CR3AA_BACTD
ID CR3AA_BACTD Reviewed; 644 AA.
AC P0A381; P07130; P21255;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1988, sequence version 1.
DT 25-MAY-2022, entry version 56.
DE RecName: Full=Pesticidal crystal protein Cry3Aa;
DE AltName: Full=73 kDa crystal protein;
DE AltName: Full=Crystaline entomocidal protoxin;
DE AltName: Full=Insecticidal delta-endotoxin CryIIIA(a);
DE Flags: Precursor;
GN Name=cry3Aa; Synonyms=bt13, cry3A, cryC, cryIIIa, cryIIIA(a);
OS Bacillus thuringiensis subsp. san diego.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=1435;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2828180; DOI=10.1016/0378-1119(87)90174-0;
RA Herrnstadt C., Gilroy T.E., Sobieski D.A., Bennett B.D., Gaertner F.H.;
RT "Nucleotide sequence and deduced amino acid sequence of a coleopteran-
RT active delta-endotoxin gene from Bacillus thuringiensis subsp. san diego.";
RL Gene 57:37-46(1987).
CC -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC epithelial cells of Coleoptera.
CC -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC and is accumulated both as an inclusion and as part of the spore coat.
CC -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC terminus.
CC -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA22336.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M22472; AAA22336.1; ALT_INIT; Genomic_DNA.
DR PDB; 6LFP; X-ray; 3.31 A; A=1-644.
DR PDBsum; 6LFP; -.
DR AlphaFoldDB; P0A381; -.
DR SMR; P0A381; -.
DR GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 1.20.190.10; -; 1.
DR Gene3D; 2.100.10.10; -; 1.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR038979; Pest_crys.
DR InterPro; IPR005638; Pest_crys_C.
DR InterPro; IPR005639; Pest_crys_N.
DR InterPro; IPR036716; Pest_crys_N_sf.
DR InterPro; IPR001178; Pest_cryst_cen_dom.
DR InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR PANTHER; PTHR37003; PTHR37003; 1.
DR Pfam; PF03944; Endotoxin_C; 1.
DR Pfam; PF00555; Endotoxin_M; 1.
DR Pfam; PF03945; Endotoxin_N; 1.
DR SUPFAM; SSF49785; SSF49785; 1.
DR SUPFAM; SSF51096; SSF51096; 1.
DR SUPFAM; SSF56849; SSF56849; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Sporulation; Toxin; Virulence.
FT PROPEP 1..57
FT /note="Removed in mature form"
FT /id="PRO_0000006341"
FT CHAIN 58..644
FT /note="Pesticidal crystal protein Cry3Aa"
FT /id="PRO_0000006342"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 64..78
FT /evidence="ECO:0007829|PDB:6LFP"
FT HELIX 85..98
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 100..103
FT /evidence="ECO:0007829|PDB:6LFP"
FT HELIX 104..116
FT /evidence="ECO:0007829|PDB:6LFP"
FT HELIX 123..152
FT /evidence="ECO:0007829|PDB:6LFP"
FT TURN 155..157
FT /evidence="ECO:0007829|PDB:6LFP"
FT HELIX 160..180
FT /evidence="ECO:0007829|PDB:6LFP"
FT HELIX 181..184
FT /evidence="ECO:0007829|PDB:6LFP"
FT TURN 190..193
FT /evidence="ECO:0007829|PDB:6LFP"
FT HELIX 194..214
FT /evidence="ECO:0007829|PDB:6LFP"
FT TURN 215..219
FT /evidence="ECO:0007829|PDB:6LFP"
FT HELIX 222..252
FT /evidence="ECO:0007829|PDB:6LFP"
FT HELIX 259..275
FT /evidence="ECO:0007829|PDB:6LFP"
FT HELIX 277..280
FT /evidence="ECO:0007829|PDB:6LFP"
FT HELIX 281..285
FT /evidence="ECO:0007829|PDB:6LFP"
FT TURN 288..290
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 293..296
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 302..304
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 310..312
FT /evidence="ECO:0007829|PDB:6LFP"
FT HELIX 321..325
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 335..347
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 350..352
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 356..371
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 390..393
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 398..411
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 414..428
FT /evidence="ECO:0007829|PDB:6LFP"
FT TURN 429..432
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 433..440
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 448..451
FT /evidence="ECO:0007829|PDB:6LFP"
FT HELIX 452..455
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 461..463
FT /evidence="ECO:0007829|PDB:6LFP"
FT HELIX 465..468
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 471..480
FT /evidence="ECO:0007829|PDB:6LFP"
FT HELIX 482..484
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 487..495
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 506..513
FT /evidence="ECO:0007829|PDB:6LFP"
FT HELIX 514..516
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 518..520
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 525..527
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 531..535
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 537..540
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 542..550
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 552..554
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 560..571
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 573..579
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 582..589
FT /evidence="ECO:0007829|PDB:6LFP"
FT HELIX 601..603
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 605..608
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 618..625
FT /evidence="ECO:0007829|PDB:6LFP"
FT STRAND 633..643
FT /evidence="ECO:0007829|PDB:6LFP"
SQ SEQUENCE 644 AA; 73109 MW; EFCFF1CEEFDA9683 CRC64;
MNPNNRSEHD TIKTTENNEV PTNHVQYPLA ETPNPTLEDL NYKEFLRMTA DNNTEALDSS
TTKDVIQKGI SVVGDLLGVV GFPFGGALVS FYTNFLNTIW PSEDPWKAFM EQVEALMDQK
IADYAKNKAL AELQGLQNNV EDYVSALSSW QKNPVSSRNP HSQGRIRELF SQAESHFRNS
MPSFAISGYE VLFLTTYAQA ANTHLFLLKD AQIYGEEWGY EKEDIAEFYK RQLKLTQEYT
DHCVKWYNVG LDKLRGSSYE SWVNFNRYRR EMTLTVLDLI ALFPLYDVRL YPKEVKTELT
RDVLTDPIVG VNNLRGYGTT FSNIENYIRK PHLFDYLHRI QFHTRFQPGY YGNDSFNYWS
GNYVSTRPSI GSNDIITSPF YGNKSSEPVQ NLEFNGEKVY RAVANTNLAV WPSAVYSGVT
KVEFSQYNDQ TDEASTQTYD SKRNVGAVSW DSIDQLPPET TDEPLEKGYS HQLNYVMCFL
MQGSRGTIPV LTWTHKSVDF FNMIDSKKIT QLPLVKAYKL QSGASVVAGP RFTGGDIIQC
TENGSAATIY VTPDVSYSQK YRARIHYAST SQITFTLSLD GAPFNQYYFD KTINKGDTLT
YNSFNLASFS TPFELSGNNL QIGVTGLSAG DKVYIDKIEF IPVN
