ID   CR2AC_BACTU             Reviewed;         622 AA.
AC   Q45743;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Pesticidal crystal protein Cry2Ac;
DE   AltName: Full=70 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryIIA(c);
GN   Name=cry2Ac; Synonyms=cryIIA(c), cryIIc;
OS   Bacillus thuringiensis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1428;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Shanghai 1 / S-1;
RX   PubMed=1651878; DOI=10.1016/0378-1097(91)90466-n;
RA   Wu D., Cao X.L., Bai Y.Y., Aronson A.I.;
RT   "Sequence of an operon containing a novel delta-endotoxin gene from
RT   Bacillus thuringiensis.";
RL   FEMS Microbiol. Lett. 65:31-36(1991).
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of lepidopteran larvae. Has low activity on dipteran
CC       larvae.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X57252; CAA40536.1; -; Genomic_DNA.
DR   PIR; S17402; S17402.
DR   PIR; S42282; S42282.
DR   AlphaFoldDB; Q45743; -.
DR   SMR; Q45743; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR015214; Pest_cryst_cen_dom_Cry2A/18.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF09131; Endotoxin_mid; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Sporulation; Toxin; Virulence.
FT   CHAIN           1..622
FT                   /note="Pesticidal crystal protein Cry2Ac"
FT                   /id="PRO_0000174058"
SQ   SEQUENCE   622 AA;  69729 MW;  E0BE5FAD37BF8299 CRC64;
     MNTVLNNGRN TTCHAHNVVA HDPFSFEHKS LNTIEKEWKE WKRTDHSLYV APIVGTVGSF
     LLKKVGSLVG KRILSELQNL IFPSGSIDLM QEILRATEQF INQRLNADTL GRVNAELAGL
     QANVAEFNRQ VDNFLNPNQN PVPLAIIDSV NTLQQLFLSR LPQFQIQGYQ LLLLPLFAQA
     ANFNLSFIRG VILNADEWGI SAATVRTYRD HLRKFHRDYS NYCINPYQTA FRGLNHRLPD
     MLEFRTYMFL NVFEYVSIWS LFKYQSLLVS SGANLYASGS GPTQSFTAQN WPFLYSLFQV
     NSNYVLNGLS GARTTITFPN IGGLPVYHNS TLHFARINYR GGVSSSRIGQ ANLNQNFNIS
     TLFNPLQTPF IRSWLDSGTD REGVATSTNW QSGAFETTLL RFSIFSARGN SNFFPDYFIR
     NISGVVGTIS NADLARPLHF NEIRDIGTTA VASLVTVHNR KNNIYDTHEN GTMIHLAPND
     YTGFTVSPIH ATQVNNQIRT FISEKYGNQG DSLRFELSNP TARYTLRGNG NSYNLYLRVS
     SIGSSTIRVT INGRVYTANV NTTTNNDGVL DNGARFSDIN IGNVVASANT NVPLDIQVTF
     NGNPQFELMN IMFVPTNLPP LY