ID   CR9DA_BACTP             Reviewed;        1169 AA.
AC   O06014;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Pesticidal crystal protein Cry9Da;
DE   AltName: Full=132 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryIXD(a);
GN   Name=cry9Da; Synonyms=cryIXD(a);
OS   Bacillus thuringiensis subsp. japonensis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=128936;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=N141;
RA   Asano S.;
RL   Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of insects.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; D85560; BAA19948.1; -; Genomic_DNA.
DR   AlphaFoldDB; O06014; -.
DR   SMR; O06014; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR038979; Pest_crys.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   PANTHER; PTHR37003; PTHR37003; 1.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 2.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Sporulation; Toxin; Virulence.
FT   CHAIN           1..1169
FT                   /note="Pesticidal crystal protein Cry9Da"
FT                   /id="PRO_0000174079"
SQ   SEQUENCE   1169 AA;  132229 MW;  659AB257229DE5E9 CRC64;
     MNRNNQNEYE VIDAPHCGCP ADDVVKYPLT DDPNAGLQNM NYKEYLQTYG GDYTDPLINP
     NLSVSGKDVI QVGINIVGRL LSFFGFPFSS QWVTVYTYLL NSLWPDDENS VWDAFMERVE
     ELIDQKISEA VKGRALDDLT GLQYNYNLYV EALDEWLNRP NGARASLVSQ RFNILDSLFT
     QFMPSFGSGP GSQNYATILL PVYAQAANLH LLLLKDADIY GARWGLNQTQ IDQFHSRQQS
     LTQTYTNHCV TAYNDGLAEL RGTTAESWFK YNQYRREMTL TAMDLVALFP YYNLRQYPDG
     TNPQLTREVY TDPIAFDPLE QPTTQLCRSW YINPAFRNHL NFSVLENSLI RPPHLFERLS
     NLQILVNYQT NGSAWRGSRV RYHYLHSSII QEKSYGLLSD PVGANINVQN NDIYQIISQV
     SNFASPVGSS YSVWDTNFYL SSGQVSGISG YTQQGIPAVC LQQRNSTDEL PSLNPEGDII
     RNYSHRLSHI TQYRFQATQS GSPSTVSANL PTCVWTHRDV DLDNTITANQ ITQLPLVKAY
     ELSSGATVVK GPGFTGGDVI RRTNTGGFGA IRVSVTGPLT QRYRIRFRYA STIDFDFFVT
     RGGTTINNFR FTRTMNRGQE SRYESYRTVE FTTPFNFTQS QDIIRTSIQG LSGNGEVYLD
     RIEIIPVNPA REAEEDLEAA KKAARQNLFT RTRDGLQVNV TDYQVDQAAN LVSCLSDEQY
     GHDKKMLLEA VRAAKRLSRE RNLLQDPDFN TINSTEENGW KASNGVTISE GGPFFKGRAL
     QLASARENYP TYIYQKVDAS VLKPYTRYRL DGFVKSSQDL EIDLIHYHKV HLVKNVPDNL
     VSDTYSDGSC SGMNRCEEQQ MVNAQLETEH HHPMDCCEAA QTHEFSSYIN TGDLNASVDQ
     GIWVVLKVRT TDGYATLGNL ELVEVGPLSG ESLEREQRDN AKWNAELGRK RAEIDRVYLA
     AKQAINHLFV DYQDQQLNPE IGLAEINEAS NLVESISGVY SDTLLQIPGI NYEIYTELSD
     RLQQASYLYT SRNAVQNGDF NSGLDSWNTT TDASVQQDGN MHFLVLSHWD AQVSQQLRVN
     PNCKYVLRVT ARKVGGGDGY VTIRDGAHHQ ETLTFNACDY DVNGTYVNDN SYITEEVVFY
     PETKHMWVEV SESEGSFYID SIEFIETQE