ID   CR1AG_BACTU             Reviewed;        1176 AA.
AC   Q9S515;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Pesticidal crystal protein Cry1Ag;
DE   AltName: Full=134 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryIA(g);
GN   Name=cry1Ag; Synonyms=cry1, cryIA(g);
OS   Bacillus thuringiensis.
OG   Plasmid.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1428;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Mustafa S.A.;
RT   "Cloning, nucleotide sequence and expression of a new gene encoding
RT   lepidopteran-specific toxin.";
RL   Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of many lepidopteran larvae.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- MISCELLANEOUS: Encoded on an unnamed plasmid. {ECO:0000305|Ref.1}.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; AF081248; AAD46137.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9S515; -.
DR   SMR; Q9S515; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR038979; Pest_crys.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   PANTHER; PTHR37003; PTHR37003; 1.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Plasmid; Sporulation; Toxin; Virulence.
FT   CHAIN           1..1176
FT                   /note="Pesticidal crystal protein Cry1Ag"
FT                   /id="PRO_0000174028"
SQ   SEQUENCE   1176 AA;  133394 MW;  2B5929160A2612BB CRC64;
     MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF VPGAGFVLGL
     VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL EGLSNLYQIY AESFREWEAD
     PTNPALREEM RIQFNDMNSA LTTAIPLLAV QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ
     RWGFDAATIN SRYNDLTRLI GNYTDYAVRW YNTGLERVWG PDSRDWVRYN QFRRELTLTV
     LDIVALFSNY DSRRYPIRTV SQLTREIYTN PVLENFDGSF RGMAQRIEPE YRQPHLMDIL
     NSISIYTDVH RGFNYWSGHQ ITTSPVGFSG PEFTFPLYGT YGNAAPPQRI AQTGLGIFRT
     LSSPLYRRII LGSGPNNQEL FVLDGTEFSF ASLTTNLPST IYRQRGTVDS LDVIPPQDNS
     VPPRAGFSHR LSHVPMLSQA AGAVYTLRAS LFLLLVLLIH ARSIFNNIIP SSQITQSFKK
     IISWTSVVKG PGFTGGDILR RPSPGLISTL RVNITAPLSQ RYRVRIRYAF TTNLQFLTSI
     DGRPINQGNF YATMSSGSNL QSGSFRTVGF TTPFNFSNGS SVFTLSAHVF NSGNEVYIDR
     IEFVPAEVTF EAEYDLERAQ NGVNQLFTSS NQIGLKTDGT DYHIDQVSNL VECLSDEFCL
     DEKQELSEKV KHAKRLSDER NLLQDPNFRG INRQLDRGWR GSHDITIQGG DDVFKENYVT
     LLGTFDECYP TYLYQKIDQS KLKAYTSYQL RGYIEDSQDL EIYLIGYNAK QQTVNVPGTG
     SLWPVYAPKP IGKCGEPNRC APHLEWNPDL DCSCRDGEKC AHHSLHFSID IDVGCTDLNE
     DLGVWVIFKI KTEDGHARLG NLEFLEEKPL VGEALARVKR AEKKWRDKRV KLEWETNIVY
     KEAKESVDAL FVNSQYDQLQ ADTNIAMIHA ADKRVHSIRE AYLPELSVIP GVNAAIFEEL
     EGRIFTAFSL YDARNVIKNG DFNNGLSCWN VKGHVDVEEQ NNQRSVLVVP EWEAEVSQEV
     RVCPGRGYFP RVTAYKEGYG EGCVTIHEIE NNTDELKFSN CVEEEIYPNN TVTCNDYTVN
     QEEYGGAYTS RNRGYNEAPS VPADYASVYE EKSYTDGRRE NPCEFNRGYR DYTGLPVGYV
     TKALEYFPET DKVWIEIGET EGTFIVDSVE LLLMEE