CR4BA_BACTI
ID CR4BA_BACTI Reviewed; 1136 AA.
AC P05519; P11782; P16479;
DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 25-MAY-2022, entry version 119.
DE RecName: Full=Pesticidal crystal protein Cry4Ba;
DE AltName: Full=128 kDa crystal protein;
DE AltName: Full=Crystaline entomocidal protoxin;
DE AltName: Full=Insecticidal delta-endotoxin CryIVB(a);
GN Name=cry4Ba; Synonyms=bt8, cryD2, cryIVB(a), isrH3;
OS Bacillus thuringiensis subsp. israelensis.
OG Plasmid 72 Kb.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=1430;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2831510; DOI=10.1093/nar/16.4.1637;
RA Tungpradubkul S., Settasatien C., Panyim S.;
RT "The complete nucleotide sequence of a 130 kDa mosquito-larvicidal delta-
RT endotoxin gene of Bacillus thuringiensis var. israelensis.";
RL Nucleic Acids Res. 16:1637-1638(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=4Q2-72;
RX PubMed=2833395; DOI=10.1111/j.1432-1033.1988.tb13960.x;
RA Chungjatupornchai W., Hoefte H., Seurinck J., Angsuthanasombat C.,
RA Vaeck M.;
RT "Common features of Bacillus thuringiensis toxins specific for Diptera and
RT Lepidoptera.";
RL Eur. J. Biochem. 173:9-16(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Sen K., Honda G., Koyama N., Nishida M., Neki A., Sakai H., Himeno M.,
RA Komano T.;
RT "Cloning and nucleotide sequences of the two 130 kDa insecticidal protein
RT genes of Bacillus thuringiensis var. israelensis.";
RL Agric. Biol. Chem. 52:873-878(1988).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2901387; DOI=10.1016/0378-1119(88)90229-6;
RA Yamamoto T., Watkinson I.A., Kim L., Sage M.V., Stratton R., Akande N.,
RA Li Y., Ma D.-P., Roe B.A.;
RT "Nucleotide sequence of the gene coding for a 130-kDa mosquitocidal protein
RT of Bacillus thuringiensis israelensis.";
RL Gene 66:107-120(1988).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-134.
RX PubMed=2890080; DOI=10.1007/bf00328128;
RA Angsuthanasombat C., Chungjatupornchai W., Kertbundit S., Luxananil P.,
RA Settasatian C., Wilairat P., Panyim S.;
RT "Cloning and expression of 130-kd mosquito-larvicidal delta-endotoxin gene
RT of Bacillus thuringiensis var. israelensis in Escherichia coli.";
RL Mol. Gen. Genet. 208:384-389(1987).
CC -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC epithelial cells of mosquitos.
CC -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC and is accumulated both as an inclusion and as part of the spore coat.
CC -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC terminus.
CC -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR EMBL; X07082; CAA30114.1; -; Genomic_DNA.
DR EMBL; X05692; CAA29174.1; -; Genomic_DNA.
DR EMBL; D00247; BAA00178.1; -; Genomic_DNA.
DR EMBL; M20242; AAA22337.1; -; Genomic_DNA.
DR EMBL; X07423; CAA30312.1; -; Genomic_DNA.
DR PIR; S00398; USBS8I.
DR RefSeq; WP_012211099.1; NZ_KY352353.1.
DR PDB; 1W99; X-ray; 1.75 A; A=84-641.
DR PDB; 4MOA; X-ray; 2.00 A; A=40-641.
DR PDBsum; 1W99; -.
DR PDBsum; 4MOA; -.
DR AlphaFoldDB; P05519; -.
DR SMR; P05519; -.
DR TCDB; 1.C.2.1.2; the channel-forming Delta-endotoxin insecticidal crystal protein (icp) family.
DR EvolutionaryTrace; P05519; -.
DR GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 1.20.190.10; -; 1.
DR Gene3D; 2.100.10.10; -; 1.
DR InterPro; IPR041587; Cry_V.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR038979; Pest_crys.
DR InterPro; IPR005638; Pest_crys_C.
DR InterPro; IPR005639; Pest_crys_N.
DR InterPro; IPR036716; Pest_crys_N_sf.
DR InterPro; IPR001178; Pest_cryst_cen_dom.
DR InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR PANTHER; PTHR37003; PTHR37003; 1.
DR Pfam; PF17997; Cry1Ac_D5; 1.
DR Pfam; PF03944; Endotoxin_C; 1.
DR Pfam; PF00555; Endotoxin_M; 1.
DR Pfam; PF03945; Endotoxin_N; 1.
DR SUPFAM; SSF49785; SSF49785; 2.
DR SUPFAM; SSF51096; SSF51096; 1.
DR SUPFAM; SSF56849; SSF56849; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Plasmid; Sporulation; Toxin; Virulence.
FT CHAIN 1..1136
FT /note="Pesticidal crystal protein Cry4Ba"
FT /id="PRO_0000174065"
FT CONFLICT 51
FT /note="V -> D (in Ref. 4; AAA22337)"
FT /evidence="ECO:0000305"
FT CONFLICT 65
FT /note="T -> S (in Ref. 4; AAA22337)"
FT /evidence="ECO:0000305"
FT CONFLICT 193..201
FT /note="LINAQEWSL -> PHKCTRMVY (in Ref. 4; AAA22337)"
FT /evidence="ECO:0000305"
FT CONFLICT 203..204
FT /note="RS -> C (in Ref. 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 205..206
FT /note="AG -> C (in Ref. 4; AAA22337)"
FT /evidence="ECO:0000305"
FT CONFLICT 205
FT /note="A -> R (in Ref. 1; CAA30114)"
FT /evidence="ECO:0000305"
FT CONFLICT 272
FT /note="Y -> L (in Ref. 3; BAA00178)"
FT /evidence="ECO:0000305"
FT CONFLICT 325
FT /note="D -> Y (in Ref. 3; BAA00178)"
FT /evidence="ECO:0000305"
FT CONFLICT 364..401
FT /note="FGSNLTHQIQLNSNVYKTSITDTSSPSNRVTKMDFYKI -> LVQIYLIKFN
FT LILIVIKLLSQILAPPLIELQKWISTKF (in Ref. 4; AAA22337)"
FT /evidence="ECO:0000305"
FT CONFLICT 467
FT /note="K -> N (in Ref. 4; AAA22337)"
FT /evidence="ECO:0000305"
FT CONFLICT 496
FT /note="K -> R (in Ref. 4; AAA22337)"
FT /evidence="ECO:0000305"
FT CONFLICT 519
FT /note="G -> GG (in Ref. 4; AAA22337)"
FT /evidence="ECO:0000305"
FT CONFLICT 551..572
FT /note="LNVSYVLQGVSRGTTISTESTF -> IECDHMYYKEFLEEQRLVQNYV (in
FT Ref. 4; AAA22337)"
FT /evidence="ECO:0000305"
FT CONFLICT 594
FT /note="F -> N (in Ref. 4; AAA22337)"
FT /evidence="ECO:0000305"
FT CONFLICT 687..690
FT /note="ELYP -> GIIS (in Ref. 4; AAA22337)"
FT /evidence="ECO:0000305"
FT CONFLICT 721
FT /note="A -> R (in Ref. 4; AAA22337)"
FT /evidence="ECO:0000305"
FT CONFLICT 823..832
FT /note="SNRCETSAVP -> LIVVSVRCA (in Ref. 4; AAA22337)"
FT /evidence="ECO:0000305"
FT CONFLICT 836..837
FT /note="GN -> WD (in Ref. 4; AAA22337)"
FT /evidence="ECO:0000305"
FT CONFLICT 902
FT /note="E -> R (in Ref. 4; AAA22337)"
FT /evidence="ECO:0000305"
FT CONFLICT 1015
FT /note="G -> V (in Ref. 4; AAA22337)"
FT /evidence="ECO:0000305"
FT TURN 42..44
FT /evidence="ECO:0007829|PDB:4MOA"
FT STRAND 45..51
FT /evidence="ECO:0007829|PDB:4MOA"
FT STRAND 56..58
FT /evidence="ECO:0007829|PDB:4MOA"
FT STRAND 62..64
FT /evidence="ECO:0007829|PDB:4MOA"
FT STRAND 68..70
FT /evidence="ECO:0007829|PDB:4MOA"
FT HELIX 74..77
FT /evidence="ECO:0007829|PDB:4MOA"
FT STRAND 78..81
FT /evidence="ECO:0007829|PDB:4MOA"
FT HELIX 99..135
FT /evidence="ECO:0007829|PDB:1W99"
FT HELIX 140..165
FT /evidence="ECO:0007829|PDB:1W99"
FT TURN 171..174
FT /evidence="ECO:0007829|PDB:1W99"
FT HELIX 175..194
FT /evidence="ECO:0007829|PDB:1W99"
FT HELIX 196..199
FT /evidence="ECO:0007829|PDB:1W99"
FT HELIX 206..236
FT /evidence="ECO:0007829|PDB:1W99"
FT HELIX 243..256
FT /evidence="ECO:0007829|PDB:1W99"
FT HELIX 258..261
FT /evidence="ECO:0007829|PDB:1W99"
FT HELIX 262..267
FT /evidence="ECO:0007829|PDB:1W99"
FT TURN 269..271
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 290..295
FT /evidence="ECO:0007829|PDB:1W99"
FT HELIX 304..311
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 319..329
FT /evidence="ECO:0007829|PDB:1W99"
FT HELIX 333..335
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 338..347
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 366..373
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 378..386
FT /evidence="ECO:0007829|PDB:1W99"
FT TURN 387..390
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 393..400
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 405..409
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 417..425
FT /evidence="ECO:0007829|PDB:1W99"
FT TURN 438..440
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 443..454
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 457..466
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 477..479
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 481..484
FT /evidence="ECO:0007829|PDB:1W99"
FT HELIX 485..487
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 489..491
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 495..498
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 502..506
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 508..511
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 513..516
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 521..527
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 531..533
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 535..547
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 550..557
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 560..567
FT /evidence="ECO:0007829|PDB:1W99"
FT HELIX 575..577
FT /evidence="ECO:0007829|PDB:1W99"
FT HELIX 585..587
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 589..594
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 596..598
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 600..602
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 607..616
FT /evidence="ECO:0007829|PDB:1W99"
FT STRAND 623..632
FT /evidence="ECO:0007829|PDB:1W99"
FT HELIX 635..640
FT /evidence="ECO:0007829|PDB:1W99"
SQ SEQUENCE 1136 AA; 127764 MW; 8AC4E8C26FE3E9B5 CRC64;
MNSGYPLAND LQGSMKNTNY KDWLAMCENN QQYGVNPAAI NSSSVSTALK VAGAILKFVN
PPAGTVLTVL SAVLPILWPT NTPTPERVWN DFMTNTGNLI DQTVTAYVRT DANAKMTVVK
DYLDQYTTKF NTWKREPNNQ SYRTAVITQF NLTSAKLRET AVYFSNLVGY ELLLLPIYAQ
VANFNLLLIR DGLINAQEWS LARSAGDQLY NTMVQYTKEY IAHSITWYNK GLDVLRNKSN
GQWITFNDYK REMTIQVLDI LALFASYDPR RYPADKIDNT KLSKTEFTRE IYTALVESPS
SKSIAALEAA LTRDVHLFTW LKRVDFWTNT IYQDLRFLSA NKIGFSYTNS SAMQESGIYG
SSGFGSNLTH QIQLNSNVYK TSITDTSSPS NRVTKMDFYK IDGTLASYNS NITPTPEGLR
TTFFGFSTNE NTPNQPTVND YTHILSYIKT DVIDYNSNRV SFAWTHKIVD PNNQIYTDAI
TQVPAVKSNF LNATAKVIKG PGHTGGDLVA LTSNGTLSGR MEIQCKTSIF NDPTRSYGLR
IRYAANSPIV LNVSYVLQGV SRGTTISTES TFSRPNNIIP TDLKYEEFRY KDPFDAIVPM
RLSSNQLITI AIQPLNMTSN NQVIIDRIEI IPITQSVLDE TENQNLESER EVVNALFTND
AKDALNIGTT DYDIDQAANL VECISEELYP KEKMLLLDEV KNAKQLSQSR NVLQNGDFES
ATLGWTTSDN ITIQEDDPIF KGHYLHMSGA RDIDGTIFPT YIFQKIDESK LKPYTRYLVR
GFVGSSKDVE LVVSRYGEEI DAIMNVPADL NYLYPSTFDC EGSNRCETSA VPANIGNTSD
MLYSCQYDTG KKHVVCQDSH QFSFTIDTGA LDTNENIGVW VMFKISSPDG YASLDNLEVI
EEGPIDGEAL SRVKHMEKKW NDQMEAKRSE TQQAYDVAKQ AIDALFTNVQ DEALQFDTTL
AQIQYAEYLV QSIPYVYNDW LSDVPGMNYD IYVELDARVA QARYLYDTRN IIKNGDFTQG
VMGWHVTGNA DVQQIDGVSV LVLSNWSAGV SQNVHLQHNH GYVLRVIAKK EGPGNGYVTL
MDCEENQEKL TFTSCEEGYI TKTVDVFPDT DRVRIEIGET EGSFYIESIE LICMNE
