ID   CR1AE_BACTL             Reviewed;        1181 AA.
AC   Q03748;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Pesticidal crystal protein Cry1Ae;
DE   AltName: Full=134 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryIA(e);
GN   Name=cry1Ae; Synonyms=cryIA(e), endI;
OS   Bacillus thuringiensis subsp. alesti.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1440;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1655719; DOI=10.1128/jb.173.20.6635-6638.1991;
RA   Lee C.S., Aronson A.I.;
RT   "Cloning and analysis of delta-endotoxin genes from Bacillus thuringiensis
RT   subsp. alesti.";
RL   J. Bacteriol. 173:6635-6638(1991).
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of many lepidopteran larvae.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; M65252; AAA22410.1; -; Genomic_DNA.
DR   PIR; A41052; A41052.
DR   AlphaFoldDB; Q03748; -.
DR   SMR; Q03748; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Sporulation; Toxin; Virulence.
FT   CHAIN           1..1181
FT                   /note="Pesticidal crystal protein Cry1Ae"
FT                   /id="PRO_0000174026"
SQ   SEQUENCE   1181 AA;  133738 MW;  FE86D2138C37D0FD CRC64;
     MDNNPKINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF VPGAGFVLGL
     IDLIWGFVGP SQWDAFLVQI EQLISQRIEE FARNQAISRL EGLSNLYQIY AEAFREWEAD
     PTNPALREEM RIQFNDMNSA LTTAIPLFTV QNYQVPLLSV YVQAVNLHLS VLRDVSVFGQ
     RWGLDVATIN SRYNDLTRLI GTYTDYAVRW YNTGLERVWG PDSRDWVRYN QFRRELTLTV
     LDIVSLFPNY DSRTYPIRTV SQLTREIYTN PVLENFDGSF RGSAQRIEQS IRSPHLMDIL
     NSITIYTDAH GGYYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI VAQLGQGVYR
     TLSSTFYRNP FIIGINNQRL SVLDGTEFAY GSSSNLPSAV YRKSGTVDSL DEIPPQDNNV
     PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA PMFSWIHRSA EFNNIIPSSQ ITQIPLTKST
     NLGSGTSVVK GPGFTGGDIL RRTSPGQIST LRVNITAPLS QRYRVRIRYA STTNLQFHTS
     IDGRPINQGN FSATMSSGGN LQSGSFRTVG FTTPFNFSNG SSVFTLSAHV FNSGNEVYID
     RIEFVPAEVT FEAEYDLERA QEAVNALFTS PNQIGLKTDV TDYHIDQVSN LVECLSDEFC
     LDEKRELSEK VKHAKRLSDE RNLLQDPNFR GINRQPDRGW RGSTDITIQG GDDVFKENYV
     TLPGTFDECY PTYLYQKIDE SKLKAYTRYE LRGYIEDSQD LEIYLIRYNA KHETVNVPGT
     GSLWPLSFES SIGKCGEPNR CAPHLEWNPD LDCSCRDGEK CAHHSHHFSL DIDVGCIDLN
     EDLGVWVIFK IKTQDGHARL GNLEFLEEKP LVGEALARVK RAEKKWRDKR EKLQLETNIV
     YKEAKESVDA LFVNSQYDQL QADTNIAMIH TADKRVHRIQ EAYLPELSVI PGVNAGIFEE
     LEGRIFTAYS LYDARNVIKN GDFNNGLSCW NVKGHVDVEE QNNHRSVLVV PEWEAEVSQE
     VRVCPGRGYI LRVTAYKEGY GEGCVTIHEI ENNTDELKFS NCVEEEVYPN NTVTCNEYTA
     NQEEYGGAYT SCNRGYDETY GSNYSVPADY ASVYEEKAYT DGRRENPCES NRGYGDYTPL
     PAGYVTKQLE YFPETDKVWI EIGETEGTFI VDSVELFLME E