ID   CR1FA_BACTA             Reviewed;        1174 AA.
AC   Q03746;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Pesticidal crystal protein Cry1Fa;
DE   AltName: Full=134 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryIF(a);
GN   Name=cry1Fa; Synonyms=cryIF, cryIF(a);
OS   Bacillus thuringiensis subsp. aizawai.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1433;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=EG6346;
RX   PubMed=2061280; DOI=10.1128/jb.173.13.3966-3976.1991;
RA   Chambers J.A., Jelen A., Gilbert M.P., Jany C.S., Johnson T.B.,
RA   Gawron-Burke C.;
RT   "Isolation and characterization of a novel insecticidal crystal protein
RT   gene from Bacillus thuringiensis subsp. aizawai.";
RL   J. Bacteriol. 173:3966-3976(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NRRL B-18484 / PS81I;
RA   Payne J.M., Sick A.J.;
RT   "Bacillus thuringiensis isolate active against lepidopteran pests, and
RT   genes encoding novel lepidopteran-active toxins.";
RL   Patent number US5188960, 23-FEB-1993.
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of many lepidopteran larvae.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; M63897; AAA22348.1; -; Genomic_DNA.
DR   EMBL; M73254; AAA22347.1; -; Genomic_DNA.
DR   PIR; A42459; A42459.
DR   PDB; 6DJ4; X-ray; 3.01 A; A=470-612.
DR   PDBsum; 6DJ4; -.
DR   AlphaFoldDB; Q03746; -.
DR   SMR; Q03746; -.
DR   PRIDE; Q03746; -.
DR   ABCD; Q03746; 4 sequenced antibodies.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR038979; Pest_crys.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   PANTHER; PTHR37003; PTHR37003; 1.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Sporulation; Toxin; Virulence.
FT   CHAIN           1..1174
FT                   /note="Pesticidal crystal protein Cry1Fa"
FT                   /id="PRO_0000174042"
FT   STRAND          473..475
FT                   /evidence="ECO:0007829|PDB:6DJ4"
FT   STRAND          479..482
FT                   /evidence="ECO:0007829|PDB:6DJ4"
FT   STRAND          486..490
FT                   /evidence="ECO:0007829|PDB:6DJ4"
FT   STRAND          492..508
FT                   /evidence="ECO:0007829|PDB:6DJ4"
FT   STRAND          516..526
FT                   /evidence="ECO:0007829|PDB:6DJ4"
FT   STRAND          528..534
FT                   /evidence="ECO:0007829|PDB:6DJ4"
FT   HELIX           556..558
FT                   /evidence="ECO:0007829|PDB:6DJ4"
FT   STRAND          560..563
FT                   /evidence="ECO:0007829|PDB:6DJ4"
FT   STRAND          571..584
FT                   /evidence="ECO:0007829|PDB:6DJ4"
FT   STRAND          590..600
FT                   /evidence="ECO:0007829|PDB:6DJ4"
SQ   SEQUENCE   1174 AA;  133622 MW;  B51E9751D7F91C61 CRC64;
     MENNIQNQCV PYNCLNNPEV EILNEERSTG RLPLDISLSL TRFLLSEFVP GVGVAFGLFD
     LIWGFITPSD WSLFLLQIEQ LIEQRIETLE RNRAITTLRG LADSYEIYIE ALREWEANPN
     NAQLREDVRI RFANTDDALI TAINNFTLTS FEIPLLSVYV QAANLHLSLL RDAVSFGQGW
     GLDIATVNNH YNRLINLIHR YTKHCLDTYN QGLENLRGTN TRQWARFNQF RRDLTLTVLD
     IVALFPNYDV RTYPIQTSSQ LTREIYTSSV IEDSPVSANI PNGFNRAEFG VRPPHLMDFM
     NSLFVTAETV RSQTVWGGHL VSSRNTAGNR INFPSYGVFN PGGAIWIADE DPRPFYRTLS
     DPVFVRGGFG NPHYVLGLRG VAFQQTGTNH TRTFRNSGTI DSLDEIPPQD NSGAPWNDYS
     HVLNHVTFVR WPGEISGSDS WRAPMFSWTH RSATPTNTID PERITQIPLV KAHTLQSGTT
     VVRGPGFTGG DILRRTSGGP FAYTIVNING QLPQRYRARI RYASTTNLRI YVTVAGERIF
     AGQFNKTMDT GDPLTFQSFS YATINTAFTF PMSQSSFTVG ADTFSSGNEV YIDRFELIPV
     TATFEAEYDL ERAQKAVNAL FTSINQIGIK TDVTDYHIDQ VSNLVDCLSD EFCLDEKREL
     SEKVKHAKRL SDERNLLQDP NFKGINRQLD RGWRGSTDIT IQRGDDVFKE NYVTLPGTFD
     ECYPTYLYQK IDESKLKPYT RYQLRGYIED SQDLEIYLIR YNAKHETVNV LGTGSLWPLS
     VQSPIRKCGE PNRCAPHLEW NPDLDCSCRD GEKCAHHSHH FSLDIDVGCT DLNEDLDVWV
     IFKIKTQDGH ARLGNLEFLE EKPLVGEALA RVKRAEKKWR DKREKLELET NIVYKEAKES
     VDALFVNSQY DQLQADTNIA MIHAADKRVH RIREAYLPEL SVIPGVNVDI FEELKGRIFT
     AFFLYDARNV IKNGDFNNGL SCWNVKGHVD VEEQNNHRSV LVVPEWEAEV SQEVRVCPGR
     GYILRVTAYK EGYGEGCVTI HEIENNTDEL KFSNCVEEEV YPNNTVTCND YTANQEEYGG
     AYTSRNRGYD ETYGSNSSVP ADYASVYEEK SYTDGRRDNP CESNRGYGDY TPLPAGYVTK
     ELEYFPETDK VWIEIGETEG TFIVDSVELL LMEE