ID   CR1EA_BACTX             Reviewed;        1171 AA.
AC   Q57458; Q03741;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Pesticidal crystal protein Cry1Ea;
DE   AltName: Full=133 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryIE(a);
GN   Name=cry1Ea; Synonyms=bt11, btxI, cryIC(b), cryIE(a);
OS   Bacillus thuringiensis subsp. kenyae.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=33930;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2259636; DOI=10.1093/nar/18.24.7443;
RA   Bosse M., Masson L., Brousseau R.;
RT   "Nucleotide sequence of a novel crystal protein gene isolated from Bacillus
RT   thuringiensis subspecies kenyae.";
RL   Nucleic Acids Res. 18:7443-7443(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=4F1;
RX   PubMed=2254254; DOI=10.1128/jb.172.12.6783-6788.1990;
RA   Visser B., Munsterman E., Stoker A., Dirkse W.G.;
RT   "A novel Bacillus thuringiensis gene encoding a Spodoptera exigua-specific
RT   crystal protein.";
RL   J. Bacteriol. 172:6783-6788(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PS81F;
RA   Payne J.M., Sick A.J.;
RT   "Novel Bacillus thuringiensis isolate denoted B.t. PS81F, active against
RT   lepidopteran pests, and a gene encoding a lepidopteran-active toxin.";
RL   Patent number US5039523, 13-AUG-1991.
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of many lepidopteran larvae including Spodoptera
CC       species.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; X56144; CAA39609.1; -; Genomic_DNA.
DR   EMBL; X53985; CAA37933.1; -; Genomic_DNA.
DR   EMBL; M73252; AAA22345.1; -; Genomic_DNA.
DR   PIR; I40572; I40572.
DR   AlphaFoldDB; Q57458; -.
DR   SMR; Q57458; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR038979; Pest_crys.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   PANTHER; PTHR37003; PTHR37003; 1.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Sporulation; Toxin; Virulence.
FT   CHAIN           1..1171
FT                   /note="Pesticidal crystal protein Cry1Ea"
FT                   /id="PRO_0000174040"
FT   REGION          1094..1124
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1105..1120
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        862
FT                   /note="N -> K (in Ref. 3; AAA22345)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1171 AA;  133253 MW;  5758FBE5ABDB5ABE CRC64;
     MEIVNNQNQC VPYNCLNNPE NEILDIERSN STVATNIALE ISRLLASATP IGGILLGLFD
     AIWGSIGPSQ WDLFLEQIEL LIDQKIEEFA RNQAISRLEG ISSLYGIYTE AFREWEADPT
     NPALKEEMRT QFNDMNSILV TAIPLFSVQN YQVPFLSVYV QAANLHLSVL RDVSVFGQAW
     GFDIATINSR YNDLTRLIPI YTDYAVRWYN TGLDRLPRTG GLRNWARFNQ FRRELTISVL
     DIISFFRNYD SRLYPIPTSS QLTREVYTDP VINITDYRVG PSFENIENSA IRSPHLMDFL
     NNLTIDTDLI RGVHYWAGHR VTSHFTGSSQ VITTPQYGIT ANAEPRRTIA PSTFPGLNLF
     YRTLSNPFFR RSENITPTLG INVVQGVGFI QPNNAEVLYR SRGTVDSLNE LPIDGENSLV
     GYSHRLSHVT LTRSLYNTNI TSLPTFVWTH HSATNTNTIN PDIITQIPLV KGFRLGGGTS
     VIKGPGFTGG DILRRNTIGE FVSLQVNINS PITQRYRLRF RYASSRDARI TVAIGGQIRV
     DMTLEKTMEI GESLTSRTFS YTNFSNPFSF RANPDIIRIA EELPIRGGEL YIDKIELILA
     DATFEEEYDL ERAQKAVNAL FTSTNQLGLK TDVTDYHIDQ VSNLVECLSD EFCLDEKREL
     SEKVKHAKRL SDERNLLQDP NFRGINRQPD RGWRGSTDIT IQGGDDVFKE NYVTLPGTFD
     ECYPTYLYQK IDESKLKAYT RYELRGYIED SQDLEIYLIR YNAKHETVNV PGTGSLWPLS
     AQSPIGKCGE PNRCAPHLEW NPNLDCSCRD GEKCAHHSHH FSLDIDVGCT DLNEDLGVWV
     IFKIKTQDGY ARLGNLEFLE ENPLLGEALA RVKRAEKKWR DKCEKLEWET NIVYKEAKES
     VDALFVNSQY DRLQADTNIA MIHAADKRVH SIREAYLPEL SVIPGVNAAI FEELEGRIFT
     AFSLYDARNV IKNGDFNNGL SCWNVKGHVD VEEQNNHRSV LVVPEWEAEV SQEVRVCPGR
     GYILRVTAYK EGYGEGCVTI HEIEDNTDEL KFSNCVEEEV YPNNTVTCNN YTATQEEHEG
     TYTSRNRGYD EAYESNSSVH ASVYEEKSYT DRRRENPCES NRGYGDYTPL PAGYVTKELE
     YFPETDKVWI EIGETEGTFI VDSVELLLME E