ID   CR5AC_BACTU             Reviewed;        1220 AA.
AC   P56955;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Pesticidal crystal protein Cry5Ac;
DE   AltName: Full=135 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryVA(c);
GN   Name=cry5Ac; Synonyms=cryVA(c);
OS   Bacillus thuringiensis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1428;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NRRL B-18765 / PS86Q3;
RA   Payne J.M., Kennedy M.K., Randall J.B., Meier H., Uick H.J., Foncerrada L.,
RA   Schnepf H.E., Schwab G.E., Fu J.M.;
RT   "Bacillus thuringiensis toxins active against hymenopteran pests.";
RL   Patent number US5596071, 21-JAN-1997.
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of hymenopteran species.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; I34543; -; NOT_ANNOTATED_CDS; Unassigned_DNA.
DR   AlphaFoldDB; P56955; -.
DR   SMR; P56955; -.
DR   TCDB; 1.C.2.3.3; the channel-forming Delta-endotoxin insecticidal crystal protein (icp) family.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Sporulation; Toxin; Virulence.
FT   CHAIN           1..1220
FT                   /note="Pesticidal crystal protein Cry5Ac"
FT                   /id="PRO_0000174068"
FT   REGION          1194..1220
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1198..1220
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1220 AA;  135403 MW;  CE6A314FF687F79C CRC64;
     MAILNELYPS VPYNVLAYTP PSFLPDAGTQ ATPADLTAYE QLLKNLEKGI NAGTYSKAIA
     DVLKGIFIDD TINYQTYVNI GLSLITLAVP EIGIFTPFIG LFFAALNKHD APPPPNAKDI
     FEAMKPAIQE MIDRTLTADE QTFLNGEISG LQNLAARYQS TMDDIQSHGG FNKVDSGLIK
     KFTDEVLSLN SFYTDRLPVF ITDNTADRTL LGLPYYAILA SMHLMLLRDI ITKGPTWDSK
     INFTPDAIDS FKTDIKNNIK LYSKTIYDVF QKGLASYGTP SDLESFAKKK KYIEIMTTHC
     LDFARLFPTF DPDLYPTGSG DISLQKTRRI LSPFIPIRTA DGLTLNNTSI DTSNWPNYEN
     GNGAFPNPKE RILKQFKLYP SWRAGQYGGL LQPYLWAIEV QDSVETRLYG QLPAVDPQAG
     PNYVSIDSSN PIIQINMDTW KTPPQGASGW NTNLMRGSVS GLSFLQRDGT RLSAGMGGGF
     ADTIYSLPAT HYLSYLYGTP YQTSDNYSGH VGALVGVSTP QEATLPNIIG QPDEQGNVST
     MGFPFEKASY GGTVVKEWLN GANAMKLSPG QSIGIPITNV TKHNYQVRCR YASNSDNPVF
     FNVDTGGANP IFQQINFAST VDSNMGVKEE NGVYVVKSIK TVEIPAGSFY VHVTNQGSSD
     LFLDRIEFVP KIQFQFCDNN NLHCDCNNPV DTDCTFCCVC TSLTDCDCNN PRGIDCTLCC
     QVENQLPSFV TLTDLRNITS QVNGLFAPGT QNRLAQNISD HDIEEVVLKV DALSDEIFGT
     NKKALRKLVN QAKRLSRARN LLIGGSFENW DAWYKGRNVV TVSDHELFKS DHVLLPPPGL
     SPSYIFQKVE ESKLKANTRY TVSGFIAHAT DLEIVVSRYG QEIKKVVQVP YGEAFPLTSS
     GPVCCIPHST SNGTLGNPHF FSYSIDVGAL DVDTNPGIEF GLRIVNPTGM ARVSNLEIRE
     DRPLAANEIR QVQRVARNWR TEYEKERAEV TSLIQPVINR INGLYENENW NGSIRSDISY
     QNIDAIVLPT LPTLRHWFMS DRFSEQGDIM AKFQGALNRA YAQLEQSTLL HNGHFTKDAA
     NWTIEGDAHQ ITLEDGRRVL RLPDWSSSVS QMIEIENFNP DKEYNLVFHG QGEGTVTLEH
     GEETKYIETH THHFANFTTS QRQGLTFESN KVTVTISSED GEFLVDNIAL VEAPLPTDDQ
     NSEGNTAFST NSDTSMNNNQ