ID   CRAA_ACIB2              Reviewed;         413 AA.
AC   D0CCT2;
DT   05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT   24-NOV-2009, sequence version 1.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=Chloramphenicol resistance protein CraA {ECO:0000305};
GN   Name=craA {ECO:0000303|PubMed:19581458};
GN   Synonyms=cmr {ECO:0000312|EMBL:EEX02869.1};
GN   ORFNames=HMPREF0010_02562 {ECO:0000312|EMBL:EEX02869.1};
OS   Acinetobacter baumannii (strain ATCC 19606 / DSM 30007 / JCM 6841 / CCUG
OS   19606 / CIP 70.34 / NBRC 109757 / NCIMB 12457 / NCTC 12156 / 81).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Moraxellales; Moraxellaceae;
OC   Acinetobacter; Acinetobacter calcoaceticus/baumannii complex.
OX   NCBI_TaxID=575584;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19606 / DSM 30007 / JCM 6841 / CCUG 19606 / CIP 70.34 / NBRC
RC   109757 / NCIMB 12457 / NCTC 12156 / 81;
RX   PubMed=23144699; DOI=10.1371/journal.pone.0046984;
RA   Peleg A.Y., de Breij A., Adams M.D., Cerqueira G.M., Mocali S.,
RA   Galardini M., Nibbering P.H., Earl A.M., Ward D.V., Paterson D.L.,
RA   Seifert H., Dijkshoorn L.;
RT   "The success of Acinetobacter species; genetic, metabolic and virulence
RT   attributes.";
RL   PLoS ONE 7:E46984-E46984(2012).
RN   [2]
RP   FUNCTION IN CHLORAMPHENICOL RESISTANCE, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 19606 / DSM 30007 / JCM 6841 / CCUG 19606 / CIP 70.34 / NBRC
RC   109757 / NCIMB 12457 / NCTC 12156 / 81;
RX   PubMed=19581458; DOI=10.1128/aac.00584-09;
RA   Roca I., Marti S., Espinal P., Martinez P., Gibert I., Vila J.;
RT   "CraA, a major facilitator superfamily efflux pump associated with
RT   chloramphenicol resistance in Acinetobacter baumannii.";
RL   Antimicrob. Agents Chemother. 53:4013-4014(2009).
CC   -!- FUNCTION: Efflux pump that mediates resistance to chloramphenicol.
CC       {ECO:0000269|PubMed:19581458}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Mutant shows a strong decrease in chloramphenicol
CC       resistance. Mutation does not affect susceptibility to quinolones,
CC       tetracyclines, aminoglycosides and imipenem.
CC       {ECO:0000269|PubMed:19581458}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       {ECO:0000305}.
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DR   EMBL; GG704576; EEX02869.1; -; Genomic_DNA.
DR   AlphaFoldDB; D0CCT2; -.
DR   SMR; D0CCT2; -.
DR   TCDB; 2.A.1.2.51; the major facilitator superfamily (mfs).
DR   EnsemblBacteria; EEX02869; EEX02869; HMPREF0010_02562.
DR   Proteomes; UP000005740; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR005829; Sugar_transporter_CS.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..413
FT                   /note="Chloramphenicol resistance protein CraA"
FT                   /id="PRO_0000445960"
FT   TRANSMEM        18..38
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        55..75
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        84..104
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        110..130
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        147..167
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        170..190
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        228..248
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        260..280
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        289..309
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        312..332
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        349..369
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        373..393
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   413 AA;  45882 MW;  2F7154C960F905EF CRC64;
     MYKLMKNIQT TALNRTTLMF PLALVLFEFA VYIGNDLIQP AMLAITEDFG VSATWAPSSM
     SFYLLGGASV AWLLGPLSDR LGRKKVLLSG VLFFALCCFL ILLTRQIEHF LTLRFLQGIG
     LSVISAVGYA AIQENFAERD AIKVMALMAN ISLLAPLLGP VLGAFLIDYV SWHWGFVAIA
     LLALLSWVGL KKQMPSHKVS VTKQPFSYLF DDFKKVFSNR QFLGLTLALP LVGMPLMLWI
     ALSPIILVDE LKLTSVQYGL AQFPVFLGLI VGNIVLIKII DRLALGKTVL IGLPIMLTGT
     LILILGVVWQ AYLIPCLLIG MTLICFGEGI SFSVLYRFAL MSSEVSKGTV AAAVSMLLMT
     SFFAMIELVR YLYTQFHLWA FVLSAFAFIA LWFTQPRLAL KREMQERVAQ DLH