT from Bacillus thuringiensis DSIR732 active against 3 species of leafroller
RT (Lepidoptera, Tortricidae).";
RL N. Z. J. Crop Hortic. Sci. 20:27-36(1992).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=YBT-1520;
RA Sun M., Yu Z.;
RL Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC epithelial cells of many lepidopteran larvae.
CC -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC and is accumulated both as an inclusion and as part of the spore coat.
CC -!- BIOTECHNOLOGY: Introduced by genetic manipulation and expressed in
CC insect-resistant cotton and tomato by Calgene (Monsanto) and in maize
CC by Dekalb Genetics.
CC -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC terminus.
CC -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR EMBL; M11068; AAA22331.1; -; Genomic_DNA.
DR EMBL; U87397; AAC44841.1; -; Genomic_DNA.
DR EMBL; U87793; AAB46989.1; -; Genomic_DNA.
DR EMBL; U89872; AAB49768.1; -; Genomic_DNA.
DR EMBL; AJ002514; CAA05505.1; -; Genomic_DNA.
DR PIR; A23962; USBSXH.
DR RefSeq; WP_000369821.1; NZ_PHSM01000059.1.
DR PDB; 4ARX; X-ray; 2.35 A; A/B/C/D=31-609.
DR PDB; 4ARY; X-ray; 2.95 A; A/B/C/D=31-611.
DR PDB; 4W8J; X-ray; 2.78 A; A=1-1178.
DR PDBsum; 4ARX; -.
DR PDBsum; 4ARY; -.
DR PDBsum; 4W8J; -.
DR AlphaFoldDB; P05068; -.
DR SMR; P05068; -.
DR ABCD; P05068; 7 sequenced antibodies.
DR GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 1.20.190.10; -; 1.
DR Gene3D; 2.100.10.10; -; 1.
DR InterPro; IPR041587; Cry_V.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR005638; Pest_crys_C.
DR InterPro; IPR005639; Pest_crys_N.
DR InterPro; IPR036716; Pest_crys_N_sf.
DR InterPro; IPR001178; Pest_cryst_cen_dom.
DR InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR Pfam; PF17997; Cry1Ac_D5; 1.
DR Pfam; PF03944; Endotoxin_C; 1.
DR Pfam; PF00555; Endotoxin_M; 1.
DR Pfam; PF03945; Endotoxin_N; 1.
DR SUPFAM; SSF49785; SSF49785; 1.
DR SUPFAM; SSF51096; SSF51096; 1.
DR SUPFAM; SSF56849; SSF56849; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Genetically modified food; Plasmid; Sporulation; Toxin;
KW Virulence.
FT CHAIN 1..1178
FT /note="Pesticidal crystal protein Cry1Ac"
FT /id="PRO_0000174024"
FT HELIX 35..48
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 51..53
FT /evidence="ECO:0007829|PDB:4ARX"
FT HELIX 54..63
FT /evidence="ECO:0007829|PDB:4ARX"
FT TURN 64..67
FT /evidence="ECO:0007829|PDB:4W8J"
FT HELIX 71..84
FT /evidence="ECO:0007829|PDB:4ARX"
FT HELIX 90..119
FT /evidence="ECO:0007829|PDB:4ARX"
FT HELIX 124..144
FT /evidence="ECO:0007829|PDB:4ARX"
FT HELIX 145..148
FT /evidence="ECO:0007829|PDB:4ARX"
FT TURN 154..157
FT /evidence="ECO:0007829|PDB:4ARX"
FT HELIX 158..182
FT /evidence="ECO:0007829|PDB:4ARX"
FT HELIX 186..217
FT /evidence="ECO:0007829|PDB:4ARX"
FT HELIX 223..239
FT /evidence="ECO:0007829|PDB:4ARX"
FT HELIX 241..244
FT /evidence="ECO:0007829|PDB:4ARX"
FT HELIX 245..250
FT /evidence="ECO:0007829|PDB:4ARX"
FT TURN 252..254
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 266..269
FT /evidence="ECO:0007829|PDB:4ARX"
FT HELIX 271..274
FT /evidence="ECO:0007829|PDB:4ARX"
FT HELIX 284..289
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 298..310
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 313..325
FT /evidence="ECO:0007829|PDB:4ARX"
FT HELIX 326..328
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 344..351
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 358..368
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 379..390
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 399..402
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 406..408
FT /evidence="ECO:0007829|PDB:4ARX"
FT HELIX 409..411
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 418..420
FT /evidence="ECO:0007829|PDB:4W8J"
FT HELIX 422..425
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 428..438
FT /evidence="ECO:0007829|PDB:4ARX"
FT TURN 441..443
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 445..457
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 468..475
FT /evidence="ECO:0007829|PDB:4ARX"
FT HELIX 476..478
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 480..488
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 492..496
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 498..501
FT /evidence="ECO:0007829|PDB:4ARX"
FT HELIX 508..510
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 513..517
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 527..534
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 539..545
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 548..555
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 562..564
FT /evidence="ECO:0007829|PDB:4ARX"
FT HELIX 567..569
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 571..574
FT /evidence="ECO:0007829|PDB:4ARX"
FT HELIX 581..583
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 585..591
FT /evidence="ECO:0007829|PDB:4ARX"
FT STRAND 598..607
FT /evidence="ECO:0007829|PDB:4ARX"
FT HELIX 610..626
FT /evidence="ECO:0007829|PDB:4W8J"
FT STRAND 629..631
FT /evidence="ECO:0007829|PDB:4W8J"
FT HELIX 643..654
FT /evidence="ECO:0007829|PDB:4W8J"
FT TURN 658..660
FT /evidence="ECO:0007829|PDB:4W8J"
FT HELIX 662..682
FT /evidence="ECO:0007829|PDB:4W8J"
FT STRAND 705..711
FT /evidence="ECO:0007829|PDB:4W8J"
FT STRAND 720..724
FT /evidence="ECO:0007829|PDB:4W8J"
FT STRAND 733..739
FT /evidence="ECO:0007829|PDB:4W8J"
FT HELIX 741..743
FT /evidence="ECO:0007829|PDB:4W8J"
FT STRAND 746..778
FT /evidence="ECO:0007829|PDB:4W8J"
FT STRAND 827..834
FT /evidence="ECO:0007829|PDB:4W8J"
FT TURN 840..842
FT /evidence="ECO:0007829|PDB:4W8J"
FT STRAND 846..852
FT /evidence="ECO:0007829|PDB:4W8J"
FT STRAND 854..856
FT /evidence="ECO:0007829|PDB:4W8J"
FT STRAND 858..871
FT /evidence="ECO:0007829|PDB:4W8J"
FT HELIX 874..910
FT /evidence="ECO:0007829|PDB:4W8J"
FT TURN 917..919
FT /evidence="ECO:0007829|PDB:4W8J"
FT HELIX 927..937
FT /evidence="ECO:0007829|PDB:4W8J"
FT HELIX 956..976
FT /evidence="ECO:0007829|PDB:4W8J"
FT TURN 985..990
FT /evidence="ECO:0007829|PDB:4W8J"
FT STRAND 998..1003
FT /evidence="ECO:0007829|PDB:4W8J"
FT STRAND 1006..1011
FT /evidence="ECO:0007829|PDB:4W8J"
FT STRAND 1018..1023
FT /evidence="ECO:0007829|PDB:4W8J"
FT STRAND 1030..1049
FT /evidence="ECO:0007829|PDB:4W8J"
FT STRAND 1051..1053
FT /evidence="ECO:0007829|PDB:4W8J"
FT STRAND 1056..1061
FT /evidence="ECO:0007829|PDB:4W8J"
FT STRAND 1142..1148
FT /evidence="ECO:0007829|PDB:4W8J"
FT STRAND 1151..1175
FT /evidence="ECO:0007829|PDB:4W8J"
SQ SEQUENCE 1178 AA; 133331 MW; 9B34F2ED410E88BB CRC64;
MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF VPGAGFVLGL
VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL EGLSNLYQIY AESFREWEAD
PTNPALREEM RIQFNDMNSA LTTAIPLFAV QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ
RWGFDAATIN SRYNDLTRLI GNYTDYAVRW YNTGLERVWG PDSRDWVRYN QFRRELTLTV
LDIVALFPNY DSRRYPIRTV SQLTREIYTN PVLENFDGSF RGSAQGIERS IRSPHLMDIL
NSITIYTDAH RGYYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI VAQLGQGVYR
TLSSTLYRRP FNIGINNQQL SVLDGTEFAY GTSSNLPSAV YRKSGTVDSL DEIPPQNNNV
PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA PMFSWIHRSA EFNNIIASDS ITQIPAVKGN
FLFNGSVISG PGFTGGDLVR LNSSGNNIQN RGYIEVPIHF PSTSTRYRVR VRYASVTPIH
LNVNWGNSSI FSNTVPATAT SLDNLQSSDF GYFESANAFT SSLGNIVGVR NFSGTAGVII
DRFEFIPVTA TLEAEYNLER AQKAVNALFT STNQLGLKTN VTDYHIDQVS NLVTYLSDEF
CLDEKRELSE KVKHAKRLSD ERNLLQDSNF KDINRQPERG WGGSTGITIQ GGDDVFKENY
VTLSGTFDEC YPTYLYQKID ESKLKAFTRY QLRGYIEDSQ DLEIYLIRYN AKHETVNVPG
TGSLWPLSAQ SPIGKCGEPN RCAPHLEWNP DLDCSCRDGE KCAHHSHHFS LDIDVGCTDL
NEDLGVWVIF KIKTQDGHAR LGNLEFLEEK PLVGEALARV KRAEKKWRDK REKLEWETNI
VYKEAKESVD ALFVNSQYDQ LQADTNIAMI HAADKRVHSI REAYLPELSV IPGVNAAIFE
ELEGRIFTAF SLYDARNVIK NGDFNNGLSC WNVKGHVDVE EQNNQRSVLV VPEWEAEVSQ
EVRVCPGRGY ILRVTAYKEG YGEGCVTIHE IENNTDELKF SNCVEEEIYP NNTVTCNDYT
VNQEEYGGAY TSRNRGYNEA PSVPADYASV YEEKSYTDGR RENPCEFNRG YRDYTPLPVG
YVTKELEYFP ETDKVWIEIG ETEGTFIVDS VELLLMEE
