CRAC1_HUMAN
ID CRAC1_HUMAN Reviewed; 661 AA.
AC Q9NQ79; B1ALN4; Q5T4F8; Q8N4H6; Q8TE52; Q9NQ78; Q9NQ80; Q9NW46;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Cartilage acidic protein 1;
DE AltName: Full=68 kDa chondrocyte-expressed protein;
DE Short=CEP-68;
DE AltName: Full=ASPIC;
DE Flags: Precursor;
GN Name=CRTAC1; Synonyms=ASPIC1, CEP68;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Cartilage;
RA Bolton M.C., Wait R., Saklatvala J.;
RT "Cloning of ASPIC, a novel protein secreted by human normal and
RT osteoarthritic cartilage, identified by 2D electrophoresis and mass
RT spectrometry.";
RL Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 105-661 (ISOFORM 3).
RC TISSUE=Synovium;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164054; DOI=10.1038/nature02462;
RA Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L.,
RA Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K.,
RA Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L.,
RA Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P.,
RA Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N.,
RA Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A.,
RA Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C.,
RA Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D.,
RA Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C.,
RA Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K.,
RA Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A.,
RA Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S.,
RA McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S.,
RA Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V.,
RA Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A.,
RA Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M.,
RA Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A.,
RA Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P.,
RA Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y.,
RA Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D.,
RA Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 10.";
RL Nature 429:375-381(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 9-661 (ISOFORMS 1 AND 2), INDUCTION, AND
RP TISSUE SPECIFICITY.
RC TISSUE=Brain, and Cartilage;
RX PubMed=11139377; DOI=10.1042/0264-6021:3530169;
RA Steck E., Benz K., Lorenz H., Loew M., Gress T., Richter W.;
RT "Chondrocyte expressed protein-68 (CEP-68), a novel human marker gene for
RT cultured chondrocytes.";
RL Biochem. J. 353:169-174(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 129-661 (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 608-661 (ISOFORM 1), PARTIAL NUCLEOTIDE
RP SEQUENCE [MRNA] (ISOFORM 2), EXON SHARING WITH GOLGA7B (ISOFORM 1),
RP GLYCOSYLATION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=17074475; DOI=10.1016/j.matbio.2006.09.006;
RA Steck E., Braeun J., Pelttari K., Kadel S., Kalbacher H., Richter W.;
RT "Chondrocyte secreted CRTAC1: a glycosylated extracellular matrix molecule
RT of human articular cartilage.";
RL Matrix Biol. 26:30-41(2007).
CC -!- INTERACTION:
CC Q9NQ79; P29972: AQP1; NbExp=3; IntAct=EBI-10205543, EBI-745213;
CC Q9NQ79; Q15555: MAPRE2; NbExp=7; IntAct=EBI-10205543, EBI-739717;
CC Q9NQ79; Q9UPY8: MAPRE3; NbExp=3; IntAct=EBI-10205543, EBI-726739;
CC Q9NQ79; Q8NDC4: MORN4; NbExp=3; IntAct=EBI-10205543, EBI-10269566;
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix {ECO:0000269|PubMed:17074475}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=CRTAC1-A;
CC IsoId=Q9NQ79-1; Sequence=Displayed;
CC Name=2; Synonyms=CRTAC1-B;
CC IsoId=Q9NQ79-2; Sequence=VSP_010894, VSP_010895;
CC Name=3;
CC IsoId=Q9NQ79-3; Sequence=VSP_010893;
CC -!- TISSUE SPECIFICITY: Expressed in the interterritorial matrix of
CC articular deep zone cartilage (at protein level). Isoform 1 and isoform
CC 2 are expressed in brain. Isoform 1 is detected in lung and
CC chondrocytes. Detected in cartilage, bone, cultured chondrocytes and
CC lung, and at low levels in heart. Not detected in osteoblasts.
CC {ECO:0000269|PubMed:11139377, ECO:0000269|PubMed:17074475}.
CC -!- INDUCTION: Up-regulated by BMP4 (at protein level). Up-regulated in
CC mesenchymal stem cells undergoing chondrogenic differentiation and by
CC BMP4. {ECO:0000269|PubMed:11139377}.
CC -!- PTM: O-glycosylated. {ECO:0000269|PubMed:17074475}.
CC -!- MISCELLANEOUS: [Isoform 1]: Shares an exon with the neighboring tail-
CC to-tail oriented gene GOLGA7B.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH34245.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAA91540.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB98268.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB98269.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AJ276171; CAB98267.1; -; mRNA.
DR EMBL; AJ276171; CAB98268.1; ALT_INIT; mRNA.
DR EMBL; AJ276171; CAB98269.1; ALT_INIT; mRNA.
DR EMBL; AK001182; BAA91540.1; ALT_INIT; mRNA.
DR EMBL; AK292084; BAF84773.1; -; mRNA.
DR EMBL; AL139239; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL358938; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AJ279016; CAC08451.1; -; mRNA.
DR EMBL; AJ421515; CAD13394.1; -; mRNA.
DR EMBL; BC034245; AAH34245.1; ALT_INIT; mRNA.
DR CCDS; CCDS31266.1; -. [Q9NQ79-1]
DR CCDS; CCDS55723.1; -. [Q9NQ79-2]
DR RefSeq; NP_001193457.1; NM_001206528.2. [Q9NQ79-2]
DR RefSeq; NP_060528.3; NM_018058.6. [Q9NQ79-1]
DR AlphaFoldDB; Q9NQ79; -.
DR BioGRID; 120427; 24.
DR IntAct; Q9NQ79; 20.
DR STRING; 9606.ENSP00000359629; -.
DR GlyGen; Q9NQ79; 6 sites, 2 O-linked glycans (1 site).
DR iPTMnet; Q9NQ79; -.
DR PhosphoSitePlus; Q9NQ79; -.
DR BioMuta; CRTAC1; -.
DR DMDM; 50400672; -.
DR jPOST; Q9NQ79; -.
DR MassIVE; Q9NQ79; -.
DR PaxDb; Q9NQ79; -.
DR PeptideAtlas; Q9NQ79; -.
DR PRIDE; Q9NQ79; -.
DR ProteomicsDB; 82098; -. [Q9NQ79-1]
DR ProteomicsDB; 82099; -. [Q9NQ79-2]
DR ProteomicsDB; 82100; -. [Q9NQ79-3]
DR Antibodypedia; 2172; 164 antibodies from 27 providers.
DR DNASU; 55118; -.
DR Ensembl; ENST00000370591.6; ENSP00000359623.2; ENSG00000095713.14. [Q9NQ79-2]
DR Ensembl; ENST00000370597.8; ENSP00000359629.3; ENSG00000095713.14. [Q9NQ79-1]
DR GeneID; 55118; -.
DR KEGG; hsa:55118; -.
DR MANE-Select; ENST00000370597.8; ENSP00000359629.3; NM_018058.7; NP_060528.3.
DR UCSC; uc001kou.2; human. [Q9NQ79-1]
DR CTD; 55118; -.
DR DisGeNET; 55118; -.
DR GeneCards; CRTAC1; -.
DR HGNC; HGNC:14882; CRTAC1.
DR HPA; ENSG00000095713; Tissue enhanced (brain, fallopian tube, lung, seminal vesicle).
DR MIM; 606276; gene.
DR neXtProt; NX_Q9NQ79; -.
DR OpenTargets; ENSG00000095713; -.
DR PharmGKB; PA26899; -.
DR VEuPathDB; HostDB:ENSG00000095713; -.
DR eggNOG; ENOG502QQ5V; Eukaryota.
DR GeneTree; ENSGT00390000013726; -.
DR HOGENOM; CLU_027473_0_0_1; -.
DR InParanoid; Q9NQ79; -.
DR OMA; FFVANYG; -.
DR OrthoDB; 986490at2759; -.
DR PhylomeDB; Q9NQ79; -.
DR TreeFam; TF333171; -.
DR PathwayCommons; Q9NQ79; -.
DR SignaLink; Q9NQ79; -.
DR BioGRID-ORCS; 55118; 13 hits in 1064 CRISPR screens.
DR ChiTaRS; CRTAC1; human.
DR GenomeRNAi; 55118; -.
DR Pharos; Q9NQ79; Tbio.
DR PRO; PR:Q9NQ79; -.
DR Proteomes; UP000005640; Chromosome 10.
DR RNAct; Q9NQ79; protein.
DR Bgee; ENSG00000095713; Expressed in tendon of biceps brachii and 159 other tissues.
DR ExpressionAtlas; Q9NQ79; baseline and differential.
DR Genevisible; Q9NQ79; HS.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0007413; P:axonal fasciculation; IBA:GO_Central.
DR Gene3D; 2.130.10.130; -; 1.
DR InterPro; IPR027039; Crtac1.
DR InterPro; IPR001881; EGF-like_Ca-bd_dom.
DR InterPro; IPR018097; EGF_Ca-bd_CS.
DR InterPro; IPR013517; FG-GAP.
DR InterPro; IPR028994; Integrin_alpha_N.
DR InterPro; IPR011519; UnbV_ASPIC.
DR PANTHER; PTHR16026:SF4; PTHR16026:SF4; 1.
DR Pfam; PF07645; EGF_CA; 1.
DR Pfam; PF13517; FG-GAP_3; 2.
DR Pfam; PF07593; UnbV_ASPIC; 1.
DR SMART; SM00179; EGF_CA; 1.
DR SUPFAM; SSF69318; SSF69318; 1.
DR PROSITE; PS01187; EGF_CA; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Disulfide bond; EGF-like domain;
KW Extracellular matrix; Glycoprotein; Reference proteome; Repeat; Secreted;
KW Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..661
FT /note="Cartilage acidic protein 1"
FT /id="PRO_0000007497"
FT REPEAT 46..88
FT /note="FG-GAP 1; atypical"
FT REPEAT 105..147
FT /note="FG-GAP 2; atypical"
FT REPEAT 283..333
FT /note="FG-GAP 3; atypical"
FT REPEAT 395..437
FT /note="FG-GAP 4; atypical"
FT DOMAIN 559..605
FT /note="EGF-like"
FT CARBOHYD 608
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000255"
FT CARBOHYD 618
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000255"
FT CARBOHYD 619
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000255"
FT CARBOHYD 621
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000255"
FT CARBOHYD 626
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000255"
FT DISULFID 563..577
FT /evidence="ECO:0000255"
FT DISULFID 570..586
FT /evidence="ECO:0000255"
FT DISULFID 592..605
FT /evidence="ECO:0000255"
FT VAR_SEQ 545..661
FT /note="CGQGFSQQENGHCMDTNECIQFPFVCPRDKPVCVNTYGSYRCRTNKKCSRGY
FT EPNEDGTACVGTLGQSPGPRPTTPTAAAATAAAAAAAGAATAAPVLVDGDLNLGSVVKE
FT SCEPSC -> TPMNASSSHSCALETSPYVSTPMEATGAGPTRSAVGATSPTRMAQPAWG
FT LSASHRAPAPPPPPLLLPLPLLLPLLELPLLHRSS (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_010893"
FT VAR_SEQ 607..645
FT /note="GTLGQSPGPRPTTPTAAAATAAAAAAAGAATAAPVLVDG -> AQVAFLGGY
FT SSAASRISEPLSRASYLSLGLGLCLQLYAL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11139377"
FT /id="VSP_010894"
FT VAR_SEQ 646..661
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11139377"
FT /id="VSP_010895"
FT VARIANT 253
FT /note="A -> T (in dbSNP:rs35853031)"
FT /id="VAR_048972"
FT VARIANT 569
FT /note="V -> M (in dbSNP:rs2297935)"
FT /id="VAR_048973"
FT VARIANT 658
FT /note="E -> K (in dbSNP:rs56007204)"
FT /id="VAR_061152"
FT CONFLICT 454
FT /note="F -> V (in Ref. 2; BAA91540)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 661 AA; 71421 MW; 2EF75321817F0D01 CRC64;
MAPSADPGMS RMLPFLLLLW FLPITEGSQR AEPMFTAVTN SVLPPDYDSN PTQLNYGVAV
TDVDHDGDFE IVVAGYNGPN LVLKYDRAQK RLVNIAVDER SSPYYALRDR QGNAIGVTAC
DIDGDGREEI YFLNTNNAFS GVATYTDKLF KFRNNRWEDI LSDEVNVARG VASLFAGRSV
ACVDRKGSGR YSIYIANYAY GNVGPDALIE MDPEASDLSR GILALRDVAA EAGVSKYTGG
RGVSVGPILS SSASDIFCDN ENGPNFLFHN RGDGTFVDAA ASAGVDDPHQ HGRGVALADF
NRDGKVDIVY GNWNGPHRLY LQMSTHGKVR FRDIASPKFS MPSPVRTVIT ADFDNDQELE
IFFNNIAYRS SSANRLFRVI RREHGDPLIE ELNPGDALEP EGRGTGGVVT DFDGDGMLDL
ILSHGESMAQ PLSVFRGNQG FNNNWLRVVP RTRFGAFARG AKVVLYTKKS GAHLRIIDGG
SGYLCEMEPV AHFGLGKDEA SSVEVTWPDG KMVSRNVASG EMNSVLEILY PRDEDTLQDP
APLECGQGFS QQENGHCMDT NECIQFPFVC PRDKPVCVNT YGSYRCRTNK KCSRGYEPNE
DGTACVGTLG QSPGPRPTTP TAAAATAAAA AAAGAATAAP VLVDGDLNLG SVVKESCEPS
C
