ID   CR8AA_BACUK             Reviewed;        1157 AA.
AC   Q45704;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Pesticidal crystal protein Cry8Aa;
DE   AltName: Full=131 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryVIIIA(a);
GN   Name=cry8Aa; Synonyms=cryVIIIA(a);
OS   Bacillus thuringiensis serovar kumamotoensis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=132267;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NRRL B-18746 / PS50C;
RA   Payne J.M., Sick A.J., Foncerrada L.;
RT   "Novel coleopteran-active Bacillus thuringiensis isolate and a novel gene
RT   encoding a coleopteran-active toxin.";
RL   Patent number EP0498537, 12-AUG-1992.
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of Coleoptera.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; U04364; AAA21117.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q45704; -.
DR   SMR; Q45704; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR038979; Pest_crys.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   PANTHER; PTHR37003; PTHR37003; 1.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 2.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Sporulation; Toxin; Virulence.
FT   CHAIN           1..1157
FT                   /note="Pesticidal crystal protein Cry8Aa"
FT                   /id="PRO_0000174075"
FT   REGION          1..29
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1157 AA;  131010 MW;  687B5C49DE93683B CRC64;
     MSPNNQNEYE IIDATPSTSV SSDSNRYPFA NEPTDALQNM NYKDYLKMSG GENPELFGNP
     ETFISSSTIQ TGIGIVGRIL GALGVPFASQ IASFYSFIVG QLWPSKSVDI WGEIMERVEE
     LVDQKIEKYV KDKALAELKG LGNALDVYQQ SLEDWLENRN DARTRSVVSN QFIALDLNFV
     SSIPSFAVSG HEVLLLAVYA QAVNLHLLLL RDASIFGEEW GFTPGEISRF YNRQVQLTAE
     YSDYCVKWYK IGLDKLKGTT SKSWLNYHQF RREMTLLVLD LVALFPNYDT HMYPIETTAQ
     LTRDVYTDPI AFNIVTSTGF CNPWSTHSGI LFYEVENNVI RPPHLFDILS SVEINTSRGG
     ITLNNDAYIN YWSGHTLKYR RTADSTVTYT ANYGRITSEK NSFALEDRDI FEINSTVANL
     ANYYQKAYGV PGSWFHMVKR GTSSTTAYLY SKTHTALQGC TQVYESSDEI PLDRTVPVAE
     SYSHRLSHIT SHSFSKNGSA YYGSFPVFVW THTSADLNNT IYSDKITQIP AVKGDMLYLG
     GSVVQGPGFT GGDILKRTNP SILGTFAVTV NGSLSQRYRV RIRYASTTDF EFTLYLGDTI
     EKNRFNKTMD NGASLTYETF KFASFITDFQ FRETQDKILL SMGDFSSGQE VYIDRIEFIP
     VDETYEAEQD LEAAKKAVNA LFTNTKDGLR PGVTDYEVNQ AANLVECLSD DLYPNEKRLL
     FDAVREAKRL SGARNLLQDP DFQEINGENG WAASTGIEIV EGDAVFKGRY LRLPGAREID
     TETYPTYLYQ KVEEGVLKPY TRYRLRGFVG SSQGLEIYTI RHQTNRIVKN VPDDLLPDVS
     PVNSDGSINR CSEQKYVNSR LEGENRSGDA HEFSLPIDIG ELDYNENAGI WVGFKITDPE
     GYATLGNLEL VEEGPLSGDA LERLQREEQQ WKIQMTRRRE ETDRRYMASK QAVDRLYADY
     QDQQLNPDVE ITDLTAAQDL IQSIPYVYNE MFPEIPGMNY TKFTELTDRL QQAWNLYDQR
     NAIPNGDFRN GLSNWNATPG VEVQQINHTS VLVIPNWDEQ VSQQFTVQPN QRYVLRVTAR
     KEGVGNGYVS IRDGGNQSET LTFSASDYDT NGVYNDQTGY ITKTVTFIPY TDQMWIEISE
     TEGTFYIESV ELIVDVE