ID   CR9AA_BACTG             Reviewed;        1156 AA.
AC   Q99031; Q03747;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Pesticidal crystal protein Cry9Aa;
DE   AltName: Full=130 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryIXA(a);
DE   Flags: Precursor;
GN   Name=cry9Aa; Synonyms=cryIG, cryIVA(a);
OS   Bacillus thuringiensis subsp. galleriae.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=29338;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 24-34.
RC   STRAIN=11-67;
RX   PubMed=1660003; DOI=10.1016/0014-5793(91)81144-w;
RA   Smulevitch S.V., Osterman A.L., Shevelev A.B., Kaluger S.V., Karasin A.I.,
RA   Kadyrov R.M., Zagnitko O.P., Chestukhina G.G., Stepanov V.M.;
RT   "Nucleotide sequence of a novel delta-endotoxin gene cryIg of Bacillus
RT   thuringiensis ssp. galleriae.";
RL   FEBS Lett. 293:25-28(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-1151.
RC   STRAIN=DSIR517;
RX   PubMed=1556556; DOI=10.1099/00221287-138-1-55;
RA   Gleave A.P., Hedges R.J., Broadwell A.H.;
RT   "Identification of an insecticidal crystal protein from Bacillus
RT   thuringiensis DSIR517 with significant sequence differences from previously
RT   described toxins.";
RL   J. Gen. Microbiol. 138:55-62(1992).
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of insects. This protein is toxic to Galleria
CC       mellonella.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; X58120; CAA41122.1; -; Genomic_DNA.
DR   EMBL; X58534; CAA41425.1; -; Genomic_DNA.
DR   PIR; S19306; S19306.
DR   AlphaFoldDB; Q99031; -.
DR   SMR; Q99031; -.
DR   PRIDE; Q99031; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR038979; Pest_crys.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   PANTHER; PTHR37003; PTHR37003; 1.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 2.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Sporulation; Toxin; Virulence.
FT   PROPEP          1..23
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000269|PubMed:1660003"
FT                   /id="PRO_0000006347"
FT   CHAIN           24..1156
FT                   /note="Pesticidal crystal protein Cry9Aa"
FT                   /id="PRO_0000006348"
SQ   SEQUENCE   1156 AA;  129727 MW;  785F65B1B1165FF2 CRC64;
     MNQNKHGIIG ASNCGCASDD VAKYPLANNP YSSALNLNSC QNSSILNWIN IIGDAAKEAV
     SIGTTIVSLI TAPSLTGLIS IVYDLIGKVL GGSSGQSISD LSICDLLSII DLRVSQSVLN
     DGIADFNGSV LLYRNYLEAL DSWNKNPNSA SAEELRTRFR IADSEFDRIL TRGSLTNGGS
     LARQNAQILL LPSFASAAFF HLLLLRDATR YGTNWGLYNA TPFINYQSKL VELIELYTDY
     CVHWYNRGFN ELRQRGTSAT AWLEFHRYRR EMTLMVLDIV ASFSSLDITN YPIETDFQLS
     RVIYTDPIGF VHRSSLRGES WFSFVNRANF SDLENAIPNP RPSWFLNNMI ISTGSLTLPV
     SPSTDRARVW YGSRDRISPA NSQFITELIS GQHTTATQTI LGRNIFRVDS QACNLNDTTY
     GVNRAVFYHD ASEGSQRSVY EGYIRTTGID NPRVQNINTY LPGENSDIPT PEDYTHILST
     TINLTGGLRQ VASNRRSSLV MYGWTHKSLA RNNTINPDRI TQIPLTKVDT RGTGVSYVND
     PGFIGGALLQ RTDHGSLGVL RVQFPLHLRQ QYRIRVRYAS TTNIRLSVNG SFGTISQNLP
     STMRLGEDLR YGSFAIREFN TSIRPTASPD QIRLTIEPSF IRQEVYVDRI EFIPVNPTRE
     AKEDLEAAKK AVASLFTRTR DGLQVNVKDY QVDQAANLVS CLSDEQYGYD KKMLLEAVRA
     AKRLSRERNL LQDPDFNTIN STEENGWKAS NGVTISEGGP FYKGRAIQLA SARENYPTYI
     YQKVDASELK PYTRYRLDGF VKSSQDLEID LIHHHKVHLV KNVPDNLVSD TYPDDSCSGI
     NRCQEQQMVN AQLETEHHHP MDCCEAAQTH EFSSYIDTGD LNSSVDQGIW AIFKVRTTDG
     YATLGNLELV EVGPLSGESL EREQRDNTKW SAELGRKRAE TDRVYQDAKQ SINHLFVDYQ
     DQQLNPEIGM ADIMDAQNLV ASISDVYSDA VLQIPGINYE IYTELSNRLQ QASYLYTSRN
     AVQNGDFNNG LDSWNATAGA SVQQDGNTHF LVLSHWDAQV SQQFRVQPNC KYVLRVTAEK
     VGGGDGYVTI RDDAHHTETL TFNACDYDIN GTYVTDNTYL TKEVVFHPET QHMWVEVNET
     EGAFHIDSIE FVETEK