CR1DA_BACTA
ID CR1DA_BACTA Reviewed; 1165 AA.
AC P19415;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1990, sequence version 1.
DT 25-MAY-2022, entry version 89.
DE RecName: Full=Pesticidal crystal protein Cry1Da;
DE AltName: Full=132 kDa crystal protein;
DE AltName: Full=Crystaline entomocidal protoxin;
DE AltName: Full=Insecticidal delta-endotoxin CryID(a);
GN Name=cry1Da; Synonyms=bt4, cryID, cryID(a);
OS Bacillus thuringiensis subsp. aizawai.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=1433;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=HD-68;
RX PubMed=2216728; DOI=10.1093/nar/18.18.5545;
RA Hoefte H., Soetaert P., Jansens S., Peferoen M.;
RT "Nucleotide sequence and deduced amino acid sequence of new Lepidoptera-
RT specific crystal protein gene from Bacillus thuringiensis.";
RL Nucleic Acids Res. 18:5545-5545(1990).
CC -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC epithelial cells of many lepidopteran larvae.
CC -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC and is accumulated both as an inclusion and as part of the spore coat.
CC -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC terminus.
CC -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR EMBL; X54160; CAA38099.1; -; Genomic_DNA.
DR PIR; S11446; S11446.
DR RefSeq; WP_042991627.1; NZ_NFEV01000141.1.
DR PDB; 6OVB; X-ray; 2.61 A; A=27-603.
DR PDBsum; 6OVB; -.
DR AlphaFoldDB; P19415; -.
DR SMR; P19415; -.
DR GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 1.20.190.10; -; 1.
DR Gene3D; 2.100.10.10; -; 1.
DR InterPro; IPR041587; Cry_V.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR038979; Pest_crys.
DR InterPro; IPR005638; Pest_crys_C.
DR InterPro; IPR005639; Pest_crys_N.
DR InterPro; IPR036716; Pest_crys_N_sf.
DR InterPro; IPR001178; Pest_cryst_cen_dom.
DR InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR PANTHER; PTHR37003; PTHR37003; 1.
DR Pfam; PF17997; Cry1Ac_D5; 1.
DR Pfam; PF03944; Endotoxin_C; 1.
DR Pfam; PF00555; Endotoxin_M; 1.
DR Pfam; PF03945; Endotoxin_N; 1.
DR SUPFAM; SSF49785; SSF49785; 2.
DR SUPFAM; SSF51096; SSF51096; 1.
DR SUPFAM; SSF56849; SSF56849; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Sporulation; Toxin; Virulence.
FT CHAIN 1..1165
FT /note="Pesticidal crystal protein Cry1Da"
FT /id="PRO_0000174038"
FT HELIX 33..47
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 50..52
FT /evidence="ECO:0007829|PDB:6OVB"
FT HELIX 53..62
FT /evidence="ECO:0007829|PDB:6OVB"
FT TURN 63..66
FT /evidence="ECO:0007829|PDB:6OVB"
FT HELIX 69..83
FT /evidence="ECO:0007829|PDB:6OVB"
FT HELIX 89..118
FT /evidence="ECO:0007829|PDB:6OVB"
FT HELIX 123..143
FT /evidence="ECO:0007829|PDB:6OVB"
FT HELIX 144..147
FT /evidence="ECO:0007829|PDB:6OVB"
FT TURN 153..156
FT /evidence="ECO:0007829|PDB:6OVB"
FT HELIX 157..181
FT /evidence="ECO:0007829|PDB:6OVB"
FT HELIX 185..216
FT /evidence="ECO:0007829|PDB:6OVB"
FT HELIX 222..238
FT /evidence="ECO:0007829|PDB:6OVB"
FT HELIX 240..243
FT /evidence="ECO:0007829|PDB:6OVB"
FT HELIX 244..249
FT /evidence="ECO:0007829|PDB:6OVB"
FT TURN 251..253
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 265..267
FT /evidence="ECO:0007829|PDB:6OVB"
FT HELIX 280..282
FT /evidence="ECO:0007829|PDB:6OVB"
FT HELIX 286..293
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 301..313
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 316..328
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 331..337
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 347..354
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 357..359
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 361..368
FT /evidence="ECO:0007829|PDB:6OVB"
FT HELIX 369..371
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 376..389
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 391..395
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 399..401
FT /evidence="ECO:0007829|PDB:6OVB"
FT HELIX 402..405
FT /evidence="ECO:0007829|PDB:6OVB"
FT HELIX 415..418
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 421..431
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 433..446
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 459..464
FT /evidence="ECO:0007829|PDB:6OVB"
FT HELIX 465..467
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 475..478
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 482..486
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 488..496
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 500..504
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 506..508
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 510..529
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 531..533
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 536..540
FT /evidence="ECO:0007829|PDB:6OVB"
FT HELIX 552..554
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 556..566
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 568..577
FT /evidence="ECO:0007829|PDB:6OVB"
FT STRAND 581..591
FT /evidence="ECO:0007829|PDB:6OVB"
SQ SEQUENCE 1165 AA; 132482 MW; BC265E5F187E08E7 CRC64;
MEINNQNQCV PYNCLSNPKE IILGEERLET GNTVADISLG LINFLYSNFV PGGGFIVGLL
ELIWGFIGPS QWDIFLAQIE QLISQRIEEF ARNQAISRLE GLSNLYKVYV RAFSDWEKDP
TNPALREEMR IQFNDMNSAL ITAIPLFRVQ NYEVALLSVY VQAANLHLSI LRDVSVFGER
WGYDTATINN RYSDLTSLIH VYTNHCVDTY NQGLRRLEGR FLSDWIVYNR FRRQLTISVL
DIVAFFPNYD IRTYPIQTAT QLTREVYLDL PFINENLSPA ASYPTFSAAE SAIIRSPHLV
DFLNSFTIYT DSLARYAYWG GHLVNSFRTG TTTNLIRSPL YGREGNTERP VTITASPSVP
IFRTLSYITG LDNSNPVAGI EGVEFQNTIS RSIYRKSGPI DSFSELPPQD ASVSPAIGYS
HRLCHATFLE RISGPRIAGT VFSWTHRSAS PTNEVSPSRI TQIPWVKAHT LASGASVIKG
PGFTGGDILT RNSMGELGTL RVTFTGRLPQ SYYIRFRYAS VANRSGTFRY SQPPSYGISF
PKTMDAGEPL TSRSFAHTTL FTPITFSRAQ EEFDLYIQSG VYIDRIEFIP VTATFEAEYD
LERAQKVVNA LFTSTNQLGL KTDVTDYHID QVSNLVACLS DEFCLDEKRE LSEKVKHAKR
LSDERNLLQD PNFRGINRQP DRGWRGSTDI TIQGGDDVFK ENYVTLPGTF DECYPTYLYQ
KIDESKLKAY TRYQLRGYIE DSQDLEIYLI RYNAKHEIVN VPGTGSLWPL SVENQIGPCG
EPNRCAPHLE WNPDLHCSCR DGEKCAHHSH HFSLDIDVGC TDLNEDLGVW VIFKIKTQDG
HARLGNLEFL EEKPLLGEAL ARVKRAEKKW RDKRETLQLE TTIVYKEAKE SVDALFVNSQ
YDRLQADTNI AMIHAADKRV HRIREAYLPE LSVIPGVNAA IFEELEERIF TAFSLYDARN
IIKNGDFNNG LLCWNVKGHV EVEEQNNHRS VLVIPEWEAE VSQEVRVCPG RGYILRVTAY
KEGYGEGCVT IHEIENNTDE LKFNNCVEEE VYPNNTVTCI NYTATQEEYE GTYTSRNRGY
DEAYGNNPSV PADYASVYEE KSYTDRRREN PCESNRGYGD YTPLPAGYVT KELEYFPETD
KVWIEIGETE GTFIVDSVEL LLMEE
