ID   CR2AB_BACTK             Reviewed;         633 AA.
AC   P21254;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Pesticidal crystal protein Cry2Ab;
DE   AltName: Full=71 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryIIA(b);
GN   Name=cry2Ab; Synonyms=cryB2 {ECO:0000303|PubMed:2914879}, cryIIA(b);
OS   Bacillus thuringiensis subsp. kurstaki.
OG   Plasmid unnamed.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=29339;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INSECT TOXICITY.
RC   STRAIN=HD-1;
RX   PubMed=2914879; DOI=10.1128/jb.171.2.965-974.1989;
RA   Widner W.R., Whiteley H.R.;
RT   "Two highly related insecticidal crystal proteins of Bacillus thuringiensis
RT   subsp. kurstaki possess different host range specificities.";
RL   J. Bacteriol. 171:965-974(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2089222; DOI=10.1111/j.1365-2958.1990.tb00569.x;
RA   Dankocsik C.C., Donovan W.P., Jany C.S.;
RT   "Activation of a cryptic crystal protein gene of Bacillus thuringiensis
RT   subspecies kurstaki by gene fusion and determination of the crystal protein
RT   insecticidal specificity.";
RL   Mol. Microbiol. 4:2087-2094(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BTC002;
RA   Chen Z.Y., Zhang J., Huang D.F.;
RT   "Bacillus thuringiensis Btc002 cry2Ab gene.";
RL   Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of lepidopteran (Manduca sexta) larvae.
CC       {ECO:0000269|PubMed:2914879}.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- MISCELLANEOUS: Encoded on an unnamed 225 kb plasmid.
CC       {ECO:0000269|PubMed:2914879}.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; M23724; AAA22342.1; -; Genomic_DNA.
DR   EMBL; X55416; CAA39075.1; -; Genomic_DNA.
DR   EMBL; AF164666; AAG36762.1; -; Genomic_DNA.
DR   PIR; D32053; D32053.
DR   RefSeq; WP_001089638.1; NZ_PGEH01000013.1.
DR   AlphaFoldDB; P21254; -.
DR   SMR; P21254; -.
DR   TCDB; 1.C.2.2.2; the channel-forming Delta-endotoxin insecticidal crystal protein (icp) family.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR038979; Pest_crys.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR015214; Pest_cryst_cen_dom_Cry2A/18.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   PANTHER; PTHR37003; PTHR37003; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF09131; Endotoxin_mid; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Plasmid; Sporulation; Toxin; Virulence.
FT   CHAIN           1..633
FT                   /note="Pesticidal crystal protein Cry2Ab"
FT                   /id="PRO_0000174057"
SQ   SEQUENCE   633 AA;  70741 MW;  424B937DFDDF3D61 CRC64;
     MNSVLNSGRT TICDAYNVAA HDPFSFQHKS LDTVQKEWTE WKKNNHSLYL DPIVGTVASF
     LLKKVGSLVG KRILSELRNL IFPSGSTNLM QDILRETEKF LNQRLNTDTL ARVNAELTGL
     QANVEEFNRQ VDNFLNPNRN AVPLSITSSV NTMQQLFLNR LPQFQMQGYQ LLLLPLFAQA
     ANLHLSFIRD VILNADEWGI SAATLRTYRD YLKNYTRDYS NYCINTYQSA FKGLNTRLHD
     MLEFRTYMFL NVFEYVSIWS LFKYQSLLVS SGANLYASGS GPQQTQSFTS QDWPFLYSLF
     QVNSNYVLNG FSGARLSNTF PNIVGLPGST TTHALLAARV NYSGGISSGD IGASPFNQNF
     NCSTFLPPLL TPFVRSWLDS GSDREGVATV TNWQTESFET TLGLRSGAFT ARGNSNYFPD
     YFIRNISGVP LVVRNEDLRR PLHYNEIRNI ASPSGTPGGA RAYMVSVHNR KNNIHAVHEN
     GSMIHLAPND YTGFTISPIH ATQVNNQTRT FISEKFGNQG DSLRFEQNNT TARYTLRGNG
     NSYNLYLRVS SIGNSTIRVT INGRVYTATN VNTTTNNDGV NDNGARFSDI NIGNVVASSN
     SDVPLDINVT LNSGTQFDLM NIMLVPTNIS PLY