ID   CR1AF_BACTU             Reviewed;         911 AA.
AC   P96315;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Pesticidal crystal protein Cry1Af;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryIA(f);
DE   Flags: Fragment;
GN   Name=cry1Af; Synonyms=cryIA(f), icp;
OS   Bacillus thuringiensis.
OG   Plasmid pKSKNT.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1428;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NT0423;
RA   Kang S.K., Kim H.S., Yu Y.M.;
RT   "Partial crystal protein gene sequence of Bacillus thuringiensis NT0423
RT   isolated from soil in Korea.";
RL   Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of both dipteran and lepidopteran larvae.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; U82003; AAB82749.1; -; Genomic_DNA.
DR   AlphaFoldDB; P96315; -.
DR   SMR; P96315; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Plasmid; Sporulation; Toxin; Virulence.
FT   CHAIN           1..>911
FT                   /note="Pesticidal crystal protein Cry1Af"
FT                   /id="PRO_0000174027"
FT   NON_TER         911
SQ   SEQUENCE   911 AA;  103181 MW;  8201620E0D7AC864 CRC64;
     MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF VPGAGFVLGL
     VDIIWEIFSV LSSDAFLVQI EQLINQRIEE FARNQAISRL EGLSNLYQIY AESFREWEAD
     PTNPALREEM RIQFNDMNSA LTTAIPLFAV QNYQVPLLSV YVTCNYIISS ESVMCGQRSG
     FDAATINSRY NDLTRLIGNY TDHAVRWYNT GLERVWGPDS RDWIRYNQFR RELTLTVLDI
     VSLFPNYDSR TYPIRTVSQL TREIYTNPVL ENFDGSFRGS AQGIEGSIRS PHLMDILNSI
     TIYTDAHRGE YYWSGHQIMA SPVGFSGPEF TFPLYGTMGN SAPQQRIVAQ LGQGVYRTLS
     STLYRRPFNI GINNQQLSVL DGTEFAYGTS SNLPSAVYRK SGTVDSLDEI PPQNNNVPPR
     QGFSHRLSHV SMFRSGFSNS SVSIIRAPMF SWIHRSAEFN NIIPSSQITQ IPLTKSTNLG
     SGTSVVKGPG FTGGDILRRT SPGQISTLRV NITAPLSQRY RVRILYASTT NLQFHTSIDG
     RPINQGNFSA TMSSGRNLQS GSLRTVGFTT PFNFSNGSSV FTLSAHVFNS GNEVYIDRIE
     FVPAEVTFEA EYDLERAQKA VNELFTSSNQ IGLKTDVTDY HIDQVSNLVE CLSDEFCLDE
     KKELSEKVKH AKRLSDERNL LQDPNFRGIN RQLDRGWRRS TDITTQGGDD FFQENYVTLL
     GTLMGFPSIY IKKWWFEIKS LYPLPIRGYI EDSQDLEIYL IRYNAKHETV NVPGTVPYGR
     FQPQVPIGKC AHHSHHFSLD IDVGCTDLNE DLGVWVIFKI KTQDGHARLG NLEFLEEKPL
     VGEALARVKR AEKKWRDKRE KLEWETNIVY KEAKESVDAL FVNSQYDRLQ ADTNIAMIHA
     ADKRVHSIRE A