2SS4_ARATH
ID 2SS4_ARATH Reviewed; 166 AA.
AC P15460;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=2S seed storage protein 4;
DE AltName: Full=2S albumin storage protein;
DE AltName: Full=NWMU2-2S albumin 4;
DE Contains:
DE RecName: Full=2S seed storage protein 4 small subunit;
DE Contains:
DE RecName: Full=2S seed storage protein 4 large subunit;
DE Flags: Precursor;
GN Name=AT2S4; OrderedLocusNames=At4g27170; ORFNames=T24A18.120;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. C24;
RX PubMed=16666238; DOI=10.1104/pp.87.4.859;
RA Krebbers E., Herdies L., de Clercq A., Seurinck J., Leemans J.,
RA van Damme J., Segura M., Gheysen G., van Montagu M., Vandekerckhove J.;
RT "Determination of the processing sites of an Arabidopsis 2S albumin and
RT characterization of the complete gene family.";
RL Plant Physiol. 87:859-866(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. C24;
RX PubMed=8012403; DOI=10.1046/j.1365-313x.1994.05040493.x;
RA da Silva Conceicao A., Krebbers E.;
RT "A cotyledon regulatory region is responsible for the different spatial
RT expression patterns of Arabidopsis 2S albumin genes.";
RL Plant J. 5:493-505(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia; TISSUE=Green siliques;
RX PubMed=8281187; DOI=10.1046/j.1365-313x.1993.04061051.x;
RA Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M.,
RA Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D.,
RA Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M.,
RA Cooke R., Grellet F., Delseny M., Parmentier Y., de Marcillac G., Gigot C.,
RA Fleck J., Philipps G., Axelos M., Bardet C., Tremousaygue D., Lescure B.;
RT "An inventory of 1152 expressed sequence tags obtained by partial
RT sequencing of cDNAs from Arabidopsis thaliana.";
RL Plant J. 4:1051-1061(1993).
CC -!- FUNCTION: This is a 2S seed storage protein.
CC -!- SUBUNIT: The mature protein consists of a small and a large chain
CC linked by disulfide bonds.
CC -!- SIMILARITY: Belongs to the 2S seed storage albumins family.
CC {ECO:0000305}.
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DR EMBL; AH001334; AAA32746.1; -; Genomic_DNA.
DR EMBL; Z24744; CAA80869.1; -; Genomic_DNA.
DR EMBL; AL035680; CAB38847.1; -; Genomic_DNA.
DR EMBL; AL161566; CAB79572.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85309.1; -; Genomic_DNA.
DR EMBL; AF446894; AAL38627.1; -; mRNA.
DR EMBL; AY052682; AAK96586.1; -; mRNA.
DR EMBL; Z17597; CAA79009.1; -; mRNA.
DR EMBL; Z17601; CAA79011.1; -; mRNA.
DR PIR; JA0164; NWMU4.
DR RefSeq; NP_194447.1; NM_118851.1.
DR AlphaFoldDB; P15460; -.
DR SMR; P15460; -.
DR BioGRID; 14112; 1.
DR STRING; 3702.AT4G27170.1; -.
DR PaxDb; P15460; -.
DR PRIDE; P15460; -.
DR ProteomicsDB; 245162; -.
DR EnsemblPlants; AT4G27170.1; AT4G27170.1; AT4G27170.
DR GeneID; 828825; -.
DR Gramene; AT4G27170.1; AT4G27170.1; AT4G27170.
DR KEGG; ath:AT4G27170; -.
DR Araport; AT4G27170; -.
DR TAIR; locus:2136496; AT4G27170.
DR eggNOG; ENOG502S7EV; Eukaryota.
DR HOGENOM; CLU_131213_1_0_1; -.
DR InParanoid; P15460; -.
DR OMA; RCEIRTR; -.
DR OrthoDB; 1466684at2759; -.
DR PhylomeDB; P15460; -.
DR PRO; PR:P15460; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; P15460; baseline and differential.
DR Genevisible; P15460; AT.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR CDD; cd00261; AAI_SS; 1.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR044723; AAI_SS_dom.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR InterPro; IPR000617; Napin/2SS/CON.
DR PANTHER; PTHR35496; PTHR35496; 1.
DR Pfam; PF00234; Tryp_alpha_amyl; 1.
DR PRINTS; PR00496; NAPIN.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Reference proteome; Seed storage protein; Signal;
KW Storage protein.
FT SIGNAL 1..21
FT PROPEP 22..37
FT /id="PRO_0000032101"
FT CHAIN 38..72
FT /note="2S seed storage protein 4 small subunit"
FT /evidence="ECO:0000250"
FT /id="PRO_0000032102"
FT PROPEP 73..88
FT /id="PRO_0000032103"
FT CHAIN 89..166
FT /note="2S seed storage protein 4 large subunit"
FT /evidence="ECO:0000250"
FT /id="PRO_0000032104"
SQ SEQUENCE 166 AA; 19169 MW; 6E136F2D11F5039E CRC64;
MANKLFLVCA ALALCFILTN ASVYRTVVEF DEDDASNPIG PIQKCQKEFQ QDQHLRACQR
WMRKQMWQGR GGGPSLDDEF DMEDDIENPQ RRQLLQKCCS ELRQEEPVCV CPTLRQAAKA
VRFQGQQHQP EQVRKIYQAA KYLPNICKIQ QVGVCPFQIP SIPSYY
