ID   CR7AA_BACTU             Reviewed;        1138 AA.
AC   Q03749;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Pesticidal crystal protein Cry7Aa;
DE   AltName: Full=129 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryVIIA(a);
GN   Name=cry7Aa; Synonyms=cryIIIc, cryVIIA(a);
OS   Bacillus thuringiensis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1428;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1514800; DOI=10.1128/aem.58.8.2536-2542.1992;
RA   Lambert B., Hofte H., Annys K., Jansens S., Soetaert P., Peferoen M.;
RT   "Novel Bacillus thuringiensis insecticidal crystal protein with a silent
RT   activity against coleopteran larvae.";
RL   Appl. Environ. Microbiol. 58:2536-2542(1992).
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of Coleoptera. This protein is not toxic in its
CC       natural form. It is highly toxic to Colorado potato beetle larvae after
CC       an in vitro solubilization and trypsin activation step.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; M64478; AAA22351.1; -; Genomic_DNA.
DR   PIR; A48944; A48944.
DR   AlphaFoldDB; Q03749; -.
DR   SMR; Q03749; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR038979; Pest_crys.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   PANTHER; PTHR37003; PTHR37003; 1.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 2.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Sporulation; Toxin; Virulence.
FT   CHAIN           1..1138
FT                   /note="Pesticidal crystal protein Cry7Aa"
FT                   /id="PRO_0000174072"
SQ   SEQUENCE   1138 AA;  129392 MW;  69D8676D4F6A1FAC CRC64;
     MNLNNLDGYE DSNRTLNNSL NYPTQKALSP SLKNMNYQDF LSITEREQPE ALASGNTAIN
     TVVSVTGATL SALGVPGASF ITNFYLKIAG LLWPENGKIW DEFMTEVEAL IDQKIEEYVR
     NKAIAELDGL GSALDKYQKA LADWLGKQDD PEAILSVATE FRIIDSLFEF SMPSFKVTGY
     EIPLLTVYAQ AANLHLALLR DSTLYGDKWG FTQNNIEENY NRQKKRISEY SDHCTKWYNS
     GLSRLNGSTY EQWINYNRFR REMILMALDL VAVFPFHDPR RYSMETSTQL TREVYTDPVS
     LSISNPDIGP SFSQMENTAI RTPHLVDYLD ELYIYTSKYK AFSHEIQPDL FYWSAHKVSF
     KKSEQSNLYT TGIYGKTSGY ISSGAYSFHG NDIYRTLAAP SVVVYPYTQN YGVEQVEFYG
     VKGHVHYRGD NKYDLTYDSI DQLPPDGEPI HEKYTHRLCH ATAIFKSTPD YDNATIPIFS
     WTHRSAEYYN RIYPNKITKI PAVKMYKLDD PSTVVKGPGF TGGDLVKRGS TGYIGDIKAT
     VNSPLSQKYR VRVRYATNVS GQFNVYINDK ITLQTKFQNT VETIGEGKDL TYGSFGYIEY
     STTIQFPDEH PKITLHLSDL SNNSSFYVDS IEFIPVDVNY AEKEKLEKAQ KAVNTLFTEG
     RNALQKDVTD YKVDQVSILV DCISGDLYPN EKRELQNLVK YAKRLSYSRN LLLDPTFDSI
     NSSEENGWYG SNGIVIGNGD FVFKGNYLIF SGTNDTQYPT YLYQKIDESK LKEYTRYKLK
     GFIESSQDLE AYVIRYDAKH RTLDVSDNLL PDILPENTCG EPNRCAAQQY LDENPSPECS
     SMQDGILSDS HSFSLNIDTG SINHNENLGI WVLFKISTLE GYAKFGNLEV IEDGPVIGEA
     LARVKRQETK WRNKLAQLTT ETQAIYTRAK QALDNLFANA QDSHLKRDVT FAEIAAARKI
     VQSIREAYMS WLSVVPGVNH PIFTELSGRV QRAFQLYDVR NVVRNGRFLN GLSDWIVTSD
     VKVQEENGNN VLVLNNWDAQ VLQNVKLYQD RGYILHVTAR KIGIGEGYIT ITDEEGHTDQ
     LRFTACEEID ASNAFISGYI TKELEFFPDT EKVHIEIGET EGIFLVESIE LFLMEELC