ID   CR2AD_BACTU             Reviewed;         633 AA.
AC   Q9RMG3;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Pesticidal crystal protein Cry2Ad;
DE   AltName: Full=71 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryIIA(d);
GN   Name=cry2Ad; Synonyms=cry2, cryIIA(d);
OS   Bacillus thuringiensis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1428;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BR30;
RA   Choi S.-K., Shin B.-S., Park S.-H.;
RT   "Nucleotide sequence of a new Bacillus thuringiensis cry2-type gene.";
RL   Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of insects.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; AF200816; AAF09583.1; -; Genomic_DNA.
DR   RefSeq; WP_001089637.1; NZ_CP014853.1.
DR   AlphaFoldDB; Q9RMG3; -.
DR   SMR; Q9RMG3; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR038979; Pest_crys.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR015214; Pest_cryst_cen_dom_Cry2A/18.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   PANTHER; PTHR37003; PTHR37003; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF09131; Endotoxin_mid; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Sporulation; Toxin; Virulence.
FT   CHAIN           1..633
FT                   /note="Pesticidal crystal protein Cry2Ad"
FT                   /id="PRO_0000174059"
SQ   SEQUENCE   633 AA;  70752 MW;  2A582067131B39CB CRC64;
     MNSVLNSGRN TICDAYNVVV HDPFSFQHKS LDTIQKEWME WKKDNHSLYV DPIVGTVASF
     LLKKLGSLIG KRILSELRNL IFPSGSTNLM EDILRETEKF LNQKLNTDTL SRVNAELTGL
     QANVEEFNRQ VDNFLNPNRN AVPLSITSSV NTMQQLFLNR LSQFQMQGYQ LLLLPLFAQA
     ANLHLSFIRD VILNAEEWGI SAATLRTYQN HLRNYTRDYS NYCIDTYQTA FRGLNTRLHD
     MLEFRTYMFL NVFEYVSIWS LFKYQSLLVS SGANLYASGS GPQQTQLFTS QDWPFLYSLF
     QVNSNYVLSG FSGASLFTTF PNIGGLPGST TTQALLAARV NYSGGITSGS IGGSNFNQNF
     NCNTISPPLS TSFVRSWLDS GSDRQGVNTV TNWQTESFET TSGLRCGAFT PRGNSNYYPG
     YFIRNISGVS LVLRNEDLKR PLYYNEKRNI ESPSGTPGGA RAYMVSVHNK KNNIYAVHEN
     GTMIHLAPED NTGFTISPIH ATQVNNQTRT FISEKFGNQG DSLRFEQSNT TARYTLRGNG
     NSYNLYLRVS SIGNSTIRVT INGRVYTASN VNTTTNNDGV NDNGARFSDI NIGNVVASSN
     SDVPLDINVT LNSGTQFDLM NIMLVPTNIS PLY