ID   CR8CA_BACTP             Reviewed;        1160 AA.
AC   Q45706;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Pesticidal crystal protein Cry8Ca;
DE   AltName: Full=130 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryVIIIC(a);
GN   Name=cry8Ca; Synonyms=cryIII, cryVIIIC(a);
OS   Bacillus thuringiensis subsp. japonensis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=128936;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Buibui;
RX   PubMed=7764305; DOI=10.1007/bf01575980;
RA   Sato R., Takeuchi K., Ogiwara K., Minami M., Kaji Y., Suzuki N., Hori H.,
RA   Asano S., Ohba M., Iwahana H.;
RT   "Cloning, heterologous expression, and localization of a novel crystal
RT   protein gene from Bacillus thuringiensis serovar japonensis strain buibui
RT   toxic to scarabaeid insects.";
RL   Curr. Microbiol. 28:15-19(1994).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-14 AND 56-64, AND CHARACTERIZATION.
RX   PubMed=8200856; DOI=10.1111/j.1365-2672.1994.tb01633.x;
RA   Hori H., Suzuki N., Ogiwara K., Himejima M., Indrasith L.S., Minami M.,
RA   Asano S., Sato R., Ohba M., Iwahana H.;
RT   "Characterization of larvicidal toxin protein from Bacillus thuringiensis
RT   serovar japonensis strain Buibui specific for scarabaeid beetles.";
RL   J. Appl. Bacteriol. 76:307-313(1994).
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of insects. Active on various scarabaeid beetles such
CC       as Anomala cuprea, A.rufocuprea and Popillia japonica.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U04366; AAA21119.1; -; Genomic_DNA.
DR   PIR; I40589; I40589.
DR   AlphaFoldDB; Q45706; -.
DR   SMR; Q45706; -.
DR   PRIDE; Q45706; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR038979; Pest_crys.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   PANTHER; PTHR37003; PTHR37003; 1.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 2.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Sporulation; Toxin; Virulence.
FT   CHAIN           1..1160
FT                   /note="Pesticidal crystal protein Cry8Ca"
FT                   /id="PRO_0000174077"
SQ   SEQUENCE   1160 AA;  130426 MW;  C16C3D912EEB8751 CRC64;
     MSPNNQNEYE IIDALSPTSV SDNSIRYPLA NDQTNTLQNM NYKDYLKMTE STNAELSRNP
     GTFISAQDAV GTGIDIVSTI ISGLGIPVLG EVFSILGSLI GLLWPSNNEN VWQIFMNRVE
     ELIDQKILDS VRSRAIADLA NSRIAVEYYQ NALEDWRKNP HSTRSAALVK ERFGNAEAIL
     RTNMGSFSQT NYETPLLPTY AQAASLHLLV MRDVQIYGKE WGYPQNDIDL FYKEQVSYTA
     RYSDHCVQWY NAGLNKLRGT GAKQWVDYNR FRREMNVMVL DLVALFPNYD ARIYPLETNA
     ELTREIFTDP VGSYVTGQSS TLISWYDMIP AALPSFSTLE NLLRKPDFFT LLQEIRMYTS
     FRQNGTIEYY NYWGGQRLTL SYIYGSSFNK YSGVLAGAED IIPVGQNDIY RVVWTYIGRY
     TNSLLGVNPV TFYFSNNTQK TYSKPKQFAG GIKTIDSGEE LTYENYQSYS HRVSYITSFE
     IKSTGGTVLG VVPIFGWTHS SASRNNFIYA TKISQIPINK ASRTSGGAVW NFQEGLYNGG
     PVMKLSGSGS QVINLRVATD AKGASQRYRI RIRYASDRAG KFTISSRSPE NPATYSASIA
     YTNTMSTNAS LTYSTFAYAE SGPINLGISG SSRTFDISIT KEAGAANLYI DRIEFIPVNT
     LFEAEEDLDV AKKAVNGLFT NEKDALQTSV TDYQVNQAAN LIECLSDELY PNEKRMLWDA
     VKEAKRLVQA RNLLQDTGFN RINGENGWTG STGIEVVEGD VLFKDRSLRL TSAREIDTET
     YPTYLYQQID ESLLKPYTRY KLKGFIGSSQ DLEIKLIRHR ANQIVKNVPD NLLPDVRPVN
     SCGGVDRCSE QQYVDANLAL ENNGENGNMS SDSHAFSFHI DTGEIDLNEN TGIWIVFKIP
     TTNGNATLGN LEFVEEGPLS GETLEWAQQQ EQQWQDKMAR KRAASEKTYY AAKQAIDRLF
     ADYQDQKLNS GVEMSDLLAA QNLVQSIPYV YNDALPEIPG MNYTSFTELT NRLQQAWNLY
     DLQNAIPNGD FRNGLSNWNA TSDVNVQQLS DTSVLVIPNW NSQVSQQFTV QPNYRYVLRV
     TARKEGVGDG YVIIRDGANQ TETLTFNICD DDTGVLSTDQ TSYITKTVEF TPSTEQVWID
     MSETEGVFNI ESVELVLEEE