ID   CRAS1_URTCR             Reviewed;          79 AA.
AC   A0A1X9QHL1;
DT   07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT   30-AUG-2017, sequence version 1.
DT   25-MAY-2022, entry version 17.
DE   RecName: Full=Crassicorin-I {ECO:0000303|PubMed:28796463};
DE   AltName: Full=BDS-like antimicrobial peptide {ECO:0000303|PubMed:28796463};
DE            Short=BDS-like AMP {ECO:0000303|PubMed:28796463};
DE   Flags: Precursor;
OS   Urticina crassicornis (Mottled anemone) (Tealia crassicornis).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Actiniidae; Urticina.
OX   NCBI_TaxID=45621;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 40-75, FUNCTION,
RP   SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY, TOXIC DOSE,
RP   RECOMBINANT EXPRESSION, AND 3D-STRUCTURE MODELING.
RX   PubMed=28796463; DOI=10.1111/febs.14194;
RA   Kim C.H., Lee Y.J., Go H.J., Oh H.Y., Lee T.K., Park J.B., Park N.G.;
RT   "Defensin-neurotoxin dyad in a basally branching metazoan sea anemone.";
RL   FEBS J. 284:3320-3338(2017).
CC   -!- FUNCTION: Peptide with both antimicrobial and neurotoxin activities.
CC       Cationic AMP with antibacterial activity against both Gram-positive
CC       bacteria (B.subtilis, MIC=11.49 ug/mL) and Gram-negative bacteria
CC       (E.coli (MIC=12.21 ug/mL) and S.enterica (MIC=11.95 ug/mL))
CC       (PubMed:28796463). Shows no significant antimicrobial activity against
CC       bacteria S.aureus and P.aeruginosa, as well as the fungus C.albicans
CC       (PubMed:28796463). In vivo, induces reversible paralytic activity
CC       towards the shrimp P.paucidens (PubMed:28796463). May act by impairing
CC       sodium or potassium channels in the prey (Probable).
CC       {ECO:0000269|PubMed:28796463, ECO:0000305|PubMed:28796463}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:28796463}.
CC       Nematocyst {ECO:0000305|PubMed:28796463}.
CC   -!- TISSUE SPECIFICITY: Highly expressed by the mesenteries. Moderately
CC       expressed by the pharynx. Weakly expressed by the gonad and pedal disk.
CC       No expression in tentacle. {ECO:0000269|PubMed:28796463}.
CC   -!- INDUCTION: Transcriptionally up-regulated by immune challenge.
CC       {ECO:0000269|PubMed:28796463}.
CC   -!- MASS SPECTROMETRY: Mass=4313.8; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:28796463};
CC   -!- TOXIC DOSE: PD(50) is 0.84 +- 0.16 ug/g body weight when injected into
CC       the shrimp P.paucidens. The minimum paralytic dose is 2 ug/g body
CC       weight shrimp body weight. {ECO:0000269|PubMed:28796463}.
CC   -!- SIMILARITY: Belongs to the sea anemone type 3 (BDS) potassium channel
CC       toxin family. {ECO:0000305}.
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DR   EMBL; KY229683; ARQ30160.1; -; mRNA.
DR   AlphaFoldDB; A0A1X9QHL1; -.
DR   SMR; A0A1X9QHL1; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   Gene3D; 2.20.20.10; -; 1.
DR   InterPro; IPR012414; BDS_K_chnl_tox.
DR   InterPro; IPR023355; Myo_ane_neurotoxin_sf.
DR   Pfam; PF07936; Defensin_4; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Cleavage on pair of basic residues;
KW   Direct protein sequencing; Disulfide bond; Immunity; Innate immunity;
KW   Ion channel impairing toxin; Nematocyst; Neurotoxin; Secreted; Signal;
KW   Toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..39
FT                   /evidence="ECO:0000250|UniProtKB:G0W2H8"
FT                   /id="PRO_0000452124"
FT   CHAIN           40..79
FT                   /note="Crassicorin-I"
FT                   /evidence="ECO:0000269|PubMed:28796463"
FT                   /id="PRO_5011001360"
FT   DISULFID        43..76
FT                   /evidence="ECO:0000250|UniProtKB:P61541"
FT   DISULFID        45..69
FT                   /evidence="ECO:0000250|UniProtKB:P61541"
FT   DISULFID        59..77
FT                   /evidence="ECO:0000250|UniProtKB:P61541"
SQ   SEQUENCE   79 AA;  8754 MW;  EE215C35A639C8FF CRC64;
     MKLFLVSIVL VGMLVLAAAR PERDIDSFDE QEEKGFVKRG ASCDCHPFVG TYWFGISNCP
     SGHGYRKKCA SFFGVCCVK