ID   CR1BE_BACTU             Reviewed;        1227 AA.
AC   O85805;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Pesticidal crystal protein Cry1Be;
DE   AltName: Full=139 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryIB(e);
GN   Name=cry1Be; Synonyms=158C2B, cryIB(e);
OS   Bacillus thuringiensis.
OG   Plasmid pMYC2383.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1428;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NRRL B-18872 / PS158C2;
RA   Payne J.M., Cummings D.A., Cannon R.J.C., Narva K.E., Stelman S.;
RT   "Bacillus thuringiensis genes encoding lepidopteran-active toxins.";
RL   Patent number US5723758, 03-MAR-1998.
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of many lepidopteran larvae.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; AF077326; AAC32850.1; -; Genomic_DNA.
DR   PDB; 6OWK; X-ray; 2.70 A; A=35-510.
DR   PDBsum; 6OWK; -.
DR   AlphaFoldDB; O85805; -.
DR   SMR; O85805; -.
DR   PRIDE; O85805; -.
DR   ABCD; O85805; 3 sequenced antibodies.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR038979; Pest_crys.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   PANTHER; PTHR37003; PTHR37003; 1.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Plasmid; Sporulation; Toxin; Virulence.
FT   CHAIN           1..1227
FT                   /note="Pesticidal crystal protein Cry1Be"
FT                   /id="PRO_0000174034"
FT   HELIX           55..66
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   HELIX           67..69
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   HELIX           77..89
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   HELIX           96..108
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   HELIX           114..141
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   HELIX           150..171
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   TURN            178..181
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   HELIX           182..206
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   HELIX           210..241
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   HELIX           247..263
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   HELIX           265..268
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   HELIX           269..274
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   TURN            276..278
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   STRAND          281..284
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   STRAND          290..292
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   TURN            309..311
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   STRAND          312..314
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   HELIX           318..325
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   STRAND          334..344
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   STRAND          347..364
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   STRAND          371..376
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   STRAND          385..388
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   STRAND          393..405
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   STRAND          410..422
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   TURN            424..426
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   STRAND          429..435
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   STRAND          438..447
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   HELIX           448..451
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   HELIX           461..464
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   STRAND          467..477
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   STRAND          480..489
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   STRAND          500..507
FT                   /evidence="ECO:0007829|PDB:6OWK"
FT   HELIX           508..510
FT                   /evidence="ECO:0007829|PDB:6OWK"
SQ   SEQUENCE   1227 AA;  139085 MW;  CBA847BEA0B34CD3 CRC64;
     MTSNRKNENE IINALSIPAV SNHSAQMNLS TDARIEDSLC IAEGNNIDPF VSASTVQTGI
     NIAGRILGVL GVPFAGQIAS FYSFLVGELW PRGRDPWEIF LEHVEQLIRQ QVTENTRDTA
     LARLQGLGNS FRAYQQSLED WLENRDDART RSVLYTQYIA LELDFLNAMP LFAIRNQEVP
     LLMVYAQAAN LHLLLLRDAS LFGSEFGLTS QEIQRYYERQ VEKTREYSDY CARWYNTGLN
     NLRGTNAESW LRYNQFRRDL TLGVLDLVAL FPSYDTRVYP MNTSAQLTRE IYTDPIGRTN
     APSGFASTNW FNNNAPSFSA IEAAVIRPPH LLDFPEQLTI FSVLSRWSNT QYMNYWVGHR
     LESRTIRGSL STSTHGNTNT SINPVTLQFT SRDVYRTESF AGINILLTTP VNGVPWARFN
     WRNPLNSLRG SLLYTIGYTG VGTQLFDSET ELPPETTERP NYESYSHRLS NIRLISGNTL
     RAPVYSWTHR SADRTNTISS DSITQIPLVK SFNLNSGTSV VSGPGFTGGD IIRTNVNGSV
     LSMGLNFNNT SLQRYRVRVR YAASQTMVLR VTVGGSTTFD QGFPSTMSAN ESLTSQSFRF
     AEFPVGISAS GSQTAGISIS NNAGRQTFHF DKIEFIPITA TFEAEYDLER AQEAVNALFT
     NTNPRRLKTG VTDYHIDEVS NLVACLSDEF CLDEKRELLE KVKYAKRLSD ERNLLQDPNF
     TSINKQPDFI STNEQSNFTS IHEQSEHGWW GSENITIQEG NDVFKENYVI LPGTFNECYP
     TYLYQKIGEA ELKAYTRYQL SGYIEDSQDL EIYLIRYNAK HETLDVPGTE SVWPLSVESP
     IGRCGEPNRC APHFEWNPDL DCSCRDGEKC AHHSHHFSLD IDVGCIDLHE NLGVWVVFKI
     KTQEGHARLG NLEFIEEKPL LGEALSRVKR AEKKWRDKRE KLQLETKRVY TEAKEAVDAL
     FVDSQYDRLQ ADTNIGMIHA ADKLVHRIRE AYLSELSVIP GVNAEIFEEL EGRIITAISL
     YDARNVVKNG DFNNGLACWN VKGHVDVQQS HHRSVLVIPE WEAEVSQAVR VCPGRGYILR
     VTAYKEGYGE GCVTIHEIEN NTDELKFKNC EEEEVYPTDT GTCNDYTAHQ GTAACNSRNA
     GYEDAYEVDT TASVNYKPTY EEETYTDVRR DNHCEYDRGY VNYPPVPAGY MTKELEYFPE
     TDKVWIEIGE TEGKFIVDSV ELLLMEE