ID   CR8BA_BACUK             Reviewed;        1169 AA.
AC   Q45705;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Pesticidal crystal protein Cry8Ba;
DE   AltName: Full=134 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryVIIIB(a);
GN   Name=cry8Ba; Synonyms=50C(b), cryVIIIB(a);
OS   Bacillus thuringiensis serovar kumamotoensis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=132267;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NRRL B-18746 / PS50C;
RA   Michaels T.E., Foncerrada L., Narva K.E.;
RT   "Process for controlling scarab pests with Bacillus thuringiensis
RT   isolates.";
RL   Patent number WO9315206, 05-AUG-1993.
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of insects. Active on various scarabaeid beetles.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; U04365; AAA21118.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q45705; -.
DR   SMR; Q45705; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR038979; Pest_crys.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   PANTHER; PTHR37003; PTHR37003; 1.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 2.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Sporulation; Toxin; Virulence.
FT   CHAIN           1..1169
FT                   /note="Pesticidal crystal protein Cry8Ba"
FT                   /id="PRO_0000174076"
FT   REGION          1..26
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1169 AA;  133544 MW;  22EEFCF5BD699909 CRC64;
     MSPNNQNEYE IIDATPSTSV SNDSNRYPFA NEPTNALQNM DYKDYLKMSA GNVSEYPGSP
     EVFLSEQDAV KAAIDIVGKL LTGLGVPFVG PIVSLYTQLI DILWPSKQKS QWEIFMEQVE
     ELINQKIAEY ARNKALSELE GLGNNYQLYL TALEEWKENP NGSRALRDVR NRFEILDSLF
     TQYMPSFRVT NFEVPFLTVY TMAANLHLLL LRDASIFGEE WGLSTSTINN YYNRQMKLTA
     EYSDHCVKWY ETGLAKLKGS SAKQWIDYNQ FRREMTLTVL DVVALFSNYD TRTYPLATTA
     QLTREVYTDP LGAVDVPNIG SWYDKAPSFS EIEKAAIRPP HVFDYITGLT VYTKKRSFTS
     DRYMRYWAGH QISYKHIGTS STFTQMYGTN QNLQSTSNFD FTNYDIYKTL SNGAVLLDIV
     YPGYTYTFFG MPETEFFMVN QLNNTRKTLT YKPASKDIID RTRDSELELP PETSGQPNYE
     SYSHRLGHIT FIYSSSTSTY VPVFSWTHRS ADLTNTVKSG EITQIPGGKS STIGRNTYII
     KGRGYTGGDL VALTDRIGSC EFQMIFPESQ RFRIRIRYAS NETSYISLYG LNQSGTLKFN
     QTYSNKNEND LTYNDFKYIE YPRVISVNAS SNIQRLSIGI QTNTNLFILD RIEFIPVDET
     YEAETDLEAA KKAVNALFTN TKDGLQPGVT DYEVNQAANL VECLSDDLYP NEKRLLFDAV
     REAKRLSEAR NLLQDPDFQE INGENGWTAS TGIEVIEGDA VFKGRYLRLP GAREIDTETY
     PTYLYQKVEE GVLKPYTRYR LRGFVGSSQG LEIYTIRHQT NRIVKNVPDD LLPDVPPVNN
     DGRINRCSEQ KYVNSRLEVE NRSGEAHEFS IPIDTGELDY NENAGIWVGF KITDPEGYAT
     LGNLELVEEG PLSGDALERL QKEEQQWKIQ MTRRREETDR RYMASKQAVD RLYADYQDQQ
     LNPNVEITDL TAAQDLIQSI PYVYNEMFPE IPGMNYTKFT ELTDRLQQAW GLYDQRNAIP
     NGDYRNELSN WNTTSGVNVQ QINHTSVLVI PNWNEQVSQK FTVQPNQRYV LRVTARKEGV
     GNGYVSIRDG GNQSETLTFS ASDYDTNGMY DTQASNTNGY NTNSVYMIKP AISRKTVDIS
     SVYNQMWIEI SETEGTFYIE SVELIVDVE