CR3BB_BACTU
ID CR3BB_BACTU Reviewed; 652 AA.
AC Q06117; Q45717;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=Pesticidal crystal protein Cry3Bb;
DE AltName: Full=74 kDa crystal protein;
DE AltName: Full=Crystaline entomocidal protoxin;
DE AltName: Full=Insecticidal delta-endotoxin CryIIIB(b);
GN Name=cry3Bb; Synonyms=cryIIIB(b), cryIIIb2;
OS Bacillus thuringiensis.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=1428;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=EG4961;
RX PubMed=1476436; DOI=10.1128/aem.58.12.3921-3927.1992;
RA Donovan W.P., Rupar M.J., Slaney A.C., Malvar T., Gawron-Burke M.C.,
RA Johnson T.B.;
RT "Characterization of two genes encoding Bacillus thuringiensis insecticidal
RT crystal proteins toxic to Coleoptera species.";
RL Appl. Environ. Microbiol. 58:3921-3927(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=NRRL B-18655 / EG5144;
RA Donovan W.P., Rupar M.J., Slaney A.C.;
RT "Bacillus thuringiensis cryIIIC, (b) protein toxic to coleopteran
RT insects.";
RL Patent number US5378625, 03-JAN-1995.
CC -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC epithelial cells of Coleoptera. Has moderate level of toxicity to
CC southern corn rootworm.
CC -!- SUBUNIT: Monomer.
CC -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC and is accumulated both as an inclusion and as part of the spore coat.
CC -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC terminus.
CC -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR EMBL; M89794; AAA22334.1; -; Genomic_DNA.
DR EMBL; U31633; AAA74198.1; -; Genomic_DNA.
DR PIR; I39811; I39811.
DR PDB; 1JI6; X-ray; 2.40 A; A=64-652.
DR PDBsum; 1JI6; -.
DR AlphaFoldDB; Q06117; -.
DR SMR; Q06117; -.
DR EvolutionaryTrace; Q06117; -.
DR GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 1.20.190.10; -; 1.
DR Gene3D; 2.100.10.10; -; 1.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR038979; Pest_crys.
DR InterPro; IPR005638; Pest_crys_C.
DR InterPro; IPR005639; Pest_crys_N.
DR InterPro; IPR036716; Pest_crys_N_sf.
DR InterPro; IPR001178; Pest_cryst_cen_dom.
DR InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR PANTHER; PTHR37003; PTHR37003; 1.
DR Pfam; PF03944; Endotoxin_C; 1.
DR Pfam; PF00555; Endotoxin_M; 1.
DR Pfam; PF03945; Endotoxin_N; 1.
DR SUPFAM; SSF49785; SSF49785; 1.
DR SUPFAM; SSF51096; SSF51096; 1.
DR SUPFAM; SSF56849; SSF56849; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Sporulation; Toxin; Virulence.
FT CHAIN 1..652
FT /note="Pesticidal crystal protein Cry3Bb"
FT /id="PRO_0000174061"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 433..465
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 433..460
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 21
FT /note="Q -> P (in strain: EG5144)"
FT VARIANT 97
FT /note="N -> D (in strain: EG5144)"
FT VARIANT 289
FT /note="I -> V (in strain: EG5144)"
FT VARIANT 352
FT /note="F -> S (in strain: EG5144)"
FT VARIANT 417..419
FT /note="VYL -> IYF (in strain: EG5144)"
FT VARIANT 451
FT /note="S -> G (in strain: EG5144)"
FT VARIANT 590
FT /note="L -> I (in strain: EG5144)"
FT VARIANT 600
FT /note="K -> I (in strain: EG5144)"
FT VARIANT 624
FT /note="K -> T (in strain: EG5144)"
FT HELIX 65..79
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 83..86
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 91..98
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 105..113
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 115..118
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 124..153
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 156..158
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 161..181
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 182..185
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 191..209
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 211..215
FT /evidence="ECO:0007829|PDB:1JI6"
FT TURN 216..220
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 223..254
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 260..276
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 278..281
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 284..287
FT /evidence="ECO:0007829|PDB:1JI6"
FT TURN 289..294
FT /evidence="ECO:0007829|PDB:1JI6"
FT TURN 313..315
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 316..318
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 322..326
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 336..348
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 357..370
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 391..394
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 399..411
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 417..430
FT /evidence="ECO:0007829|PDB:1JI6"
FT TURN 431..434
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 435..442
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 450..454
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 455..458
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 464..466
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 468..471
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 474..483
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 490..498
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 509..516
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 517..519
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 521..523
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 527..530
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 534..538
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 540..545
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 550..556
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 559..562
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 566..576
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 578..584
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 589..594
FT /evidence="ECO:0007829|PDB:1JI6"
FT HELIX 606..608
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 610..613
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 625..631
FT /evidence="ECO:0007829|PDB:1JI6"
FT STRAND 640..649
FT /evidence="ECO:0007829|PDB:1JI6"
SQ SEQUENCE 652 AA; 74385 MW; 63048332CDE8CCC6 CRC64;
MNPNNRSEHD TIKVTPNSEL QTNHNQYPLA DNPNSTLEEL NYKEFLRMTE DSSTEVLDNS
TVKDAVGTGI SVVGQILGVV GVPFAGALTS FYQSFLNTIW PSDADPWKAF MAQVEVLIDK
KIEEYAKSKA LAELQGLQNN FEDYVNALNS WKKTPLSLRS KRSQDRIREL FSQAESHFRN
SMPSFAVSKF EVLFLPTYAQ AANTHLLLLK DAQVFGEEWG YSSEDVAEFY HRQLKLTQQY
TDHCVNWYNV GLNGLRGSTY DAWVKFNRFR REMTLTVLDL IVLFPFYDIR LYSKGVKTEL
TRDIFTDPIF SLNTLQEYGP TFLSIENSIR KPHLFDYLQG IEFHTRLQPG YFGKDSFNYW
SGNYVETRPS IGSSKTITSP FYGDKSTEPV QKLSFDGQKV YRTIANTDVA AWPNGKVYLG
VTKVDFSQYD DQKNETSTQT YDSKRNNGHV SAQDSIDQLP PETTDEPLEK AYSHQLNYAE
CFLMQDRRGT IPFFTWTHRS VDFFNTIDAE KITQLPVVKA YALSSGASII EGPGFTGGNL
LFLKESSNSI AKFKVTLNSA ALLQRYRVRI RYASTTNLRL FVQNSNNDFL VIYINKTMNK
DDDLTYQTFD LATTNSNMGF SGDKNELIIG AESFVSNEKI YIDKIEFIPV QL
