ID   CR5AB_BACUD             Reviewed;        1289 AA.
AC   Q45753;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Pesticidal crystal protein Cry5Ab;
DE   AltName: Full=142 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryVA(b);
GN   Name=cry5Ab; Synonyms=cryVA, cryVA(b);
OS   Bacillus thuringiensis subsp. darmstadiensis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=132264;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-15.
RC   STRAIN=NRRL B-18243 / PS17;
RA   Narva K.E., Payne J.M., Schwab G.E., Hickle L.A., Galasan T., Sick A.J.;
RT   "Novel Bacillus thuringiensis microbes active against nematodes, and genes
RT   encoding novel nematode-active toxins cloned from Bacillus thuringiensis
RT   isolates.";
RL   Patent number EP0462721, 27-DEC-1991.
CC   -!- FUNCTION: Endotoxin with nematicidal activity.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; L07026; AAA67693.1; -; Genomic_DNA.
DR   PIR; T18212; T18212.
DR   AlphaFoldDB; Q45753; -.
DR   SMR; Q45753; -.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 2.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Sporulation; Toxin; Virulence.
FT   CHAIN           1..1289
FT                   /note="Pesticidal crystal protein Cry5Ab"
FT                   /id="PRO_0000174067"
FT   REGION          1263..1289
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1267..1289
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1289 AA;  141984 MW;  F1B1C8C38CD51014 CRC64;
     MAILNELYPS VPYNVLAYTP PSFLPDAGTQ ATPADLTAYE QLLKNLEKGI NAGTYSKAIA
     DVLKGIFIDD TINYQTYVNI GLSLITLAVP EIGIFTPFIG LFFAALNKHD APPPPNAKDI
     FEAMKPAIQE MIDRTLTADE QTFLNGEISG LQNLAARYQS TMDDIQSHGG FNKVDSGLIK
     KFTDEVLSLN SFYTDRLPVF ITDNTADRTL LGLPYYAILA SMHLMLLRDI ITKGPTWDSK
     INFTPDAIDS FKTDIKNNIK LYSKTIYDVF QKGLASYGTP SDLESFAKKQ KYIEIMTTHC
     LDFARLFPTF DPDLYPTGSG DISLQKTRRI LSPFIPIRTA DGLTLNNTSI DTSNWPNYEN
     GNGAFPNPKE RILKQFKLYP SWRAAQYGGL LQPYLWAIEV QDSVETRLYG QLPAVDPQAG
     PNYVSIDSSN PIIQINMDTW KTPPQGASGW NTNLMRGSVS GLSFLQRDGT RLSAGMGGGF
     ADTIYSLPAT HYLSYLYGTP YQTSDNYSGH VGALVGVSTP QEATLPNIIG QPDEQGNVST
     MGFPFEKASY GGTVVKEWLN GANAMKLSPG QSIGIPITNV TSGEYQIRCR YASNDNTNVF
     FNVDTGGANP IFQQINFAST VDNNTGVQGA NGVYVVKSIA TTDNSFTVKI PAKTINVHLT
     NQGSSDVFLD RIEFVPILES NTVTIFNNSY TTGSANLIPA IAPLWSTSSD KALTGSMSIT
     GRTTPNSDDA LLRFFKTNYD TQTIPIPGSG KDFTNTLEIQ DIVSIDIFVG SGLHGSDGSI
     KLDFTNNNSG SGGSPKSFTE QNDLENITTQ VNALFTSNTQ DALATDVSDH DIEEVVLKVD
     ALSDEVFGKE KKTLRKFVNQ AKRLSKARNL LVGGNFDNLD AWYRGRNVVN VSNHELLKSD
     HVLLPPPGLS PSYIFQKVEE SKLKRNTRYT VSGFIAHATD LEIVVSRYGQ EIKKVVQVPY
     GEAFPLTSSG PVCCIPHSTS NGTLGNPHFF SYSIDVGALD VDTNPGIEFG LRIVNPTGMA
     RVSNLEIRED RPLAANEIRQ VQRVARNWRT EYEKERAEVT SLIQPVINRI NGLYDNGNWN
     GSIRSDISYQ NIDAIVLPTL PKLRHWFMSD RFSEQGDIMA KFQGALNRAY AQLEQNTLLH
     NGHFTKDAAN WTVEGDAHQV VLEDGKRVLR LPDWSSSVSQ TIEIENFDPD KEYQLVFHGQ
     GEGTVTLEHG EETKYIETHT HHFANFTTSQ RQGLTFESNK VTVTISSEDG EFLVDNIALV
     EAPLPTDDQN SEGNTASSTN SDTSMNNNQ