CRAM_TRYBB
ID CRAM_TRYBB Reviewed; 945 AA.
AC Q03650;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 71.
DE RecName: Full=Cysteine-rich, acidic integral membrane protein;
DE Flags: Precursor;
GN Name=CRAM;
OS Trypanosoma brucei brucei.
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX NCBI_TaxID=5702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Treu667But55;
RX PubMed=1697030; DOI=10.1128/mcb.10.9.4506-4517.1990;
RA Lee G.S.M., Bihain B.E., Russell D.G., Deckelbaum R.J., Ploeg L.H.;
RT "Characterization of a cDNA encoding a cysteine-rich cell surface protein
RT located in the flagellar pocket of the protozoan Trypanosoma brucei.";
RL Mol. Cell. Biol. 10:4506-4517(1990).
CC -!- FUNCTION: Supposed to function as cell surface receptor. Possibly
CC involved in receptor-mediated endocytosis.
CC -!- SUBCELLULAR LOCATION: Flagellar pocket. Cell membrane {ECO:0000305};
CC Single-pass membrane protein {ECO:0000305}. Note=Concentrated in the
CC flagellar pocket.
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DR EMBL; M55686; AAA30169.1; -; mRNA.
DR EMBL; M61131; AAA30170.1; ALT_SEQ; mRNA.
DR AlphaFoldDB; Q03650; -.
DR TCDB; 9.B.87.1.23; the selenoprotein p receptor (selp-receptor) family.
DR GO; GO:0020016; C:ciliary pocket; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
DR InterPro; IPR009745; CRAM_rpt.
DR Pfam; PF07016; CRAM_rpt; 65.
PE 2: Evidence at transcript level;
KW Cell membrane; Endocytosis; Glycoprotein; Membrane; Repeat; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..945
FT /note="Cysteine-rich, acidic integral membrane protein"
FT /evidence="ECO:0000255"
FT /id="PRO_0000020993"
FT TOPO_DOM 24..882
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 883..903
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 904..945
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 40..51
FT /note="1"
FT REPEAT 52..63
FT /note="2"
FT REPEAT 64..75
FT /note="3"
FT REPEAT 76..87
FT /note="4"
FT REPEAT 88..99
FT /note="5"
FT REPEAT 100..111
FT /note="6"
FT REPEAT 112..123
FT /note="7"
FT REPEAT 124..135
FT /note="8"
FT REPEAT 136..147
FT /note="9"
FT REPEAT 148..159
FT /note="10"
FT REPEAT 160..171
FT /note="11"
FT REPEAT 172..183
FT /note="12"
FT REPEAT 184..195
FT /note="13"
FT REPEAT 196..207
FT /note="14"
FT REPEAT 208..219
FT /note="15"
FT REPEAT 220..231
FT /note="16"
FT REPEAT 232..243
FT /note="17"
FT REPEAT 244..255
FT /note="18"
FT REPEAT 256..267
FT /note="19"
FT REPEAT 268..279
FT /note="20"
FT REPEAT 280..291
FT /note="21"
FT REPEAT 292..303
FT /note="22"
FT REPEAT 304..315
FT /note="23"
FT REPEAT 316..327
FT /note="24"
FT REPEAT 328..339
FT /note="25"
FT REPEAT 340..351
FT /note="26"
FT REPEAT 352..363
FT /note="27"
FT REPEAT 364..375
FT /note="28"
FT REPEAT 376..387
FT /note="29"
FT REPEAT 388..399
FT /note="30"
FT REPEAT 400..411
FT /note="31"
FT REPEAT 412..423
FT /note="32"
FT REPEAT 424..435
FT /note="33"
FT REPEAT 436..447
FT /note="34"
FT REPEAT 448..459
FT /note="35"
FT REPEAT 460..471
FT /note="36"
FT REPEAT 472..483
FT /note="37"
FT REPEAT 484..495
FT /note="38"
FT REPEAT 496..507
FT /note="39"
FT REPEAT 508..519
FT /note="40"
FT REPEAT 520..531
FT /note="41"
FT REPEAT 532..543
FT /note="42"
FT REPEAT 544..555
FT /note="43"
FT REPEAT 556..567
FT /note="44"
FT REPEAT 568..579
FT /note="45"
FT REPEAT 580..591
FT /note="46"
FT REPEAT 592..603
FT /note="47"
FT REPEAT 604..615
FT /note="48"
FT REPEAT 616..627
FT /note="49"
FT REPEAT 628..639
FT /note="50"
FT REPEAT 640..651
FT /note="51"
FT REPEAT 652..663
FT /note="52"
FT REPEAT 664..675
FT /note="53"
FT REPEAT 676..687
FT /note="54"
FT REPEAT 688..699
FT /note="55"
FT REPEAT 700..711
FT /note="56"
FT REPEAT 712..723
FT /note="57"
FT REPEAT 724..735
FT /note="58"
FT REPEAT 736..747
FT /note="59"
FT REPEAT 748..759
FT /note="60"
FT REPEAT 760..771
FT /note="61"
FT REPEAT 772..783
FT /note="62"
FT REPEAT 784..795
FT /note="63"
FT REPEAT 796..807
FT /note="64"
FT REPEAT 808..819
FT /note="65"
FT REPEAT 820..831
FT /note="66"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 40..831
FT /note="66 X 12 AA tandem repeats of D-D-C-[ND]-I-T-G-D-G-N-
FT E-T"
FT CARBOHYD 34
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 43
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 67
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 79
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 91
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 103
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 127
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 139
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 151
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 175
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 199
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 211
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 223
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 235
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 247
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 259
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 283
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 295
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 307
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 319
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 331
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 343
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 367
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 379
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 391
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 403
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 415
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 439
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 463
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 475
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 487
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 499
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 511
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 523
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 547
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 559
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 571
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 583
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 595
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 619
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 643
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 655
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 667
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 679
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 691
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 703
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 727
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 739
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 751
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 763
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 775
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 799
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 823
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 945 AA; 100382 MW; 2F57C893ACA4129E CRC64;
MGNEAGPIFE ESNAEVGTPP ADAVHDDFFF DYKNATGYAD DCNITGDCNE TDDCDITGDC
NETDDCNITG DCNETDDCNI TGDCNETDDC NITGDCNETD DCNITGDCNE TDDCDITGDC
NETDDCNITG DCNETDDCNI TGDCNETDDC NITGDCNETD DCDITGDCNE TDDCNITGDC
NETDDCDITG DCNETDDCNI TGDCNETDDC NITGDCNETD DCNITGDCNE TDDCNITGDC
NETDDCNITG DCNETDDCNI TGDCNETDDC DITGDCNETD DCNITGDCNE TDDCNITGDC
NETDDCNITG DCNETDDCNI TGDCNETDDC NITGDCNETD DCNITGDCNE TDDCDITGDC
NETDDCNITG DCNETDDCNI TGDCNETDDC NITGDCNETD DCNITGDCNE TDDCNITGDC
NETDDCDITG DCNETDDCNI TGDCNETDDC DITGDCNETD DCNITGDCNE TDDCNITGDC
NETDDCNITG DCNETDDCNI TGDCNETDDC NITGDCNETD DCNITGDCNE TDDCDITGDC
NETDDCNITG DCNETDDCNI TGDCNETDDC NITGDCNETD DCNITGDCNE TDDCNITGDC
NETDDCDITG DCNETDDCNI TGDCNETDDC DITGDCNETD DCNITGDCNE TDDCNITGDC
NETDDCNITG DCNETDDCNI TGDCNETDDC NITGDCNETD DCNITGDCNE TDDCDITGDC
NETDDCNITG DCNETDDCNI TGDCNETDDC NITGDCNETD DCNITGDCNE TDDCNITGDC
NETDDCDITG DCNETDDCNI TGDCNETDDC DITGDCNETD DCNITGDCNE TEVSDAADGT
DGMFLKSSSS LKLVALCDGC PTEDSPKSSN AKGKGSSVSA GLLLLAGSTF LVLAVGLSAV
LFLGRERQNA VVICDNEVMM EEVPGCLSDA SFAVPVTQSS DEARP
