CR5BA_BACTU
ID CR5BA_BACTU Reviewed; 1245 AA.
AC Q45712;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 81.
DE RecName: Full=Pesticidal crystal protein Cry5Ba;
DE AltName: Full=140 kDa crystal protein;
DE AltName: Full=Crystaline entomocidal protoxin;
DE AltName: Full=Insecticidal delta-endotoxin CryVB(a);
GN Name=cry5Ba; Synonyms=cryVB(a);
OS Bacillus thuringiensis.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=1428;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-15.
RC STRAIN=NRRL B-18765 / PS86Q3;
RA Payne J.M., Kennedy M.K., Randall J.B., Meier H., Uick H.J., Foncerrada L.,
RA Schnepf H.E., Schwab G.E., Fu J.M.;
RT "Bacillus thuringiensis toxins active against hymenopteran pests.";
RL Patent number US5596071, 21-JAN-1997.
CC -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC epithelial cells of hymenopteran species.
CC -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC and is accumulated both as an inclusion and as part of the spore coat.
CC -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC terminus.
CC -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR EMBL; U19725; AAA68598.1; -; Genomic_DNA.
DR PIR; T18211; T18211.
DR PDB; 4D8M; X-ray; 2.30 A; A=112-698.
DR PDBsum; 4D8M; -.
DR AlphaFoldDB; Q45712; -.
DR SMR; Q45712; -.
DR GO; GO:0090729; F:toxin activity; IDA:UniProtKB.
DR GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 1.20.190.10; -; 1.
DR InterPro; IPR041587; Cry_V.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR005638; Pest_crys_C.
DR InterPro; IPR005639; Pest_crys_N.
DR InterPro; IPR036716; Pest_crys_N_sf.
DR Pfam; PF17997; Cry1Ac_D5; 1.
DR Pfam; PF03944; Endotoxin_C; 1.
DR Pfam; PF03945; Endotoxin_N; 1.
DR SUPFAM; SSF49785; SSF49785; 1.
DR SUPFAM; SSF56849; SSF56849; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Sporulation; Toxin; Virulence.
FT CHAIN 1..1245
FT /note="Pesticidal crystal protein Cry5Ba"
FT /id="PRO_0000174069"
FT REGION 1219..1245
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1223..1245
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 115..161
FT /evidence="ECO:0007829|PDB:4D8M"
FT TURN 175..177
FT /evidence="ECO:0007829|PDB:4D8M"
FT HELIX 187..207
FT /evidence="ECO:0007829|PDB:4D8M"
FT HELIX 208..211
FT /evidence="ECO:0007829|PDB:4D8M"
FT HELIX 222..256
FT /evidence="ECO:0007829|PDB:4D8M"
FT HELIX 262..290
FT /evidence="ECO:0007829|PDB:4D8M"
FT HELIX 299..315
FT /evidence="ECO:0007829|PDB:4D8M"
FT HELIX 317..320
FT /evidence="ECO:0007829|PDB:4D8M"
FT HELIX 321..326
FT /evidence="ECO:0007829|PDB:4D8M"
FT TURN 328..330
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 335..337
FT /evidence="ECO:0007829|PDB:4D8M"
FT HELIX 363..365
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 367..371
FT /evidence="ECO:0007829|PDB:4D8M"
FT HELIX 373..375
FT /evidence="ECO:0007829|PDB:4D8M"
FT HELIX 388..390
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 398..406
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 415..423
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 428..435
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 442..452
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 454..464
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 466..468
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 470..481
FT /evidence="ECO:0007829|PDB:4D8M"
FT HELIX 489..495
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 510..520
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 531..538
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 543..547
FT /evidence="ECO:0007829|PDB:4D8M"
FT TURN 552..554
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 561..565
FT /evidence="ECO:0007829|PDB:4D8M"
FT HELIX 571..573
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 580..583
FT /evidence="ECO:0007829|PDB:4D8M"
FT HELIX 584..586
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 588..593
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 598..605
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 609..621
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 623..629
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 632..634
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 636..642
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 649..653
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 655..669
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 671..680
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 682..684
FT /evidence="ECO:0007829|PDB:4D8M"
FT STRAND 686..695
FT /evidence="ECO:0007829|PDB:4D8M"
SQ SEQUENCE 1245 AA; 139776 MW; C7CB93F96650171F CRC64;
MATINELYPV PYNVLAHPIK EVDDPYSWSN LLKGIQEGWE EWGKTGQKKL FEDHLTIAWN
LYKTGKLDYF ALTKASISLI GFIPGAEAAV PFINMFVDFV WPKLFGANTE GKDQQLFNAI
MDAVNKMVDN KFLSYNLSTL NKTIEGLQGN LGLFQNAIQV AICQGSTPER VNFDQNCTPC
NPNQPCKDDL DRVASRFDTA NSQFTQHLPE FKNPWSDENS TQEFKRTSVE LTLPMYTTVA
TLHLLLYEGY IEFMTKWNFH NEQYLNNLKV ELQQLIHSYS ETVRTSFLQF LPTLNNRSKS
SVNAYNRYVR NMTVNCLDIA ATWPTFDTHN YHQGGKLDLT RIILSDTAGP IEEYTTGDKT
SGPEHSNITP NNILDTPSPT YQHSFVSVDS IVYSRKELQQ LDIATYSTNN SNNCHPYGLR
LSYTDGSRYD YGDNQPDFTT SNNNYCHNSY TAPITLVNAR HLYNAKGSLQ NVESLVVSTV
NGGSGSCICD AWINYLRPPQ TSKNESRPDQ KINVLYPITE TVNKGTGGNL GVISAYVPME
LVPENVIGDV NADTKLPLTQ LKGFPFEKYG SEYNNRGISL VREWINGNNA VKLSNSQSVG
IQITNQTKQK YEIRCRYASK GDNNVYFNVD LSENPFRNSI SFGSTESSVV GVQGENGKYI
LKSITTVEIP AGSFYVHITN QGSSDLFLDR IEFVPKIQFQ FCDNNNLHCD CNNPVDTDCT
FCCVCTSLTD CDCNNPRGLD CTLCCQVENQ LPSFVTLTDL QNITTQVNAL VASSEHDTLA
TDVSDYEIEE VVLKVDALSG EVFGKEKKAL RKLVNHTKRL SKARNLLIGG NFDNLDAWYR
GRNVVNVSDH ELFKSDHVLL PPPTLYSSYM FQKVEESKLK ANTRYTVSGF IAHAEDLEIV
VSRYGQEVKK VVQVPYGEAF PLTSRGAICC PPRSTSNGKP ADPHFFSYSI DVGTLDVEAN
PGIELGLRIV ERTGMARVSN LEIREDRPLK KNELRNVQRA ARNWRTAYDQ ERAEVTALIQ
PVLNQINALY ENEDWNGAIR SGVSYHDLEA IVLPTLPKLN HWFMSDMLGE QGSILAQFQE
ALDRAYTQLE ESTILHNGHF TTDAANWTIE GDAHHAILED GRRVLRLPDW SSSVSQTIEI
ENFDPDKEYQ LVFHAQGEGT VSLQHGEEGE YVETHPHKSA NFTTSHRQGV TFETNKVTVE
ITSEDGEFLV DHIALVEAPL PTDDQSSDGN TTSNTNSNTS MNNNQ
