ID   CR1AD_BACTA             Reviewed;        1179 AA.
AC   Q03744;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Pesticidal crystal protein Cry1Ad;
DE   AltName: Full=133 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryIA(d);
GN   Name=cry1Ad; Synonyms=cryIA(d);
OS   Bacillus thuringiensis subsp. aizawai.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1433;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NRRL B-18484 / PS81I;
RA   Payne J.M., Sick A.J.;
RT   "Bacillus thuringiensis isolate active against lepidopteran pests, and
RT   genes encoding novel lepidopteran-active toxins.";
RL   Patent number US5246852, 21-SEP-1993.
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of many lepidopteran larvae.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; M73250; AAA22340.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q03744; -.
DR   SMR; Q03744; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR038979; Pest_crys.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   PANTHER; PTHR37003; PTHR37003; 1.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Sporulation; Toxin; Virulence.
FT   CHAIN           1..1179
FT                   /note="Pesticidal crystal protein Cry1Ad"
FT                   /id="PRO_0000174025"
SQ   SEQUENCE   1179 AA;  133377 MW;  8BB639FCDF862FAD CRC64;
     MEIMNNQNQC VPYNCLNDPT IEILEGERIE TGYTPIDISL SLTQFLLSEF VPGAGFVLGL
     IDLIWGFVGP SQWDAFLVQI EQLINQRIEE FARNQAISRL EGLSNLYQIY AEAFREWEAD
     PTNPALTEEM RIQFNDMNSA LTTAIPLFTV QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ
     RWGFDVATIN SRYNDLTRLI GTYTDYAVRW YNTGLERVWG PDSRDWVRYN QFRRELTLTV
     LDIVSLFPNY DSRTYPIRTV SQLTREIYTN PVLENFDGSF RGMAQRIEQN IRQPHLMDLL
     NSITIYTDVH RGFNYWSGHQ ITASPVGFAG PEFTFPRYGT MGNAAPPVLI STTGLGIFRT
     LSSPLYRRII LGSGPNNQNL FVLDGTEFSF ASLTADLPST IYRQRGTVDS LDVIPPQDNS
     VPARAGFSHR LSHVTMLSQA AGAVYTLRAP TFSWRHRSAE FSNLIPSSQI TQIPLTKSIN
     LGSGTSVVKG PGFTGGDILR ITSPGQISTL RVTITAPLSQ RYRVRIRYAS TTNLQFHTSI
     DGRPINQGNF SATMSSGGNL QSGSFRTAGF TTPFNFSNGS SIFTLSAHVF NSGNEVYIER
     IEFVPAEVTF EAEYDLERAQ EAVNALFTSS NQLGLKTNVT DYHIDQVSNL VECLSGEFCL
     DEKRELSEKV KHANRLSDER NLLQDPNFRG INRQPDRGWR GSTDITIQGG DDVFKENYVT
     LPGTFNECYP TYLYQKIDES KLKAYTRYQL RGYIEDSQHL EIYLIRYNTK HETVNVPGTG
     SLWPLSVENP IGKCGEPNRC APQLEWNPDL DCSCRDGEKC AHHSHHFSLD IDIGCTDLNE
     NLGVWVIFKI KMQDGHARLG NLEFLEEKPL VGESLARVKR AEKKWRDKRE KLQVETNIVY
     KEAKESVDAL FVNSQYDRLQ ADTDIAMIHA ADKRVHRIRE AYLPELSVIP GVNAGIFEEL
     EGRIFTAYSL YDARNVIKNG DFNNGLSCWN VKGHVDVEEQ NNHRSVLVVP EWEAEVSQEV
     RVCPGRGYIL RVTAYKEGYG EGCVTIHEIE DNTDELKFSN CVEEEVYPNN TVTCNDYTAN
     QEEYGGAYTS RNRGYGESYE SNSSIPAEYA PVYEEAYIDG RKENPCESNR GYGDYTPLPA
     GYVTKELEYF PETDKVWIEI GETEGTFIVD SVELLLMEE