CR1IA_BACTK
ID CR1IA_BACTK Reviewed; 719 AA.
AC Q45752; P71092; Q45750; Q45751; Q45756;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 88.
DE RecName: Full=Pesticidal crystal protein Cry1Ia;
DE AltName: Full=81 kDa crystal protein;
DE AltName: Full=Crystaline entomocidal protoxin;
DE AltName: Full=Insecticidal delta-endotoxin CryII(a);
GN Name=cry1Ia; Synonyms=CGCryV, cryII(a), cryV, cryV1;
OS Bacillus thuringiensis subsp. kurstaki.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=29339;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DSIR732;
RX PubMed=8517758; DOI=10.1128/aem.59.5.1683-1687.1993;
RA Gleave A.P., Williams R., Hedges R.J.;
RT "Screening by polymerase chain reaction of Bacillus thuringiensis serotypes
RT for the presence of cryV-like insecticidal protein genes and
RT characterization of a cryV gene cloned from B. thuringiensis subsp.
RT kurstaki.";
RL Appl. Environ. Microbiol. 59:1683-1687(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=JHCC4835;
RX PubMed=1588820; DOI=10.1111/j.1365-2958.1992.tb01560.x;
RA Tailor R., Tippett J., Gibb G., Pells S., Pike D., Jordan L., Ely S.;
RT "Identification and characterization of a novel Bacillus thuringiensis
RT delta-endotoxin entomocidal to coleopteran and lepidopteran larvae.";
RL Mol. Microbiol. 6:1211-1217(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=HD-1;
RX PubMed=7793960; DOI=10.1128/aem.61.6.2402-2407.1995;
RA Shin B.-S., Park S.-H., Choi S.-K., Koo B.T., Lee S.T., Kim J.I.;
RT "Distribution of cryV-type insecticidal protein genes in Bacillus
RT thuringiensis and cloning of cryV-type genes from Bacillus thuringiensis
RT subsp. kurstaki and Bacillus thuringiensis subsp. entomocidus.";
RL Appl. Environ. Microbiol. 61:2402-2407(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=AB88;
RX PubMed=8606196; DOI=10.1128/jb.178.7.2141-2144.1996;
RA Kostichka K., Warren G.W., Mullins M., Mullins A.D., Palekar N.V.,
RA Craig J.A., Koziel M.G., Estruch J.J.;
RT "Cloning of a cryV-type insecticidal protein gene from Bacillus
RT thuringiensis: the cryV-encoded protein is expressed early in stationary
RT phase.";
RL J. Bacteriol. 178:2141-2144(1996).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=61;
RA Selvapandiyan A., Bhatnagar R.K.;
RT "Isolation, cloning and expression of cryV gene.";
RL Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC epithelial cells of certain coleopteran and lepidopteran species.
CC Active on Plutella xylostella and Bombyx mori.
CC -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC and is accumulated both as an inclusion and as part of the spore coat.
CC -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC terminus.
CC -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR EMBL; M98544; AAA22354.1; -; Genomic_DNA.
DR EMBL; X62821; CAA44633.1; -; Genomic_DNA.
DR EMBL; L36338; AAC36999.1; -; Genomic_DNA.
DR EMBL; L49391; AAB00958.1; -; Genomic_DNA.
DR EMBL; Y08920; CAA70124.1; -; Genomic_DNA.
DR PIR; I39814; I39814.
DR PIR; I39815; I39815.
DR PIR; S25383; S25383.
DR RefSeq; WP_000769223.1; NZ_PGEH01000276.1.
DR AlphaFoldDB; Q45752; -.
DR SMR; Q45752; -.
DR GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 1.20.190.10; -; 1.
DR Gene3D; 2.100.10.10; -; 1.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR038979; Pest_crys.
DR InterPro; IPR005638; Pest_crys_C.
DR InterPro; IPR005639; Pest_crys_N.
DR InterPro; IPR036716; Pest_crys_N_sf.
DR InterPro; IPR001178; Pest_cryst_cen_dom.
DR InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR PANTHER; PTHR37003; PTHR37003; 1.
DR Pfam; PF03944; Endotoxin_C; 1.
DR Pfam; PF00555; Endotoxin_M; 1.
DR Pfam; PF03945; Endotoxin_N; 1.
DR SUPFAM; SSF49785; SSF49785; 1.
DR SUPFAM; SSF51096; SSF51096; 1.
DR SUPFAM; SSF56849; SSF56849; 1.
PE 2: Evidence at transcript level;
KW Sporulation; Toxin; Virulence.
FT CHAIN 1..719
FT /note="Pesticidal crystal protein Cry1Ia"
FT /id="PRO_0000174048"
FT VARIANT 159
FT /note="K -> R (in strain: 61)"
FT VARIANT 233
FT /note="D -> Y (in strain: JHCC4835 and HD-1)"
FT VARIANT 443
FT /note="A -> V (in strain: AB88)"
FT VARIANT 711..712
FT /note="KQ -> NE (in strain: HD-1 and 61)"
SQ SEQUENCE 719 AA; 81216 MW; 3627E5A6C25DAFF5 CRC64;
MKLKNQDKHQ SFSSNAKVDK ISTDSLKNET DIELQNINHE DCLKMSEYEN VEPFVSASTI
QTGIGIAGKI LGTLGVPFAG QVASLYSFIL GELWPKGKNQ WEIFMEHVEE IINQKISTYA
RNKALTDLKG LGDALAVYHD SLESWVGNRN NTRARSVVKS QYIALELMFV QKLPSFAVSG
EEVPLLPIYA QAANLHLLLL RDASIFGKEW GLSSSEISTF YNRQVERAGD YSDHCVKWYS
TGLNNLRGTN AESWVRYNQF RRDMTLMVLD LVALFPSYDT QMYPIKTTAQ LTREVYTDAI
GTVHPHPSFT STTWYNNNAP SFSAIEAAVV RNPHLLDFLE QVTIYSLLSR WSNTQYMNMW
GGHKLEFRTI GGTLNISTQG STNTSINPVT LPFTSRDVYR TESLAGLNLF LTQPVNGVPR
VDFHWKFVTH PIASDNFYYP GYAGIGTQLQ DSENELPPEA TGQPNYESYS HRLSHIGLIS
ASHVKALVYS WTHRSADRTN TIEPNSITQI PLVKAFNLSS GAAVVRGPGF TGGDILRRTN
TGTFGDIRVN INPPFAQRYR VRIRYASTTD LQFHTSINGK AINQGNFSAT MNRGEDLDYK
TFRTVGFTTP FSFLDVQSTF TIGAWNFSSG NEVYIDRIEF VPVEVTYEAE YDFEKAQEKV
TALFTSTNPR GLKTDVKDYH IDQVSNLVES LSDEFYLDEK RELFEIVKYA KQLHIERNM
