ID   CR5AA_BACUD             Reviewed;        1385 AA.
AC   Q45760;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Pesticidal crystal protein Cry5Aa;
DE   AltName: Full=152 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryVA(a);
GN   Name=cry5Aa; Synonyms=cryVA, cryVA(a);
OS   Bacillus thuringiensis subsp. darmstadiensis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=132264;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NRRL B-18243 / PS17;
RA   Sick A.J., Schwab G.E., Payne J.M.;
RT   "Genes encoding nematode-active toxins cloned from Bacillus thuringiensis
RT   isolate PS17.";
RL   Patent number US5281530, 25-JAN-1994.
CC   -!- FUNCTION: Endotoxin with nematicidal activity.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; L07025; AAA67694.1; -; Genomic_DNA.
DR   PIR; T18213; T18213.
DR   AlphaFoldDB; Q45760; -.
DR   SMR; Q45760; -.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Sporulation; Toxin; Virulence.
FT   CHAIN           1..1385
FT                   /note="Pesticidal crystal protein Cry5Aa"
FT                   /id="PRO_0000174066"
FT   REGION          768..799
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1359..1385
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        768..785
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1363..1385
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1385 AA;  152440 MW;  E29FF11FC799DE95 CRC64;
     MAILNELYPS VPYNVLAYTP PSFLPDAGTQ ATPADLTAYE QLLKNLEKGI NAGTYSKAIA
     DVLKGIFIDD TINYQTYVNI GLSLITLAVP EIGIFTPFIG LFFAALNKHD APPPPNAKDI
     FEAMKPAIQE MIDRTLTADE QTFLNGEISG LQNLAARYQS TMDDIQSHGG FNKVDSGLIK
     KFTDEVLSLN SFYTDRLPVF ITDNTADRTL LGLPYYAILA SMHLMLLRDI ITKGPTWDSK
     INFTPDAIDS FKTDIKNNIK LYSKTIYDVF QKGLASYGTP SDLESFAKKQ KYIEIMTTHC
     LDFARLFPTF DPDLYPTGSG DISLQKTRRI LSPFIPIRTA DGLTLNNTSI DTSNWPNYEN
     GNGAFPNPKE RILKQFKLYP SWRAGQYGGL LQPYLWAIEV QDSVETRLYG QLPAVDPQAG
     PNYVSIDSSN PIIQINMDTW KTPPQGASGW NTNLMRGSVS GLSFLQRDGT RLSAGMGGGF
     ADTIYSLPAT HYLSYLYGTP YQTSDNYSGH VGALVGVSTP QEATLPNIIG QPDEQGNVST
     MGFPFEKASY GGTVVKEWLN GANAMKLSPG QSIGIPITNV TSGEYQIRCR YASNDNTNVF
     FNVDTGGANP IFQQINFAST VDNNTGVQGA NGVYVVKSIA TTDNSFTEIP AKTINVHLTN
     QGSSDVFLDR IEFIPFSLPL IYHGSYNTSS GADDVLWSSS NMNYYDIIVN GQANSSSIAS
     SMHLLNKGKV IKTIDIPGHS ETFFATFPVP EGFNEVRILA GLPEVSGNIT VQSNNPPQPS
     NNGGGDGGGN GGGDGGQYNF SLSGSDHTTI YHGKLETGIH VQGNYTYTGT PVLILNAYRN
     NTVVSSIPVY SPFDITIQTE ADSLELELQP RYGFATVNGT ATVKSPNVNY DRSFKLPIDL
     QNITTQVNAL FASGTQNMLA HNVSDHDIEE VVLKVDALSD EVFGDEKKAL RKLVNQAKRL
     SRARNLLIGG SFENWDAWYK GRNVVTVSDH ELFKSDHVLL PPPGLSPSYI FQKVEESKLK
     PNTRYIVSGF IAHGKDLEIV VSRYGQEVQK VVQVPYGEAF PLTSNGPVCC PPRSTSNGTL
     GDPHFFSYSI DVGALDLQAN PGIEFGLRIV NPTGMARVSN LEIREDRPLA ANEIRQVQRV
     ARNWRTEYEK ERAEVTSLIQ PVINRINGLY ENGNWNGSIR SDISYQNIDA IVLPTLPKLR
     HWFMSDRFSE QGDIMAKFQG ALNRAYAQLE QSTLLHNGHF TKDAANWTIE GDAHQITLED
     GRRVLRLPDW SSSVSQMIEI ENFNPDKEYN LVFHGQGEGT VTLEHGEETK YIETHTHHFA
     NFTTSQRQGL TFESNKVTVT ISSEDGEFLV DNIALVEAPL PTDDQNSEGN TASSTNSDTS
     MNNNQ