ID   CR1EB_BACTA             Reviewed;        1174 AA.
AC   Q03745;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Pesticidal crystal protein Cry1Eb;
DE   AltName: Full=134 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryIE(b);
GN   Name=cry1Eb; Synonyms=cryIE(b);
OS   Bacillus thuringiensis subsp. aizawai.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1433;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NRRL B-18457 / PS81A2;
RA   Payne J.M., Sick A.J.;
RT   "Genes encoding lepidopteran-active toxins and transformed hosts.";
RL   Patent number US5206166, 27-APR-1993.
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of many lepidopteran larvae.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; M73253; AAA22346.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q03745; -.
DR   SMR; Q03745; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR038979; Pest_crys.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   PANTHER; PTHR37003; PTHR37003; 1.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Sporulation; Toxin; Virulence.
FT   CHAIN           1..1174
FT                   /note="Pesticidal crystal protein Cry1Eb"
FT                   /id="PRO_0000174041"
SQ   SEQUENCE   1174 AA;  133603 MW;  5EEA0BA7FD63480F CRC64;
     MENNIENQCI PYNCLNNPEV EILGIERSNS NVAAEIGLGL SRLLVSRIPL GDFILGLFDV
     IWGAIGPSQW DIFLEQIELL IGQRIEEFAR NQAISRLQGL SNLYRIYTNA FKNWEVDPTN
     PALREEMRIQ FNDMNSALTT AIPLFSVQGY EIPLLSVYVQ AANLHLSVLR DVSVFGQRWG
     FDVATINSRY NDLTRLIGEY TDYAVRWYNT GLNRLPRNEG VRGWARFNRF RRELTISVLD
     IISFFQNYDS RLYPIPTIYQ LTREVYTDPV INITDYRVTP SFESIENSAI RSPHLMDFLN
     NIIIDTDLIR GVHYWAGHRV TSHFTGSSQV ISSPQYGITA NAEPSRTIAP STFPGLNLFY
     RTLSDPFFRR SDNIMPTLGI NVVQGVGFIQ PNNGEVLYRR RGTVDSLDEL PIDGENSLVG
     YSHRLSHVTL TRSLYNTNIT SLPTFVWTHH SATDRNIIYP DVITQIPLVK SFSLTSGTSV
     VRGPGFTGGD IIRTNVNGNV LSMSLNFSNT SLQRYRVRVR YAASQTMVMR VNVGGSTTFD
     QGFPSTMSAN GSLTSQSFRF AEFPVGISTS GSQTAGISIS NNPGRQTFHL DRIEFIPVDA
     TFEAEYDLER AQKAVNSLFT SSNQIELKTD VTDYHIDQVS NLVDCLSDEF CLDEKRELSE
     KVKHAKRLSD ERNLLQDPNF RGINRQPDRG WRGSTDITIQ GGDDVFKENY VTLPGTFDEC
     YPTYLYQKID ESKLKAYNRY QLRGYIEDSQ DLEIYLIRYN AKHETVNVPG TGSLWPLSVE
     SPIGRCGEPN RCVPHLEWNP DLDCSCRDGE KCAHHSHHFS LDIDVGCTDL QEDLGVWVVF
     KIKTQEGYAR LGNLEFIEEK PLIGEALSRV KRAEKKWRDK REKLQLETKR VYTEAKEAVD
     ALFVDSQYDR LQADTNIGMI HAADRLVHQI HEAYLPELPF IPGINVVIFE ELENRISTAL
     SLYDARNVIK NGDFNNGLSC WNVKGHVDVV EQNNHRSVLV VPEWEAEVSQ TIRVCPGRGY
     ILRVTAYKEG YGEGCVTIHE IENNTDELKF KNCEEEEVYP TDTGTCNDYT AHQGTAGSTD
     SCNSRNIRYE DAYEMNTTAS VNYKPTYEEE RYTDVQGDNH CEYDRGYVNY RPVPAGYVTK
     ELEYFPETDK VWIEIGETEG KFIVDNVELL LMEE