ID   CR21_LITPE              Reviewed;          25 AA.
AC   P86485;
DT   18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 1.
DT   25-MAY-2022, entry version 8.
DE   RecName: Full=Caerin 2.1 {ECO:0000303|PubMed:19642086};
OS   Litoria peronii (Emerald spotted tree frog) (Hyla peronii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Pelodryadinae; Litoria.
OX   NCBI_TaxID=317363;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP   MASS SPECTROMETRY.
RC   TISSUE=Skin secretion {ECO:0000269|PubMed:19642086};
RX   PubMed=19642086; DOI=10.1002/rcm.4164;
RA   Bilusich D., Jackway R.J., Musgrave I.F., Tyler M.J., Bowie J.H.;
RT   "The host-defence skin peptide profiles of Peron's Tree Frog Litoria
RT   peronii in winter and summer. Sequence determination by electrospray mass
RT   spectrometry and activities of the peptides.";
RL   Rapid Commun. Mass Spectrom. 23:2628-2636(2009).
CC   -!- FUNCTION: Antibacterial peptide with narrow spectrum of activity.
CC       Active against the Gram-negative bacterium P.multocida (MIC=25 ug/ml).
CC       Inhibits the formation of NO by neuronal nitric oxide synthase with an
CC       IC(50) of 9 ug/ml. {ECO:0000269|PubMed:19642086}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19642086}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin dorsal glands.
CC       {ECO:0000269|PubMed:19642086}.
CC   -!- MASS SPECTROMETRY: Mass=2391; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:19642086};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Caerin subfamily. {ECO:0000255}.
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DR   AlphaFoldDB; P86485; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0051001; P:negative regulation of nitric-oxide synthase activity; IDA:UniProtKB.
DR   InterPro; IPR032021; Frog_Litoria.
DR   Pfam; PF16049; Antimicrobial24; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Secreted.
FT   PEPTIDE         1..25
FT                   /note="Caerin 2.1"
FT                   /evidence="ECO:0000269|PubMed:19642086"
FT                   /id="PRO_0000394178"
SQ   SEQUENCE   25 AA;  2394 MW;  DDCA9BC6B49186B8 CRC64;
     GLVSSIGRAL GGLLADVVKS KGQPA